ID   SARAF_DICDI             Reviewed;         320 AA.
AC   Q54P59;
DT   08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT   24-MAY-2005, sequence version 1.
DT   25-MAY-2022, entry version 65.
DE   RecName: Full=Store-operated calcium entry-associated regulatory factor;
DE            Short=SARAF;
DE            Short=SOCE-associated regulatory factor;
DE   AltName: Full=Transmembrane protein 66;
DE   Flags: Precursor;
GN   Name=saraf; Synonyms=tmem66; ORFNames=DDB_G0284777;
OS   Dictyostelium discoideum (Slime mold).
OC   Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC   Dictyosteliaceae; Dictyostelium.
OX   NCBI_TaxID=44689;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX4;
RX   PubMed=15875012; DOI=10.1038/nature03481;
RA   Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA   Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA   Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA   Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA   Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA   Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA   Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA   Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA   Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA   Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA   Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA   Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA   Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA   Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA   Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA   Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA   Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT   "The genome of the social amoeba Dictyostelium discoideum.";
RL   Nature 435:43-57(2005).
CC   -!- FUNCTION: Negative regulator of store-operated Ca(2+) entry (SOCE)
CC       involved in protecting cells from Ca(2+) overfilling. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane {ECO:0000250};
CC       Single-pass type I membrane protein {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the SARAF family. {ECO:0000305}.
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DR   EMBL; AAFI02000071; EAL65041.1; -; Genomic_DNA.
DR   RefSeq; XP_638399.1; XM_633307.1.
DR   AlphaFoldDB; Q54P59; -.
DR   SMR; Q54P59; -.
DR   STRING; 44689.DDB0234076; -.
DR   PaxDb; Q54P59; -.
DR   EnsemblProtists; EAL65041; EAL65041; DDB_G0284777.
DR   GeneID; 8624768; -.
DR   KEGG; ddi:DDB_G0284777; -.
DR   dictyBase; DDB_G0284777; -.
DR   eggNOG; ENOG502QT6Y; Eukaryota.
DR   HOGENOM; CLU_046802_1_1_1; -.
DR   InParanoid; Q54P59; -.
DR   OMA; PIKQIEC; -.
DR   PRO; PR:Q54P59; -.
DR   Proteomes; UP000002195; Chromosome 4.
DR   GO; GO:0030176; C:integral component of endoplasmic reticulum membrane; IBA:GO_Central.
DR   GO; GO:0006816; P:calcium ion transport; IEA:UniProtKB-KW.
DR   GO; GO:2001256; P:regulation of store-operated calcium entry; IBA:GO_Central.
DR   InterPro; IPR009567; SARAF.
DR   PANTHER; PTHR15929; PTHR15929; 1.
DR   Pfam; PF06682; SARAF; 1.
PE   3: Inferred from homology;
KW   Calcium; Calcium transport; Endoplasmic reticulum; Ion transport; Membrane;
KW   Reference proteome; Signal; Transmembrane; Transmembrane helix; Transport.
FT   SIGNAL          1..25
FT                   /evidence="ECO:0000255"
FT   CHAIN           26..320
FT                   /note="Store-operated calcium entry-associated regulatory
FT                   factor"
FT                   /id="PRO_0000328115"
FT   TOPO_DOM        26..157
FT                   /note="Lumenal"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        158..178
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        179..320
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   REGION          186..231
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          285..320
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   320 AA;  35043 MW;  A4CDC829565B1BCA CRC64;
     MKHNYYILFL ILISSLFLSN KVVESYGHRN NNKGGDSKIL LKDVQVLTLR SGEMTKARRS
     SPIQQMECIG GTATKELELY PKTIQCYNMG SNGVDVQWKC EATLDSTVRL GTIDVSCEGY
     SYPEDPYITA DSCGVFYQLE YTNEQRRKAA LAEEETSWVQ IIFCIAFIGL ILYAVFNWCG
     SPQGDDDSNT AQNNTATDNN YPGGFPGNNN NNNNNNNNSN NNNYGGNYQG YPNLNNNNNA
     GGCAQPNNGG GGGGGWFQPG LWTGLASGYL FGRIHSNRNH HHYYNPSPSY NRPRSSYSSG
     SSGGSGISSS SSSYSGTSRR