SARAF_DANRE
ID SARAF_DANRE Reviewed; 345 AA.
AC F1QPC3; E9QGQ1; Q08CB8;
DT 16-MAY-2012, integrated into UniProtKB/Swiss-Prot.
DT 03-MAY-2011, sequence version 1.
DT 03-AUG-2022, entry version 58.
DE RecName: Full=Store-operated calcium entry-associated regulatory factor;
DE Short=SARAF;
DE Short=SOCE-associated regulatory factor;
DE AltName: Full=Transmembrane protein 66;
DE Flags: Precursor;
GN Name=saraf; Synonyms=tmem66; ORFNames=zgc:153286;
OS Danio rerio (Zebrafish) (Brachydanio rerio).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC Danionidae; Danioninae; Danio.
OX NCBI_TaxID=7955;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Tuebingen;
RX PubMed=23594743; DOI=10.1038/nature12111;
RA Howe K., Clark M.D., Torroja C.F., Torrance J., Berthelot C., Muffato M.,
RA Collins J.E., Humphray S., McLaren K., Matthews L., McLaren S., Sealy I.,
RA Caccamo M., Churcher C., Scott C., Barrett J.C., Koch R., Rauch G.J.,
RA White S., Chow W., Kilian B., Quintais L.T., Guerra-Assuncao J.A., Zhou Y.,
RA Gu Y., Yen J., Vogel J.H., Eyre T., Redmond S., Banerjee R., Chi J., Fu B.,
RA Langley E., Maguire S.F., Laird G.K., Lloyd D., Kenyon E., Donaldson S.,
RA Sehra H., Almeida-King J., Loveland J., Trevanion S., Jones M., Quail M.,
RA Willey D., Hunt A., Burton J., Sims S., McLay K., Plumb B., Davis J.,
RA Clee C., Oliver K., Clark R., Riddle C., Elliot D., Threadgold G.,
RA Harden G., Ware D., Begum S., Mortimore B., Kerry G., Heath P.,
RA Phillimore B., Tracey A., Corby N., Dunn M., Johnson C., Wood J., Clark S.,
RA Pelan S., Griffiths G., Smith M., Glithero R., Howden P., Barker N.,
RA Lloyd C., Stevens C., Harley J., Holt K., Panagiotidis G., Lovell J.,
RA Beasley H., Henderson C., Gordon D., Auger K., Wright D., Collins J.,
RA Raisen C., Dyer L., Leung K., Robertson L., Ambridge K., Leongamornlert D.,
RA McGuire S., Gilderthorp R., Griffiths C., Manthravadi D., Nichol S.,
RA Barker G., Whitehead S., Kay M., Brown J., Murnane C., Gray E.,
RA Humphries M., Sycamore N., Barker D., Saunders D., Wallis J., Babbage A.,
RA Hammond S., Mashreghi-Mohammadi M., Barr L., Martin S., Wray P.,
RA Ellington A., Matthews N., Ellwood M., Woodmansey R., Clark G., Cooper J.,
RA Tromans A., Grafham D., Skuce C., Pandian R., Andrews R., Harrison E.,
RA Kimberley A., Garnett J., Fosker N., Hall R., Garner P., Kelly D., Bird C.,
RA Palmer S., Gehring I., Berger A., Dooley C.M., Ersan-Urun Z., Eser C.,
RA Geiger H., Geisler M., Karotki L., Kirn A., Konantz J., Konantz M.,
RA Oberlander M., Rudolph-Geiger S., Teucke M., Lanz C., Raddatz G.,
RA Osoegawa K., Zhu B., Rapp A., Widaa S., Langford C., Yang F.,
RA Schuster S.C., Carter N.P., Harrow J., Ning Z., Herrero J., Searle S.M.,
RA Enright A., Geisler R., Plasterk R.H., Lee C., Westerfield M.,
RA de Jong P.J., Zon L.I., Postlethwait J.H., Nusslein-Volhard C.,
RA Hubbard T.J., Roest Crollius H., Rogers J., Stemple D.L.;
RT "The zebrafish reference genome sequence and its relationship to the human
RT genome.";
RL Nature 496:498-503(2013).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Skin;
RG NIH - Zebrafish Gene Collection (ZGC) project;
RL Submitted (SEP-2006) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Negative regulator of store-operated Ca(2+) entry (SOCE)
CC involved in protecting cells from Ca(2+) overfilling. In response to
CC cytosolic Ca(2+) elevation after endoplasmic reticulum Ca(2+)
CC refilling, promotes a slow inactivation of STIM (stim1 or stim2)-
CC dependent SOCE activity (By similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane {ECO:0000250};
CC Single-pass type I membrane protein {ECO:0000250}. Note=Translocates to
CC the endoplasmic reticulum-plasma membrane (ER-PM) region in a STIM1-
CC dependent manner following cytosolic Ca(2+) elevation. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the SARAF family. {ECO:0000305}.
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DR EMBL; BX908747; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC124302; AAI24303.1; -; mRNA.
DR RefSeq; NP_001070057.1; NM_001076589.1.
DR AlphaFoldDB; F1QPC3; -.
DR SMR; F1QPC3; -.
DR STRING; 7955.ENSDARP00000115385; -.
DR PaxDb; F1QPC3; -.
DR Ensembl; ENSDART00000103403; ENSDARP00000094179; ENSDARG00000070452.
DR GeneID; 767649; -.
DR KEGG; dre:767649; -.
DR CTD; 51669; -.
DR ZFIN; ZDB-GENE-060929-208; saraf.
DR eggNOG; ENOG502QT6Y; Eukaryota.
DR GeneTree; ENSGT00390000013419; -.
DR HOGENOM; CLU_046802_0_1_1; -.
DR InParanoid; F1QPC3; -.
DR OMA; DKWVLKG; -.
DR TreeFam; TF314811; -.
DR PRO; PR:F1QPC3; -.
DR Proteomes; UP000000437; Genome assembly.
DR Proteomes; UP000814640; Chromosome 1.
DR Bgee; ENSDARG00000070452; Expressed in granulocyte and 26 other tissues.
DR ExpressionAtlas; F1QPC3; baseline and differential.
DR GO; GO:0140268; C:endoplasmic reticulum-plasma membrane contact site; ISS:UniProtKB.
DR GO; GO:0030176; C:integral component of endoplasmic reticulum membrane; ISS:UniProtKB.
DR GO; GO:0006816; P:calcium ion transport; IEA:UniProtKB-KW.
DR GO; GO:2001256; P:regulation of store-operated calcium entry; ISS:UniProtKB.
DR InterPro; IPR009567; SARAF.
DR PANTHER; PTHR15929; PTHR15929; 1.
DR Pfam; PF06682; SARAF; 1.
PE 2: Evidence at transcript level;
KW Calcium; Calcium transport; Endoplasmic reticulum; Ion transport; Membrane;
KW Reference proteome; Signal; Transmembrane; Transmembrane helix; Transport.
FT SIGNAL 1..21
FT /evidence="ECO:0000255"
FT CHAIN 22..345
FT /note="Store-operated calcium entry-associated regulatory
FT factor"
FT /id="PRO_0000417516"
FT TOPO_DOM 22..183
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT TRANSMEM 184..204
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 205..345
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT REGION 155..178
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 223..289
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 302..345
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 253..275
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 315..345
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 65
FT /note="S -> F (in Ref. 2; AAI24303)"
FT /evidence="ECO:0000305"
FT CONFLICT 165
FT /note="D -> G (in Ref. 2; AAI24303)"
FT /evidence="ECO:0000305"
FT CONFLICT 210
FT /note="N -> H (in Ref. 2; AAI24303)"
FT /evidence="ECO:0000305"
FT CONFLICT 276
FT /note="R -> G (in Ref. 2; AAI24303)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 345 AA; 36742 MW; FD59106277B3594C CRC64;
MRVSGALFLQ FLLLVVPHIS CWNDEAVLLR DVQVLTLYRG RYTTARRSSP VPQLQCIGGS
AGCGSFTPEV VQCYNRGSDG IDAQWECKAD MDNLYRFGRV EVSCEGYNSP NDAYVLRGSC
GLEYTLELTA EGKQQQGSSG FSGFASNFFQ GFSNQKQQQR GGGGDGGGPY QQHSYQNSPS
GDGVGGLLVV AFLLLVAFVV YKMFLCSPTN GHQGFGGDQG YDSTNGAWDN SQGMGPPPPG
FKPDYYSGDH SNSRPGYGFS DSYTRPQSSW TGFTGRTRPG GGGGGGGGFW TGMGTGGILG
YLFGNQRRQP PMGFAGPTYN SNPSPAPKTS SGTRTASGFG TTKRR
