ID   SARS_STAAC              Reviewed;         250 AA.
AC   Q5HJQ7;
DT   22-NOV-2005, integrated into UniProtKB/Swiss-Prot.
DT   15-FEB-2005, sequence version 1.
DT   25-MAY-2022, entry version 95.
DE   RecName: Full=HTH-type transcriptional regulator SarS;
DE   AltName: Full=Staphylococcal accessory regulator S;
GN   Name=sarS; Synonyms=sarH1; OrderedLocusNames=SACOL0096;
OS   Staphylococcus aureus (strain COL).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC   Staphylococcus.
OX   NCBI_TaxID=93062;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=COL;
RX   PubMed=15774886; DOI=10.1128/jb.187.7.2426-2438.2005;
RA   Gill S.R., Fouts D.E., Archer G.L., Mongodin E.F., DeBoy R.T., Ravel J.,
RA   Paulsen I.T., Kolonay J.F., Brinkac L.M., Beanan M.J., Dodson R.J.,
RA   Daugherty S.C., Madupu R., Angiuoli S.V., Durkin A.S., Haft D.H.,
RA   Vamathevan J.J., Khouri H., Utterback T.R., Lee C., Dimitrov G., Jiang L.,
RA   Qin H., Weidman J., Tran K., Kang K.H., Hance I.R., Nelson K.E.,
RA   Fraser C.M.;
RT   "Insights on evolution of virulence and resistance from the complete genome
RT   analysis of an early methicillin-resistant Staphylococcus aureus strain and
RT   a biofilm-producing methicillin-resistant Staphylococcus epidermidis
RT   strain.";
RL   J. Bacteriol. 187:2426-2438(2005).
RN   [2]
RP   FUNCTION, GROWTH PHASE-DEPENDENT EXPRESSION, AND REGULATION BY TCAR.
RX   PubMed=15126456; DOI=10.1128/jb.186.10.2966-2972.2004;
RA   McCallum N., Bischoff M., Maki H., Wada A., Berger-Baechi B.;
RT   "TcaR, a putative MarR-like regulator of sarS expression.";
RL   J. Bacteriol. 186:2966-2972(2004).
CC   -!- FUNCTION: Transcriptional regulator that controls expression of some
CC       virulence factors in a cell density-dependent manner (By similarity).
CC       Acts as a transcriptional activator of the gene encoding protein A
CC       (spa). {ECO:0000250, ECO:0000269|PubMed:15126456}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC   -!- INDUCTION: Expressed at the early exponential growth phase, decreases
CC       through exponential phase and reaches a steady-state level at post-
CC       exponential phase. Activated by TcaR.
CC   -!- SIMILARITY: Belongs to the SarA family. {ECO:0000305}.
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DR   EMBL; CP000046; AAW38739.1; -; Genomic_DNA.
DR   RefSeq; WP_000876756.1; NC_002951.2.
DR   AlphaFoldDB; Q5HJQ7; -.
DR   SMR; Q5HJQ7; -.
DR   EnsemblBacteria; AAW38739; AAW38739; SACOL0096.
DR   KEGG; sac:SACOL0096; -.
DR   HOGENOM; CLU_097164_0_0_9; -.
DR   OMA; DHIIKQF; -.
DR   Proteomes; UP000000530; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:InterPro.
DR   Gene3D; 1.10.10.10; -; 2.
DR   InterPro; IPR000835; HTH_MarR-typ.
DR   InterPro; IPR010166; SarA/Rot.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   InterPro; IPR036390; WH_DNA-bd_sf.
DR   SMART; SM00347; HTH_MARR; 2.
DR   SUPFAM; SSF46785; SSF46785; 2.
DR   TIGRFAMs; TIGR01889; Staph_reg_Sar; 2.
PE   2: Evidence at transcript level;
KW   Activator; Cytoplasm; DNA-binding; Repeat; Repressor; Transcription;
KW   Transcription regulation; Virulence.
FT   CHAIN           1..250
FT                   /note="HTH-type transcriptional regulator SarS"
FT                   /id="PRO_0000219591"
FT   DNA_BIND        53..76
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255"
FT   DNA_BIND        177..200
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   250 AA;  29890 MW;  4680D50FE86DDC19 CRC64;
     MKYNNHDKIR DFIIIEAYMF RFKKKVKPEV DMTIKEFILL TYLFHQQENT LPFKKIVSDL
     CYKQSDLVQH IKVLVKHSYI SKVRSKIDER NTYISISEEQ REKIAERVTL FDQIIKQFNL
     ADQSESQMIP KDSKEFLNLM MYTMYFKNII KKHLTLSFVE FTILAIITSQ NKNIVLLKDL
     IETIHHKYPQ TVRALNNLKK QGYLIKERST EDERKILIHM DDAQQDHAEQ LLAQVNQLLA
     DKDHLHLVFE