ID   SARS_STAA1              Reviewed;         250 AA.
AC   P0C0R2; A7WX61; Q9KWJ2;
DT   22-NOV-2005, integrated into UniProtKB/Swiss-Prot.
DT   22-NOV-2005, sequence version 1.
DT   25-MAY-2022, entry version 94.
DE   RecName: Full=HTH-type transcriptional regulator SarS;
DE   AltName: Full=Staphylococcal accessory regulator S;
GN   Name=sarS; Synonyms=sarH1; OrderedLocusNames=SAHV_0111;
OS   Staphylococcus aureus (strain Mu3 / ATCC 700698).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC   Staphylococcus.
OX   NCBI_TaxID=418127;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=10708580; DOI=10.1006/bbrc.2000.2277;
RA   Kuroda M., Kuwahara-Arai K., Hiramatsu K.;
RT   "Identification of the up- and down-regulated genes in vancomycin-resistant
RT   Staphylococcus aureus strains Mu3 and Mu50 by cDNA differential
RT   hybridization method.";
RL   Biochem. Biophys. Res. Commun. 269:485-490(2000).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Mu3 / ATCC 700698;
RX   PubMed=17954695; DOI=10.1128/aac.00534-07;
RA   Neoh H.-M., Cui L., Yuzawa H., Takeuchi F., Matsuo M., Hiramatsu K.;
RT   "Mutated response regulator graR is responsible for phenotypic conversion
RT   of Staphylococcus aureus from heterogeneous vancomycin-intermediate
RT   resistance to vancomycin-intermediate resistance.";
RL   Antimicrob. Agents Chemother. 52:45-53(2008).
CC   -!- FUNCTION: Transcriptional regulator that controls expression of some
CC       virulence factors in a cell density-dependent manner. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the SarA family. {ECO:0000305}.
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DR   EMBL; AB035454; BAB03341.1; -; Genomic_DNA.
DR   EMBL; AP009324; BAF76994.1; -; Genomic_DNA.
DR   RefSeq; WP_000876756.1; NC_009782.1.
DR   PDB; 1P4X; X-ray; 2.20 A; A=1-250.
DR   PDBsum; 1P4X; -.
DR   AlphaFoldDB; P0C0R2; -.
DR   SMR; P0C0R2; -.
DR   KEGG; saw:SAHV_0111; -.
DR   HOGENOM; CLU_097164_0_0_9; -.
DR   OMA; DHIIKQF; -.
DR   EvolutionaryTrace; P0C0R2; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:InterPro.
DR   Gene3D; 1.10.10.10; -; 2.
DR   InterPro; IPR000835; HTH_MarR-typ.
DR   InterPro; IPR010166; SarA/Rot.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   InterPro; IPR036390; WH_DNA-bd_sf.
DR   SMART; SM00347; HTH_MARR; 2.
DR   SUPFAM; SSF46785; SSF46785; 2.
DR   TIGRFAMs; TIGR01889; Staph_reg_Sar; 2.
PE   1: Evidence at protein level;
KW   3D-structure; Activator; Cytoplasm; DNA-binding; Repeat; Repressor;
KW   Transcription; Transcription regulation; Virulence.
FT   CHAIN           1..250
FT                   /note="HTH-type transcriptional regulator SarS"
FT                   /id="PRO_0000219590"
FT   DNA_BIND        53..76
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255"
FT   DNA_BIND        177..200
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   250 AA;  29890 MW;  4680D50FE86DDC19 CRC64;
     MKYNNHDKIR DFIIIEAYMF RFKKKVKPEV DMTIKEFILL TYLFHQQENT LPFKKIVSDL
     CYKQSDLVQH IKVLVKHSYI SKVRSKIDER NTYISISEEQ REKIAERVTL FDQIIKQFNL
     ADQSESQMIP KDSKEFLNLM MYTMYFKNII KKHLTLSFVE FTILAIITSQ NKNIVLLKDL
     IETIHHKYPQ TVRALNNLKK QGYLIKERST EDERKILIHM DDAQQDHAEQ LLAQVNQLLA
     DKDHLHLVFE