SAP_MYCTU
ID SAP_MYCTU Reviewed; 237 AA.
AC O07802; F2GDI0; I6YD44; Q7D4T3;
DT 29-APR-2015, integrated into UniProtKB/Swiss-Prot.
DT 01-AUG-1998, sequence version 2.
DT 03-AUG-2022, entry version 116.
DE RecName: Full=Sulfolipid-1 exporter Sap {ECO:0000305};
DE AltName: Full=Sulfolipid-1-addressing protein {ECO:0000303|PubMed:22194604};
GN Name=sap {ECO:0000303|PubMed:22194604};
GN OrderedLocusNames=Rv3821 {ECO:0000312|EMBL:CCP46650.1},
GN RVBD_3821 {ECO:0000312|EMBL:AFN51847.1};
GN ORFNames=LH57_20825 {ECO:0000312|EMBL:AIR16615.1},
GN P425_03979 {ECO:0000312|EMBL:KBJ24898.1};
OS Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv).
OC Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC Mycobacterium; Mycobacterium tuberculosis complex.
OX NCBI_TaxID=83332;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 25618 / H37Rv;
RX PubMed=9634230; DOI=10.1038/31159;
RA Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E.,
RA Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K.,
RA Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K.,
RA Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K.,
RA Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J.,
RA Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S.,
RA Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S.,
RA Barrell B.G.;
RT "Deciphering the biology of Mycobacterium tuberculosis from the complete
RT genome sequence.";
RL Nature 393:537-544(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 25618 / H37Rv;
RG The Broad Institute Genome Sequencing Platform;
RA Galagan J., Kreiswirth B., Dobos K., Fortune S., Fitzgerald M., Young S.K.,
RA Zeng Q., Gargeya S., Abouelleil A., Alvarado L., Berlin A.M., Chapman S.B.,
RA Gainer-Dewar J., Goldberg J., Gnerre S., Griggs A., Gujja S., Hansen M.,
RA Howarth C., Imamovic A., Larimer J., McCowan C., Murphy C., Pearson M.,
RA Poon T., Priest M., Roberts A., Saif S., Shea T., Sykes S., Wortman J.,
RA Nusbaum C., Birren B.;
RT "The genome sequence of Mycobacterium tuberculosis H37Rv.";
RL Submitted (NOV-2013) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 25618 / H37Rv;
RG The Broad Institute Genomics Platform;
RG The Broad Institute Genome Sequencing Center for Infectious Disease;
RA Earl A.M., Kreiswirth B., Gomez J., Victor T., Desjardins C., Abeel T.,
RA Young S., Zeng Q., Gargeya S., Abouelleil A., Alvarado L., Chapman S.B.,
RA Gainer-Dewar J., Goldberg J., Griggs A., Gujja S., Hansen M., Howarth C.,
RA Imamovic A., Larimer J., Murphy C., Naylor J., Pearson M., Poon T.W.,
RA Priest M., Roberts A., Saif S., Shea T., Sykes S., Wortman J., Nusbaum C.,
RA Birren B.;
RT "The genome sequence of Mycobacterium tuberculosis H37Rv.";
RL Submitted (APR-2014) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 27294 / TMC 102 / H37Rv;
RA Hazbon M.H., Riojas M.A., Damon A.M., Alalade R.O., Cantwell B.J.,
RA Monaco A., King S., Sohrabi A.;
RT "Phylogenetic analysis of Mycobacterial species using whole genome
RT sequences.";
RL Submitted (SEP-2014) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP FUNCTION IN TRANSPORT, SUBCELLULAR LOCATION, AND DISRUPTION PHENOTYPE.
RC STRAIN=ATCC 35801 / TMC 107 / Erdman;
RX PubMed=22194604; DOI=10.1074/jbc.m111.315473;
RA Seeliger J.C., Holsclaw C.M., Schelle M.W., Botyanszki Z., Gilmore S.A.,
RA Tully S.E., Niederweis M., Cravatt B.F., Leary J.A., Bertozzi C.R.;
RT "Elucidation and chemical modulation of sulfolipid-1 biosynthesis in
RT Mycobacterium tuberculosis.";
RL J. Biol. Chem. 287:7990-8000(2012).
CC -!- FUNCTION: Required for the transport across the inner membrane of
CC sulfolipid-1 (SL-1), which is a major cell wall lipid of pathogenic
CC mycobacteria. Could also transport SL1278 (2-palmitoyl-3-(C43)-
CC phthioceranyl-alpha, alpha'-D-trehalose-2'-sulfate), which is the
CC precursor of SL-1. May potentiate SL-1 levels and confer specificity
CC for sulfolipids over structurally similar glycolipids.
CC {ECO:0000269|PubMed:22194604}.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane
CC {ECO:0000305|PubMed:22194604}; Multi-pass membrane protein
CC {ECO:0000255}.
CC -!- DISRUPTION PHENOTYPE: Accumulates the precursor SL1278. Can produce SL-
CC 1, albeit at reduced levels. {ECO:0000269|PubMed:22194604}.
CC -!- SIMILARITY: Belongs to the peptidoglycolipid addressing protein (GAP)
CC (TC 2.A.116) family. {ECO:0000305}.
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DR EMBL; CP003248; AFN51847.1; -; Genomic_DNA.
DR EMBL; CP009480; AIR16615.1; -; Genomic_DNA.
DR EMBL; AL123456; CCP46650.1; -; Genomic_DNA.
DR EMBL; JLDD01000048; KBJ24898.1; -; Genomic_DNA.
DR RefSeq; NP_218338.1; NC_000962.3.
DR RefSeq; WP_003420848.1; NZ_NVQJ01000022.1.
DR AlphaFoldDB; O07802; -.
DR STRING; 83332.Rv3821; -.
DR TCDB; 2.A.116.1.2; the peptidoglycolipid addressing protein (gap) family.
DR PaxDb; O07802; -.
DR PRIDE; O07802; -.
DR DNASU; 886141; -.
DR GeneID; 886141; -.
DR KEGG; mtu:Rv3821; -.
DR KEGG; mtv:RVBD_3821; -.
DR PATRIC; fig|83332.111.peg.4247; -.
DR TubercuList; Rv3821; -.
DR eggNOG; ENOG5033YWV; Bacteria.
DR HOGENOM; CLU_068662_0_0_11; -.
DR OMA; VSFEPFR; -.
DR PhylomeDB; O07802; -.
DR Proteomes; UP000001584; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0071555; P:cell wall organization; IEA:UniProtKB-KW.
DR GO; GO:0006869; P:lipid transport; IEA:UniProtKB-KW.
DR InterPro; IPR021315; Gap/Sap.
DR Pfam; PF11139; SfLAP; 1.
PE 1: Evidence at protein level;
KW Cell inner membrane; Cell membrane; Cell wall biogenesis/degradation;
KW Lipid transport; Membrane; Reference proteome; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..237
FT /note="Sulfolipid-1 exporter Sap"
FT /id="PRO_0000432808"
FT TRANSMEM 5..25
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 38..58
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 66..86
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 141..161
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 171..191
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 217..237
FT /note="Helical"
FT /evidence="ECO:0000255"
SQ SEQUENCE 237 AA; 24627 MW; DD2F9721F816A562 CRC64;
MWSTVLVLAL SVICEPVRIG LVVLMLNRRR PLLHLLTFLC GGYTMAGGVA MVTLVVLGAT
PLAGHFSVAE VQIGTGLIAL LIAFALTTNV IGKHVRRATH ARVGDDGGRV LRESVPPSGA
HKLAVRARCF LQGDSLYVAG VSGLGAALPS ANYMGAMAAI LASGATPATQ ALAVVTFNVV
AFTVAEVPLV SYLAAPRKTR AFMAALQSWL RSRSRRDAAL LVAAGGCLML TLGLSNL
