SARD1_ARATH
ID SARD1_ARATH Reviewed; 451 AA.
AC Q9C9T2; Q93Z50;
DT 27-MAY-2015, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 03-AUG-2022, entry version 98.
DE RecName: Full=Protein SAR DEFICIENT 1 {ECO:0000303|PubMed:20921422};
GN Name=SARD1 {ECO:0000303|PubMed:20921422};
GN OrderedLocusNames=At1g73805 {ECO:0000312|Araport:AT1G73805};
GN ORFNames=F25P22.22 {ECO:0000312|EMBL:AAG52065.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702 {ECO:0000312|Proteomes:UP000006548};
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 242-451.
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP FUNCTION, DISRUPTION PHENOTYPE, INDUCTION BY PSEUDOMONAS SYRINGAE,
RP SUBCELLULAR LOCATION, AND DNA-BINDING.
RC STRAIN=cv. Columbia;
RX PubMed=20921422; DOI=10.1073/pnas.1005225107;
RA Zhang Y., Xu S., Ding P., Wang D., Cheng Y.T., He J., Gao M., Xu F., Li Y.,
RA Zhu Z., Li X., Zhang Y.;
RT "Control of salicylic acid synthesis and systemic acquired resistance by
RT two members of a plant-specific family of transcription factors.";
RL Proc. Natl. Acad. Sci. U.S.A. 107:18220-18225(2010).
RN [5]
RP FUNCTION, DISRUPTION PHENOTYPE, AND INDUCTION BY PSEUDOMONAS SYRINGAE AND
RP FLG22.
RC STRAIN=cv. Columbia;
RX PubMed=21615571; DOI=10.1111/j.1365-313x.2011.04655.x;
RA Wang L., Tsuda K., Truman W., Sato M., Nguyen L.V., Katagiri F.,
RA Glazebrook J.;
RT "CBP60g and SARD1 play partially redundant critical roles in salicylic acid
RT signaling.";
RL Plant J. 67:1029-1041(2011).
RN [6]
RP FUNCTION IN TRANSCRIPTION REGULATION, AND INDUCTION BY FLG22.
RX PubMed=23153277; DOI=10.1186/1471-2229-12-216;
RA Truman W., Glazebrook J.;
RT "Co-expression analysis identifies putative targets for CBP60g and SARD1
RT regulation.";
RL BMC Plant Biol. 12:216-216(2012).
RN [7]
RP INTERACTION WITH V.DAHLIAE SCP41.
RX PubMed=29757140; DOI=10.7554/elife.34902;
RA Qin J., Wang K., Sun L., Xing H., Wang S., Li L., Chen S., Guo H.S.,
RA Zhang J.;
RT "The plant-specific transcription factors CBP60g and SARD1 are targeted by
RT a Verticillium secretory protein VdSCP41 to modulate immunity.";
RL Elife 7:e34902-e34902(2018).
CC -!- FUNCTION: Transcription activator that binds DNA in a sequence-specific
CC manner, 5'-GAAATTTTGG-3', to promote the expression of target genes
CC (PubMed:20921422, PubMed:21615571, PubMed:23153277). Recruited to the
CC promoter of ICS1 and other defense-related genes (e.g. PR1 and SID2) in
CC response to both biotic (e.g. Pseudomonas syringae pv. maculicola
CC ES4326) and abiotic stresses (e.g. UV-B), thus triggering slow defense
CC responses by stimulating salicylic acid (SA) biosynthesis. Required for
CC basal and systemic acquired resistance to P. syringae pv. maculicola
CC and Hyaloperonospora arabidopsidis (PubMed:20921422, PubMed:21615571).
CC {ECO:0000269|PubMed:20921422, ECO:0000269|PubMed:21615571,
CC ECO:0000269|PubMed:23153277}.
CC -!- SUBUNIT: (Microbial infection) Interacts with V.dahliae SCP41.
CC {ECO:0000269|PubMed:29757140}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:20921422}.
CC -!- INDUCTION: Induced slowly by Pseudomonas syringae p.v. maculicola
CC ES4326 and by microbe-associated molecular patterns (MAMPs) such as
CC flg22 in both local and systemic leaves. {ECO:0000269|PubMed:20921422,
CC ECO:0000269|PubMed:21615571, ECO:0000269|PubMed:23153277}.
CC -!- DISRUPTION PHENOTYPE: Compromised basal resistance and systemic
CC acquired resistance (SAR) induced by Pseudomonas syringae p.v.
CC maculicola ES4326 toward Hyaloperonospora arabidopsidis Noco2. Plants
CC lacking both SARD1 and CBP60G fail to accumulate salicylic acid (SA)
CC and to express PR1 and SID2 upon both biotic and abiotic stresses.
CC {ECO:0000269|PubMed:20921422, ECO:0000269|PubMed:21615571}.
CC -!- SIMILARITY: Belongs to the plant ACBP60 protein family.
CC {ECO:0000305|PubMed:20921422}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAL25542.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR EMBL; AC012679; AAG52065.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE35510.1; -; Genomic_DNA.
DR EMBL; AY058126; AAL25542.1; ALT_INIT; mRNA.
DR EMBL; BT023740; AAZ23932.1; -; mRNA.
DR PIR; D96765; D96765.
DR RefSeq; NP_565074.2; NM_106040.3.
DR AlphaFoldDB; Q9C9T2; -.
DR STRING; 3702.AT1G73805.1; -.
DR PaxDb; Q9C9T2; -.
DR PRIDE; Q9C9T2; -.
DR ProteomicsDB; 232839; -.
DR EnsemblPlants; AT1G73805.1; AT1G73805.1; AT1G73805.
DR GeneID; 843716; -.
DR Gramene; AT1G73805.1; AT1G73805.1; AT1G73805.
DR KEGG; ath:AT1G73805; -.
DR Araport; AT1G73805; -.
DR TAIR; locus:505006222; AT1G73805.
DR eggNOG; ENOG502QR87; Eukaryota.
DR HOGENOM; CLU_031504_5_0_1; -.
DR InParanoid; Q9C9T2; -.
DR OMA; NITEKRM; -.
DR OrthoDB; 546629at2759; -.
DR PhylomeDB; Q9C9T2; -.
DR PRO; PR:Q9C9T2; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q9C9T2; baseline and differential.
DR Genevisible; Q9C9T2; AT.
DR GO; GO:0005634; C:nucleus; IDA:TAIR.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IDA:UniProtKB.
DR GO; GO:0043565; F:sequence-specific DNA binding; IDA:UniProtKB.
DR GO; GO:0071219; P:cellular response to molecule of bacterial origin; IDA:UniProtKB.
DR GO; GO:0042742; P:defense response to bacterium; IMP:UniProtKB.
DR GO; GO:0002229; P:defense response to oomycetes; IMP:UniProtKB.
DR GO; GO:0009626; P:plant-type hypersensitive response; IEA:UniProtKB-KW.
DR GO; GO:1900426; P:positive regulation of defense response to bacterium; IMP:UniProtKB.
DR GO; GO:0080142; P:regulation of salicylic acid biosynthetic process; IMP:UniProtKB.
DR GO; GO:0010112; P:regulation of systemic acquired resistance; IGI:TAIR.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IDA:UniProtKB.
DR GO; GO:0009617; P:response to bacterium; IDA:UniProtKB.
DR GO; GO:0010224; P:response to UV-B; IDA:UniProtKB.
DR InterPro; IPR012416; CBP60.
DR PANTHER; PTHR31713; PTHR31713; 1.
DR Pfam; PF07887; Calmodulin_bind; 1.
PE 1: Evidence at protein level;
KW Activator; DNA-binding; Hypersensitive response; Nucleus; Plant defense;
KW Reference proteome; Transcription; Transcription regulation.
FT CHAIN 1..451
FT /note="Protein SAR DEFICIENT 1"
FT /id="PRO_0000433044"
FT REGION 149..270
FT /note="DNA-binding"
FT /evidence="ECO:0000303|PubMed:20921422"
SQ SEQUENCE 451 AA; 50705 MW; 839B84EF5AF204B8 CRC64;
MAGKRLFQDL DSDQENKSEK RIKSVLPSLA SPISSVFGAL ISENTLRSVL EPVIRKVVRQ
EVEYGISKRF RLSRSSSFRI EAPEATTPTL KLIFRKNLMT PIFTGSKISD VDNNPLEIIL
VDDSNKPVNL NRPIKLDIVA LHGDFPSGDK WTSDEFESNI IKERDGKRPL LAGEVSVTVR
NGVATIGEIV FTDNSSWIRS RKFRIGAKVA KGSSGQGVVV CEAMTEAIVV RDHRGELYKK
HHPPMLEDEV WRLEKIGKDG AFHKKLSSRH INTVQDFLKL SVVDVDELRQ ILGPGMSDRK
WEVTLKHARE CILGNKLYIS RGPNFFMILN PICEVMKALI DGHVLSSQES LNQPYVKNLV
RDAYSKGNFL EVGERTANEA ALLTQGDDLD QQYAASHYQN IEIDKSYQQN GYVQERSTNN
LEIVNEGYIT TPAEFNICFT GSSSQNHINP F
