ID   SARAF_PONAB             Reviewed;         339 AA.
AC   Q5R491;
DT   10-JAN-2006, integrated into UniProtKB/Swiss-Prot.
DT   10-JAN-2006, sequence version 2.
DT   25-MAY-2022, entry version 57.
DE   RecName: Full=Store-operated calcium entry-associated regulatory factor;
DE            Short=SARAF;
DE            Short=SOCE-associated regulatory factor;
DE   AltName: Full=Transmembrane protein 66;
DE   Flags: Precursor;
GN   Name=SARAF; Synonyms=TMEM66;
OS   Pongo abelii (Sumatran orangutan) (Pongo pygmaeus abelii).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Pongo.
OX   NCBI_TaxID=9601;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Brain cortex;
RG   The German cDNA consortium;
RL   Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Negative regulator of store-operated Ca(2+) entry (SOCE)
CC       involved in protecting cells from Ca(2+) overfilling. In response to
CC       cytosolic Ca(2+) elevation after endoplasmic reticulum Ca(2+)
CC       refilling, promotes a slow inactivation of STIM (STIM1 or STIM2)-
CC       dependent SOCE activity: possibly act by facilitating the
CC       deoligomerization of STIM to efficiently turn off ORAI when the
CC       endoplasmic reticulum lumen is filled with the appropriate Ca(2+)
CC       levels, and thus preventing the overload of the cell with excessive
CC       Ca(2+) ions (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Interacts with STIM1; the interaction is inhibit by th
CC       interaction of STIM1 with EFHB. {ECO:0000250|UniProtKB:Q96BY9}.
CC   -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane {ECO:0000250};
CC       Single-pass type I membrane protein {ECO:0000250}. Note=Translocates to
CC       the endoplasmic reticulum-plasma membrane (ER-PM) region in a STIM1-
CC       dependent manner following cytosolic Ca(2+) elevation. {ECO:0000250}.
CC   -!- DOMAIN: The cytoplasmic C-terminal region mediates interaction with
CC       STIM1, while the N-terminal lumenal region mediates regulation of SOCE
CC       activity. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the SARAF family. {ECO:0000305}.
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DR   EMBL; CR861365; CAH93425.1; -; mRNA.
DR   RefSeq; NP_001127007.1; NM_001133535.1.
DR   AlphaFoldDB; Q5R491; -.
DR   SMR; Q5R491; -.
DR   STRING; 9601.ENSPPYP00000020718; -.
DR   GeneID; 100174030; -.
DR   KEGG; pon:100174030; -.
DR   CTD; 51669; -.
DR   eggNOG; ENOG502QT6Y; Eukaryota.
DR   InParanoid; Q5R491; -.
DR   Proteomes; UP000001595; Unplaced.
DR   GO; GO:0140268; C:endoplasmic reticulum-plasma membrane contact site; ISS:UniProtKB.
DR   GO; GO:0030176; C:integral component of endoplasmic reticulum membrane; ISS:UniProtKB.
DR   GO; GO:0006816; P:calcium ion transport; IEA:UniProtKB-KW.
DR   GO; GO:2001256; P:regulation of store-operated calcium entry; ISS:UniProtKB.
DR   InterPro; IPR009567; SARAF.
DR   PANTHER; PTHR15929; PTHR15929; 1.
DR   Pfam; PF06682; SARAF; 1.
PE   2: Evidence at transcript level;
KW   Calcium; Calcium transport; Endoplasmic reticulum; Ion transport; Membrane;
KW   Reference proteome; Signal; Transmembrane; Transmembrane helix; Transport.
FT   SIGNAL          1..30
FT                   /evidence="ECO:0000255"
FT   CHAIN           31..339
FT                   /note="Store-operated calcium entry-associated regulatory
FT                   factor"
FT                   /id="PRO_0000045487"
FT   TOPO_DOM        31..173
FT                   /note="Lumenal"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        174..194
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        195..339
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   REGION          313..339
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        313..332
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   339 AA;  36993 MW;  3E2E34B021BA698E CRC64;
     MASACGPGAA GHCLLLGLHL FLLTAGPALG WNDPECMLLR DVKALTLHYD RYTTSRRLDP
     IPQLKCVGGT AGCDSYTPKV IQCQNKGWDG YDVQWECKTD LDIAYKFGKT VVSCEGYESS
     EDQYVLRGSC GLEYNLDYTE LGLQKLKESG KQHGFASFSD YYYKWYSADS CNMSGLITIV
     VLLGIAFVVY KLFLSDGQYS PPPYSEYPPF SHRYQRFTNS AGPPPPGFKS EFTGPQNTGH
     GATSGFGSAF TGQQGYENSG PGFWTGLGTG GILGYLFGSN RAATPFSDSW YYPSYPPSYP
     GTWNRAYSPL RGGSGSYSAC SNSDTKTRTA SGYGGTRRR