ID   SARX_STAA8              Reviewed;         119 AA.
AC   Q2G0D1;
DT   31-OCT-2006, integrated into UniProtKB/Swiss-Prot.
DT   31-OCT-2006, sequence version 2.
DT   25-MAY-2022, entry version 90.
DE   RecName: Full=HTH-type transcriptional regulator SarX;
DE   AltName: Full=Staphylococcal accessory regulator X;
GN   Name=sarX; OrderedLocusNames=SAOUHSC_00674;
OS   Staphylococcus aureus (strain NCTC 8325 / PS 47).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC   Staphylococcus.
OX   NCBI_TaxID=93061;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=NCTC 8325 / PS 47;
RA   Gillaspy A.F., Worrell V., Orvis J., Roe B.A., Dyer D.W., Iandolo J.J.;
RT   "The Staphylococcus aureus NCTC 8325 genome.";
RL   (In) Fischetti V., Novick R., Ferretti J., Portnoy D., Rood J. (eds.);
RL   Gram positive pathogens, 2nd edition, pp.381-412, ASM Press, Washington
RL   D.C. (2006).
RN   [2]
RP   FUNCTION, AND INDUCTION.
RX   PubMed=16740935; DOI=10.1128/jb.00297-06;
RA   Manna A.C., Cheung A.L.;
RT   "Expression of SarX, a negative regulator of agr and exoprotein synthesis,
RT   is activated by MgrA in Staphylococcus aureus.";
RL   J. Bacteriol. 188:4288-4299(2006).
CC   -!- FUNCTION: Involved in the regulation of virulence genes. Acts as a
CC       repressor of the agr locus and consequently targets genes regulated by
CC       the agr system such as sspA, hla and hlb. Binds directly to the agr
CC       promoter region. {ECO:0000269|PubMed:16740935}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC   -!- INDUCTION: Transcriptionally activated by MgrA. Expressed maximally
CC       during the stationary phase of growth. {ECO:0000269|PubMed:16740935}.
CC   -!- SIMILARITY: Belongs to the SarA family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=ABD29807.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; CP000253; ABD29807.1; ALT_INIT; Genomic_DNA.
DR   RefSeq; WP_001090985.1; NZ_LS483365.1.
DR   RefSeq; YP_499233.1; NC_007795.1.
DR   PDB; 5HS5; X-ray; 2.55 A; A/B=7-119.
DR   PDB; 5YWJ; X-ray; 2.10 A; A=1-119.
DR   PDB; 6K8E; X-ray; 2.00 A; A/D=1-119.
DR   PDBsum; 5HS5; -.
DR   PDBsum; 5YWJ; -.
DR   PDBsum; 6K8E; -.
DR   AlphaFoldDB; Q2G0D1; -.
DR   SMR; Q2G0D1; -.
DR   STRING; 1280.SAXN108_0735; -.
DR   EnsemblBacteria; ABD29807; ABD29807; SAOUHSC_00674.
DR   GeneID; 3919437; -.
DR   KEGG; sao:SAOUHSC_00674; -.
DR   PATRIC; fig|93061.5.peg.605; -.
DR   eggNOG; ENOG503057T; Bacteria.
DR   HOGENOM; CLU_166896_0_0_9; -.
DR   Proteomes; UP000008816; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0006355; P:regulation of transcription, DNA-templated; IEA:InterPro.
DR   Gene3D; 1.10.10.10; -; 1.
DR   InterPro; IPR010166; SarA/Rot.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   InterPro; IPR036390; WH_DNA-bd_sf.
DR   SUPFAM; SSF46785; SSF46785; 1.
DR   TIGRFAMs; TIGR01889; Staph_reg_Sar; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Cytoplasm; DNA-binding; Reference proteome; Repressor;
KW   Transcription; Transcription regulation; Virulence.
FT   CHAIN           1..119
FT                   /note="HTH-type transcriptional regulator SarX"
FT                   /id="PRO_0000259136"
FT   DNA_BIND        55..78
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255"
FT   HELIX           9..27
FT                   /evidence="ECO:0007829|PDB:6K8E"
FT   HELIX           31..43
FT                   /evidence="ECO:0007829|PDB:6K8E"
FT   STRAND          44..47
FT                   /evidence="ECO:0007829|PDB:6K8E"
FT   HELIX           51..62
FT                   /evidence="ECO:0007829|PDB:6K8E"
FT   HELIX           66..78
FT                   /evidence="ECO:0007829|PDB:6K8E"
FT   STRAND          81..86
FT                   /evidence="ECO:0007829|PDB:6K8E"
FT   STRAND          94..97
FT                   /evidence="ECO:0007829|PDB:6K8E"
FT   HELIX           100..117
FT                   /evidence="ECO:0007829|PDB:6K8E"
SQ   SEQUENCE   119 AA;  14179 MW;  6E0470B6CB2EEB94 CRC64;
     MNTEKLETLL GFYKQYKALS EYIDKKYKLS LNDLAVLDLT MKHCKDEKVL MQSFLKTAMD
     ELDLSRTKLL VSIRRLIEKE RLSKVRSSKD ERKIYIYLNN DDISKFNALF EDVEQFLNI