ID   SARAF_XENLA             Reviewed;         303 AA.
AC   Q0IHF9;
DT   16-MAY-2012, integrated into UniProtKB/Swiss-Prot.
DT   16-MAY-2012, sequence version 2.
DT   03-AUG-2022, entry version 42.
DE   RecName: Full=Store-operated calcium entry-associated regulatory factor;
DE            Short=SARAF;
DE            Short=SOCE-associated regulatory factor;
DE   AltName: Full=Transmembrane protein 66;
DE   Flags: Precursor;
GN   Name=saraf; Synonyms=tmem66;
OS   Xenopus laevis (African clawed frog).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Xenopus.
OX   NCBI_TaxID=8355;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Fat body;
RG   NIH - Xenopus Gene Collection (XGC) project;
RL   Submitted (SEP-2006) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Negative regulator of store-operated Ca(2+) entry (SOCE)
CC       involved in protecting cells from Ca(2+) overfilling. In response to
CC       cytosolic Ca(2+) elevation after endoplasmic reticulum Ca(2+)
CC       refilling, promotes a slow inactivation of STIM (stim1 or stim2)-
CC       dependent SOCE activity (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane {ECO:0000250};
CC       Single-pass type I membrane protein {ECO:0000250}. Note=Translocates to
CC       the endoplasmic reticulum-plasma membrane (ER-PM) region in a STIM1-
CC       dependent manner following cytosolic Ca(2+) elevation. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the SARAF family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAI23171.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR   EMBL; BC123170; AAI23171.1; ALT_INIT; mRNA.
DR   RefSeq; NP_001090323.1; NM_001096854.1.
DR   AlphaFoldDB; Q0IHF9; -.
DR   SMR; Q0IHF9; -.
DR   DNASU; 779232; -.
DR   GeneID; 779232; -.
DR   KEGG; xla:779232; -.
DR   CTD; 779232; -.
DR   Xenbase; XB-GENE-997093; saraf.S.
DR   OrthoDB; 1402326at2759; -.
DR   Proteomes; UP000186698; Chromosome 1S.
DR   Bgee; 779232; Expressed in blastula and 19 other tissues.
DR   GO; GO:0140268; C:endoplasmic reticulum-plasma membrane contact site; ISS:UniProtKB.
DR   GO; GO:0030176; C:integral component of endoplasmic reticulum membrane; ISS:UniProtKB.
DR   GO; GO:0006816; P:calcium ion transport; IEA:UniProtKB-KW.
DR   GO; GO:2001256; P:regulation of store-operated calcium entry; ISS:UniProtKB.
DR   InterPro; IPR009567; SARAF.
DR   PANTHER; PTHR15929; PTHR15929; 1.
DR   Pfam; PF06682; SARAF; 1.
PE   2: Evidence at transcript level;
KW   Calcium; Calcium transport; Endoplasmic reticulum; Ion transport; Membrane;
KW   Reference proteome; Signal; Transmembrane; Transmembrane helix; Transport.
FT   SIGNAL          1..23
FT                   /evidence="ECO:0000255"
FT   CHAIN           24..303
FT                   /note="Store-operated calcium entry-associated regulatory
FT                   factor"
FT                   /id="PRO_0000417517"
FT   TOPO_DOM        24..168
FT                   /note="Lumenal"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        169..189
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        190..303
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   REGION          195..224
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          265..303
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        265..295
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   303 AA;  33824 MW;  7E25E5AF0E91D649 CRC64;
     MTNMWTLLLL PFFAMLQVHY AWCWSPQDRV LLRDIQTITL YADRYTNARR SAPVPQLKCI
     GGNAGCHAMV PQVVQCHNRG WDGLDVQWEC RVDMDNSYRF GKVEVSCEGF DFPEDPYVLR
     GSCGLEYTLE LTEEGRKRTQ DGYRSAGFGS GYFQSNSQSW DSKTDGSSAI VLIFIVILAY
     GVYKLFLSGP SVQQHPRPDE YGYDHQSQTH SSAPPPGFKS DFTGFSSGYG TGHVNQGPGF
     WTGLGTGGVL GYLFGNRRAQ PYQSPYFNTW TGPSHSESMH QNDRPPQSSG VRTASGFGGT
     KRR