ID   SARAF_BOVIN             Reviewed;         335 AA.
AC   Q08E24;
DT   08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT   31-OCT-2006, sequence version 1.
DT   03-AUG-2022, entry version 74.
DE   RecName: Full=Store-operated calcium entry-associated regulatory factor;
DE            Short=SARAF;
DE            Short=SOCE-associated regulatory factor;
DE   AltName: Full=Transmembrane protein 66;
DE   Flags: Precursor;
GN   Name=SARAF; Synonyms=TMEM66;
OS   Bos taurus (Bovine).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC   Bovinae; Bos.
OX   NCBI_TaxID=9913;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=Hereford; TISSUE=Hippocampus;
RG   NIH - Mammalian Gene Collection (MGC) project;
RL   Submitted (SEP-2006) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Negative regulator of store-operated Ca(2+) entry (SOCE)
CC       involved in protecting cells from Ca(2+) overfilling. In response to
CC       cytosolic Ca(2+) elevation after endoplasmic reticulum Ca(2+)
CC       refilling, promotes a slow inactivation of STIM (STIM1 or STIM2)-
CC       dependent SOCE activity: possibly act by facilitating the
CC       deoligomerization of STIM to efficiently turn off ORAI when the
CC       endoplasmic reticulum lumen is filled with the appropriate Ca(2+)
CC       levels, and thus preventing the overload of the cell with excessive
CC       Ca(2+) ions (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Interacts with STIM1; the interaction is inhibit by th
CC       interaction of STIM1 with EFHB. {ECO:0000250|UniProtKB:Q96BY9}.
CC   -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane {ECO:0000250};
CC       Single-pass type I membrane protein {ECO:0000250}. Note=Translocates to
CC       the endoplasmic reticulum-plasma membrane (ER-PM) region in a STIM1-
CC       dependent manner following cytosolic Ca(2+) elevation. {ECO:0000250}.
CC   -!- DOMAIN: The cytoplasmic C-terminal region mediates interaction with
CC       STIM1, while the N-terminal lumenal region mediates regulation of SOCE
CC       activity. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the SARAF family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; BC123456; AAI23457.1; -; mRNA.
DR   RefSeq; NP_001070324.1; NM_001076856.1.
DR   AlphaFoldDB; Q08E24; -.
DR   SMR; Q08E24; -.
DR   STRING; 9913.ENSBTAP00000018052; -.
DR   PaxDb; Q08E24; -.
DR   PRIDE; Q08E24; -.
DR   Ensembl; ENSBTAT00000018052; ENSBTAP00000018052; ENSBTAG00000013579.
DR   GeneID; 515461; -.
DR   KEGG; bta:515461; -.
DR   CTD; 51669; -.
DR   VEuPathDB; HostDB:ENSBTAG00000013579; -.
DR   VGNC; VGNC:34288; SARAF.
DR   eggNOG; ENOG502QT6Y; Eukaryota.
DR   GeneTree; ENSGT00390000013419; -.
DR   HOGENOM; CLU_046802_0_1_1; -.
DR   InParanoid; Q08E24; -.
DR   OMA; DKWVLKG; -.
DR   OrthoDB; 1402326at2759; -.
DR   TreeFam; TF314811; -.
DR   Proteomes; UP000009136; Chromosome 27.
DR   Bgee; ENSBTAG00000013579; Expressed in prostate gland and 105 other tissues.
DR   ExpressionAtlas; Q08E24; baseline.
DR   GO; GO:0140268; C:endoplasmic reticulum-plasma membrane contact site; ISS:UniProtKB.
DR   GO; GO:0030176; C:integral component of endoplasmic reticulum membrane; ISS:UniProtKB.
DR   GO; GO:0006816; P:calcium ion transport; IEA:UniProtKB-KW.
DR   GO; GO:2001256; P:regulation of store-operated calcium entry; ISS:UniProtKB.
DR   InterPro; IPR009567; SARAF.
DR   PANTHER; PTHR15929; PTHR15929; 1.
DR   Pfam; PF06682; SARAF; 1.
PE   2: Evidence at transcript level;
KW   Calcium; Calcium transport; Endoplasmic reticulum; Ion transport; Membrane;
KW   Reference proteome; Signal; Transmembrane; Transmembrane helix; Transport.
FT   SIGNAL          1..30
FT                   /evidence="ECO:0000255"
FT   CHAIN           31..335
FT                   /note="Store-operated calcium entry-associated regulatory
FT                   factor"
FT                   /id="PRO_0000328114"
FT   TOPO_DOM        31..172
FT                   /note="Lumenal"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        173..193
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        194..335
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   REGION          203..259
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          300..335
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        300..326
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   335 AA;  36314 MW;  D6BBD81212862A0C CRC64;
     MAAAGGPAAA GRCALSSLLL LLLVVGTAQC WDERDRILLR DIKALTLYYD RYTTSRRLEP
     IPQLKCVGGT AGCDSYTPKV IQCQNRGWDG YDVQWECKTD LDVAYKFGKT VVSCEGYESS
     EDQYVLRGSC GLEYQLDYTE LGLKKLRESG KEHGFNSFSN YYSKLYSPES SSLGGVVTIV
     VLLAIAFGVY KFFLSDGHES PPPYSEDPPY SHRYQRFSSS AGPPPAGFKS EFTGPHGATA
     GFGSAFTGQQ GHEHSGPGFW TGLGTGGILG YLFGSNRAAT PFSDSWYPSY PPSYTSTWNS
     RAYSPLRGGP GSSSACSGSE PRTRTASGYG GTRRR