SARZ_STAA8
ID SARZ_STAA8 Reviewed; 148 AA.
AC Q2FVN3; A0JBY0;
DT 17-APR-2007, integrated into UniProtKB/Swiss-Prot.
DT 21-MAR-2006, sequence version 1.
DT 25-MAY-2022, entry version 103.
DE RecName: Full=HTH-type transcriptional regulator SarZ;
DE AltName: Full=Staphylococcal accessory regulator Z;
GN Name=sarZ; OrderedLocusNames=SAOUHSC_02669;
OS Staphylococcus aureus (strain NCTC 8325 / PS 47).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC Staphylococcus.
OX NCBI_TaxID=93061;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, INDUCTION, AND MUTAGENESIS OF
RP PHE-14; LYS-25; VAL-43; GLY-67; THR-70; VAL-82; ARG-92; ILE-96; LYS-103;
RP GLU-124; LEU-134 AND LYS-140.
RX PubMed=17087772; DOI=10.1111/j.1365-2958.2006.05480.x;
RA Kaito C., Morishita D., Matsumoto Y., Kurokawa K., Sekimizu K.;
RT "Novel DNA binding protein SarZ contributes to virulence in Staphylococcus
RT aureus.";
RL Mol. Microbiol. 62:1601-1617(2006).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=NCTC 8325 / PS 47;
RA Gillaspy A.F., Worrell V., Orvis J., Roe B.A., Dyer D.W., Iandolo J.J.;
RT "The Staphylococcus aureus NCTC 8325 genome.";
RL (In) Fischetti V., Novick R., Ferretti J., Portnoy D., Rood J. (eds.);
RL Gram positive pathogens, 2nd edition, pp.381-412, ASM Press, Washington
RL D.C. (2006).
CC -!- FUNCTION: Activates transcription of virulence factors alpha- and beta
CC hemolysin genes (hla and hlb). Also, activates RNAIII expression, a
CC central regulator transcribed from the agr locus.
CC {ECO:0000269|PubMed:17087772}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC -!- INDUCTION: Transcriptionally activated by CvfA.
CC {ECO:0000269|PubMed:17087772}.
CC -!- SIMILARITY: Belongs to the SarZ family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AB251456; BAF36712.1; -; Genomic_DNA.
DR EMBL; CP000253; ABD31677.1; -; Genomic_DNA.
DR RefSeq; WP_000289213.1; NZ_LS483365.1.
DR RefSeq; YP_501131.1; NC_007795.1.
DR AlphaFoldDB; Q2FVN3; -.
DR SMR; Q2FVN3; -.
DR STRING; 1280.SAXN108_2640; -.
DR EnsemblBacteria; ABD31677; ABD31677; SAOUHSC_02669.
DR GeneID; 3921231; -.
DR KEGG; sao:SAOUHSC_02669; -.
DR PATRIC; fig|93061.5.peg.2415; -.
DR eggNOG; COG1846; Bacteria.
DR HOGENOM; CLU_083287_3_2_9; -.
DR OMA; MMVLWER; -.
DR PRO; PR:Q2FVN3; -.
DR Proteomes; UP000008816; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:InterPro.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IBA:GO_Central.
DR Gene3D; 1.10.10.10; -; 1.
DR InterPro; IPR000835; HTH_MarR-typ.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR InterPro; IPR036390; WH_DNA-bd_sf.
DR Pfam; PF01047; MarR; 1.
DR PRINTS; PR00598; HTHMARR.
DR SMART; SM00347; HTH_MARR; 1.
DR SUPFAM; SSF46785; SSF46785; 1.
DR PROSITE; PS50995; HTH_MARR_2; 1.
PE 1: Evidence at protein level;
KW Activator; Cytoplasm; DNA-binding; Reference proteome; Transcription;
KW Transcription regulation; Virulence.
FT CHAIN 1..148
FT /note="HTH-type transcriptional regulator SarZ"
FT /id="PRO_0000284461"
FT DOMAIN 9..139
FT /note="HTH marR-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00345"
FT DNA_BIND 55..78
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00345"
FT MUTAGEN 14
FT /note="F->A: Reduced DNA binding activity, hemolysin
FT production and virulence in silkworm-infection model."
FT /evidence="ECO:0000269|PubMed:17087772"
FT MUTAGEN 25
FT /note="K->A: Reduced DNA binding activity, hemolysin
FT production and virulence in silkworm-infection model."
FT /evidence="ECO:0000269|PubMed:17087772"
FT MUTAGEN 43
FT /note="V->M: Reduced DNA binding activity and hemolysin
FT production."
FT /evidence="ECO:0000269|PubMed:17087772"
FT MUTAGEN 67
FT /note="G->E: Reduced DNA binding activity, hemolysin
FT production and virulence in silkworm-infection model."
FT /evidence="ECO:0000269|PubMed:17087772"
FT MUTAGEN 70
FT /note="T->A: Reduced DNA binding activity, hemolysin
FT production and virulence in silkworm-infection model."
FT /evidence="ECO:0000269|PubMed:17087772"
FT MUTAGEN 82
FT /note="V->M: No effect."
FT /evidence="ECO:0000269|PubMed:17087772"
FT MUTAGEN 92
FT /note="R->C: Reduced DNA binding activity, hemolysin
FT production and virulence in silkworm-infection model."
FT /evidence="ECO:0000269|PubMed:17087772"
FT MUTAGEN 96
FT /note="I->A: Reduced DNA binding activity, hemolysin
FT production and virulence in silkworm-infection model."
FT /evidence="ECO:0000269|PubMed:17087772"
FT MUTAGEN 103
FT /note="K->A: No effect."
FT /evidence="ECO:0000269|PubMed:17087772"
FT MUTAGEN 124
FT /note="E->A: No effect."
FT /evidence="ECO:0000269|PubMed:17087772"
FT MUTAGEN 134
FT /note="L->A: No effect."
FT /evidence="ECO:0000269|PubMed:17087772"
FT MUTAGEN 140
FT /note="K->A: No effect."
FT /evidence="ECO:0000269|PubMed:17087772"
SQ SEQUENCE 148 AA; 17435 MW; 944CA3C0F058D870 CRC64;
MYVENSYLSK QLCFLFYVSS KEIIKKYTNY LKEYDLTYTG YIVLMAIEND EKLNIKKLGE
RVFLDSGTLT PLLKKLEKKD YVVRTREEKD ERNLQISLTE QGKAIKSPLA EISVKVFNEF
NISEREASDI INNLRNFVSK NFDYSDKK
