ID   SARAF_XENTR             Reviewed;         319 AA.
AC   F7C1E2;
DT   16-MAY-2012, integrated into UniProtKB/Swiss-Prot.
DT   16-MAY-2012, sequence version 2.
DT   03-AUG-2022, entry version 36.
DE   RecName: Full=Store-operated calcium entry-associated regulatory factor;
DE            Short=SARAF;
DE            Short=SOCE-associated regulatory factor;
DE   AltName: Full=Transmembrane protein 66;
DE   Flags: Precursor;
GN   Name=saraf; Synonyms=tmem66;
OS   Xenopus tropicalis (Western clawed frog) (Silurana tropicalis).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Silurana.
OX   NCBI_TaxID=8364;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=20431018; DOI=10.1126/science.1183670;
RA   Hellsten U., Harland R.M., Gilchrist M.J., Hendrix D., Jurka J.,
RA   Kapitonov V., Ovcharenko I., Putnam N.H., Shu S., Taher L., Blitz I.L.,
RA   Blumberg B., Dichmann D.S., Dubchak I., Amaya E., Detter J.C., Fletcher R.,
RA   Gerhard D.S., Goodstein D., Graves T., Grigoriev I.V., Grimwood J.,
RA   Kawashima T., Lindquist E., Lucas S.M., Mead P.E., Mitros T., Ogino H.,
RA   Ohta Y., Poliakov A.V., Pollet N., Robert J., Salamov A., Sater A.K.,
RA   Schmutz J., Terry A., Vize P.D., Warren W.C., Wells D., Wills A.,
RA   Wilson R.K., Zimmerman L.B., Zorn A.M., Grainger R., Grammer T.,
RA   Khokha M.K., Richardson P.M., Rokhsar D.S.;
RT   "The genome of the Western clawed frog Xenopus tropicalis.";
RL   Science 328:633-636(2010).
CC   -!- FUNCTION: Negative regulator of store-operated Ca(2+) entry (SOCE)
CC       involved in protecting cells from Ca(2+) overfilling. In response to
CC       cytosolic Ca(2+) elevation after endoplasmic reticulum Ca(2+)
CC       refilling, promotes a slow inactivation of STIM (stim1 or stim2)-
CC       dependent SOCE activity (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane {ECO:0000250};
CC       Single-pass type I membrane protein {ECO:0000250}. Note=Translocates to
CC       the endoplasmic reticulum-plasma membrane (ER-PM) region in a STIM1-
CC       dependent manner following cytosolic Ca(2+) elevation. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the SARAF family. {ECO:0000305}.
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DR   EMBL; AAMC01129898; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   AlphaFoldDB; F7C1E2; -.
DR   SMR; F7C1E2; -.
DR   STRING; 8364.ENSXETP00000055286; -.
DR   PaxDb; F7C1E2; -.
DR   eggNOG; ENOG502QT6Y; Eukaryota.
DR   HOGENOM; CLU_046802_0_1_1; -.
DR   InParanoid; F7C1E2; -.
DR   Proteomes; UP000008143; Genome assembly.
DR   Proteomes; UP000790000; Unplaced.
DR   GO; GO:0140268; C:endoplasmic reticulum-plasma membrane contact site; ISS:UniProtKB.
DR   GO; GO:0030176; C:integral component of endoplasmic reticulum membrane; ISS:UniProtKB.
DR   GO; GO:0006816; P:calcium ion transport; IEA:UniProtKB-KW.
DR   GO; GO:2001256; P:regulation of store-operated calcium entry; ISS:UniProtKB.
DR   InterPro; IPR009567; SARAF.
DR   PANTHER; PTHR15929; PTHR15929; 1.
DR   Pfam; PF06682; SARAF; 1.
PE   3: Inferred from homology;
KW   Calcium; Calcium transport; Endoplasmic reticulum; Ion transport; Membrane;
KW   Reference proteome; Signal; Transmembrane; Transmembrane helix; Transport.
FT   SIGNAL          1..26
FT                   /evidence="ECO:0000255"
FT   CHAIN           27..319
FT                   /note="Store-operated calcium entry-associated regulatory
FT                   factor"
FT                   /id="PRO_0000417518"
FT   TOPO_DOM        27..171
FT                   /note="Lumenal"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        172..192
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        193..319
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   REGION          200..221
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          292..319
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        292..311
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   319 AA;  35340 MW;  B0908D6577C81C1E CRC64;
     MVEVANMWSL LLLPLLALLQ IPGAWCWNHQ ERVLLRDIQA ITLYADRYTN ARRSAPVPQL
     KCIGGSAGCH TMVPQVVQCH NRGWDGFDVQ WECKVDMDNS YRFGKVEVSC EGFDYPEDPY
     VLRGSCGLEY TLELTEEGRK RSQDGYRSGG FGSGYFQSNS NSWDTKTNGS SAIVLIVIVI
     IAYGVYKLFL SGPSVQEHPR PDEYGYDFQS QTRASAPPPP GFKSDFTDPH LCKIFGASKS
     FSSGYGTSHS NQGPGFWTGL GTGGVLGYLF GNRRAQPYQA PYFNTWTGPS NSDSMHQNYR
     PPQSSGVRTA SGFGGTKRR