SAS10_DROME
ID SAS10_DROME Reviewed; 428 AA.
AC Q9I7W5; Q961T6;
DT 01-FEB-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2002, sequence version 2.
DT 03-AUG-2022, entry version 110.
DE RecName: Full=Something about silencing protein 10;
GN Name=Sas10 {ECO:0000312|EMBL:AAG22407.2}; ORFNames=CG4202;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1] {ECO:0000305, ECO:0000312|EMBL:AAG22407.2}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley {ECO:0000269|PubMed:10731132};
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2] {ECO:0000305, ECO:0000312|EMBL:AAG22407.2}
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3] {ECO:0000305, ECO:0000312|EMBL:AAK77307.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley {ECO:0000269|PubMed:12537569};
RC TISSUE=Head {ECO:0000269|PubMed:12537569};
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [4] {ECO:0000305}
RP FUNCTION, AND DEVELOPMENTAL STAGE.
RC STRAIN=Oregon-R {ECO:0000269|PubMed:12756328};
RX PubMed=12756328; DOI=10.1261/rna.2120703;
RA Peters N.T., Rohrbach J.A., Zalewski B.A., Byrkett C.M., Vaughn J.C.;
RT "RNA editing and regulation of Drosophila 4f-rnp expression by sas-10
RT antisense readthrough mRNA transcripts.";
RL RNA 9:698-710(2003).
RN [5]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-152; SER-323; SER-324 AND
RP SER-337, AND IDENTIFICATION BY MASS SPECTROMETRY.
RC TISSUE=Embryo;
RX PubMed=18327897; DOI=10.1021/pr700696a;
RA Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P.;
RT "Phosphoproteome analysis of Drosophila melanogaster embryos.";
RL J. Proteome Res. 7:1675-1682(2008).
CC -!- FUNCTION: Essential for gene silencing: has a role in the structure of
CC silenced chromatin. May be involved in gene regulation during
CC development. Binds RNA. {ECO:0000250|UniProtKB:Q12136,
CC ECO:0000269|PubMed:12756328, ECO:0000303|PubMed:12756328}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- DEVELOPMENTAL STAGE: The short transcript is expressed moderately
CC during early embryonic stages (0-18 hours) but then declines in late
CC stage embryos (15-21 hours), becoming replaced by the long transcript
CC which persists into the adult body. {ECO:0000269|PubMed:12756328}.
CC -!- MISCELLANEOUS: Transcription utilizes two promoters. Promoter 1 gives
CC rise to the short transcript encoding Sas10 only. Promoter 2 generates
CC a discistronic transcript of Sas10 and Rnp4F. The longer transcript
CC becomes an RNA duplex which is a target for RNA editing via Adar
CC editase; Rnp4F (not Sas10) is edited. {ECO:0000269|PubMed:12756328}.
CC -!- SIMILARITY: Belongs to the SAS10 family. {ECO:0000305}.
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DR EMBL; AE014298; AAG22407.2; -; Genomic_DNA.
DR EMBL; AY047575; AAK77307.1; -; mRNA.
DR RefSeq; NP_572215.1; NM_131987.4.
DR AlphaFoldDB; Q9I7W5; -.
DR SMR; Q9I7W5; -.
DR BioGRID; 57957; 1.
DR IntAct; Q9I7W5; 10.
DR STRING; 7227.FBpp0070719; -.
DR iPTMnet; Q9I7W5; -.
DR PaxDb; Q9I7W5; -.
DR PRIDE; Q9I7W5; -.
DR DNASU; 31447; -.
DR EnsemblMetazoa; FBtr0070751; FBpp0070719; FBgn0029755.
DR GeneID; 31447; -.
DR KEGG; dme:Dmel_CG4202; -.
DR UCSC; CG4202-RA; d. melanogaster.
DR CTD; 31447; -.
DR FlyBase; FBgn0029755; Sas10.
DR VEuPathDB; VectorBase:FBgn0029755; -.
DR eggNOG; KOG3118; Eukaryota.
DR GeneTree; ENSGT00500000044947; -.
DR HOGENOM; CLU_025161_1_0_1; -.
DR InParanoid; Q9I7W5; -.
DR OMA; EEYIRPQ; -.
DR OrthoDB; 1451774at2759; -.
DR PhylomeDB; Q9I7W5; -.
DR Reactome; R-DME-6791226; Major pathway of rRNA processing in the nucleolus and cytosol.
DR SignaLink; Q9I7W5; -.
DR BioGRID-ORCS; 31447; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 31447; -.
DR PRO; PR:Q9I7W5; -.
DR Proteomes; UP000000803; Chromosome X.
DR Bgee; FBgn0029755; Expressed in eye disc (Drosophila) and 39 other tissues.
DR Genevisible; Q9I7W5; DM.
DR GO; GO:0005730; C:nucleolus; IBA:GO_Central.
DR GO; GO:0032040; C:small-subunit processome; IBA:GO_Central.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR GO; GO:0006325; P:chromatin organization; IEA:UniProtKB-KW.
DR GO; GO:0000462; P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA); IBA:GO_Central.
DR InterPro; IPR007146; Sas10/Utp3/C1D.
DR InterPro; IPR018972; Sas10_C_dom.
DR PANTHER; PTHR13237; PTHR13237; 2.
DR Pfam; PF09368; Sas10; 1.
DR Pfam; PF04000; Sas10_Utp3; 1.
PE 1: Evidence at protein level;
KW Chromatin regulator; Developmental protein; Nucleus; Phosphoprotein;
KW Reference proteome; RNA-binding.
FT CHAIN 1..428
FT /note="Something about silencing protein 10"
FT /id="PRO_0000114329"
FT REGION 1..93
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 317..386
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..22
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 23..43
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 44..64
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 323..352
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 152
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MOD_RES 323
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MOD_RES 324
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MOD_RES 337
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
SQ SEQUENCE 428 AA; 49454 MW; 13EE01284985A091 CRC64;
MDSDGDDYVM SGSDQEYDDE EREILEDLRK QRKKPHDPVQ EVLGFSDDDD DDDDDDEEEQ
QDVAELMRDS DIEGAEDDDR DLPNTMDWGS KRSTYYNTDF VDQDYSSYNA QEEEQARAEE
EEAKKIQLRL AKRMSEADFQ LDNVEAAPAG SSDDNELSHI TKVTSDLAGL SERERRQLMH
SESPEFIILT QDFQQHLDEV KNLLKPVLNY VRKHDVPMVP ALQYAGLCHT VLTTYCSNVA
FYLLLKARRI DVKAHPVIRR LVQLKDLIEE LKPRYEEYIR PQLEALLERI EDGDAFTVLD
VAQRKAKLQI LNKYNDGQQA SVSSDDDDND DDDDAESKEK DLQEEAGEEE EEEDARRGIT
YQMAKNKGLT PHRKKELRNP RVKHRGKYRK ALIRRKGAVR TVRKELQRYG GELSGIKAGV
TKSVKFRT
