SAS4_CAEEL
ID SAS4_CAEEL Reviewed; 808 AA.
AC P34402;
DT 01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT 15-MAY-2002, sequence version 2.
DT 03-AUG-2022, entry version 124.
DE RecName: Full=Spindle assembly abnormal protein 4;
GN Name=sas-4; ORFNames=F10E9.8;
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, AND
RP DEVELOPMENTAL STAGE.
RX PubMed=12636923; DOI=10.1016/s1534-5807(03)00062-5;
RA Leidel S., Goenczy P.;
RT "SAS-4 is essential for centrosome duplication in C. elegans and is
RT recruited to daughter centrioles once per cell cycle.";
RL Dev. Cell 4:431-439(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2;
RX PubMed=7906398; DOI=10.1038/368032a0;
RA Wilson R., Ainscough R., Anderson K., Baynes C., Berks M., Bonfield J.,
RA Burton J., Connell M., Copsey T., Cooper J., Coulson A., Craxton M.,
RA Dear S., Du Z., Durbin R., Favello A., Fraser A., Fulton L., Gardner A.,
RA Green P., Hawkins T., Hillier L., Jier M., Johnston L., Jones M.,
RA Kershaw J., Kirsten J., Laisster N., Latreille P., Lightning J., Lloyd C.,
RA Mortimore B., O'Callaghan M., Parsons J., Percy C., Rifken L., Roopra A.,
RA Saunders D., Shownkeen R., Sims M., Smaldon N., Smith A., Smith M.,
RA Sonnhammer E., Staden R., Sulston J., Thierry-Mieg J., Thomas K.,
RA Vaudin M., Vaughan K., Waterston R., Watson A., Weinstock L.,
RA Wilkinson-Sproat J., Wohldman P.;
RT "2.2 Mb of contiguous nucleotide sequence from chromosome III of C.
RT elegans.";
RL Nature 368:32-38(1994).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2;
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [4]
RP FUNCTION, SUBCELLULAR LOCATION, AND DEVELOPMENTAL STAGE.
RX PubMed=12600319; DOI=10.1016/s0092-8674(03)00117-x;
RA Kirkham M., Mueller-Reichert T., Oegema K., Grill S., Hyman A.A.;
RT "SAS-4 is a C. elegans centriolar protein that controls centrosome size.";
RL Cell 112:575-587(2003).
RN [5]
RP INTERACTION WITH HYLS-1.
RX PubMed=19656802; DOI=10.1101/gad.1810409;
RA Dammermann A., Pemble H., Mitchell B.J., McLeod I., Yates J.R. III,
RA Kintner C., Desai A.B., Oegema K.;
RT "The hydrolethalus syndrome protein HYLS-1 links core centriole structure
RT to cilia formation.";
RL Genes Dev. 23:2046-2059(2009).
CC -!- FUNCTION: Required for centrosome duplication. Plays a central role in
CC determining centrosome size. {ECO:0000269|PubMed:12600319,
CC ECO:0000269|PubMed:12636923}.
CC -!- SUBUNIT: Interacts with hyls-1; leading to hyls-1 localization into
CC newly forming centrioles. {ECO:0000269|PubMed:19656802}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, microtubule organizing
CC center, centrosome {ECO:0000269|PubMed:12600319,
CC ECO:0000269|PubMed:12636923}. Note=Localizes to the centrosome
CC throughout the cell cycle. Localizes to a tiny dot in the center of
CC centrosome. Recruited to the centrosome once per cell cycle, at the
CC time of organelle duplication and remains stably associated after.
CC -!- DEVELOPMENTAL STAGE: Localizes to centrosomes both in sperm and in the
CC syncytial part of the gonad. Staining in the gonad disappears as the
CC meiotic nuclei cellularizes to form oocytes, presumably marking the
CC point at which the centrioles are lost during oogenesis. In newly
CC fertilized embryos, it is localized to a discrete spot near the sperm-
CC derived pronucleus. Then, it remains attached to the centrosome
CC throughout the rest of development. {ECO:0000269|PubMed:12600319,
CC ECO:0000269|PubMed:12636923}.
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DR EMBL; AJ539470; CAD62434.1; -; mRNA.
DR EMBL; FO081105; CCD69136.1; -; Genomic_DNA.
DR RefSeq; NP_498828.2; NM_066427.5.
DR AlphaFoldDB; P34402; -.
DR SMR; P34402; -.
DR BioGRID; 41376; 21.
DR IntAct; P34402; 12.
DR STRING; 6239.F10E9.8; -.
DR EPD; P34402; -.
DR PaxDb; P34402; -.
DR PRIDE; P34402; -.
DR EnsemblMetazoa; F10E9.8.1; F10E9.8.1; WBGene00004726.
DR GeneID; 176172; -.
DR KEGG; cel:CELE_F10E9.8; -.
DR UCSC; F10E9.8; c. elegans.
DR CTD; 40859; -.
DR WormBase; F10E9.8; CE29756; WBGene00004726; sas-4.
DR eggNOG; ENOG502SYI4; Eukaryota.
DR HOGENOM; CLU_018365_0_0_1; -.
DR InParanoid; P34402; -.
DR OMA; EFRLRWY; -.
DR OrthoDB; 1111973at2759; -.
DR PRO; PR:P34402; -.
DR Proteomes; UP000001940; Chromosome III.
DR Bgee; WBGene00004726; Expressed in germ line (C elegans) and 4 other tissues.
DR GO; GO:0005814; C:centriole; IDA:UniProtKB.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
DR GO; GO:0007099; P:centriole replication; IMP:WormBase.
DR GO; GO:0045185; P:maintenance of protein location; IMP:WormBase.
PE 1: Evidence at protein level;
KW Cell cycle; Coiled coil; Cytoplasm; Cytoskeleton; Developmental protein;
KW Reference proteome.
FT CHAIN 1..808
FT /note="Spindle assembly abnormal protein 4"
FT /id="PRO_0000097592"
FT REGION 1..151
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 187..206
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 271..298
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 511..564
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 161..181
FT /evidence="ECO:0000255"
FT COILED 314..503
FT /evidence="ECO:0000255"
FT COMPBIAS 30..107
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 276..296
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 514..557
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 808 AA; 92206 MW; 722C4509D3ACA97B CRC64;
MASDENIGAD GEQKPSRPFL RKGQGTARFR MPRNNKTSAG APPTSELSSA SSPSINVPRF
SLSNALPNSA RTVDSGISNE DETRPPTTAS LPMDQPSLSS SPENRLNPAP SVAEEHGHSG
QHAEEEEDND TDEVSAMPSF VPDEPSTLVN SDHELSDDAL KYKNAAAEFK AFERRMDSMR
SASTITTSLA TPSSCAPSNS SEPPTRSTPI MNDLGVGPNN HNWPSSMQEL SGISLETPQA
RPLGSNRINQ LVRSEAQTGI SLLQHHERPT VTAPLRRNDM MNSSRQNPQN GNVQDENRPE
HVYDQPIHVP GSSLDRQKLE IEIRRHRNLN IQLRDTIAHL DYAEESVHTT KRQLEEKISE
VNNFKKELIE EFKKCKKGVE EEFEKKFEKI KEDYDELYEK LKRDQRDLER DQKILKKGTG
ERNKEFTETI ATLRDKLRAS ETKNAQYRQD IRVRDEKLKK KDEEIEKLQK DGNRLKSTLQ
TLEKRVKQLR TEKERDDKEK EMFAKVAMNR KTSNPVPPVL NQSVPISITS NGPSRHPSSS
SLTTFRKPST SNRERGVSWA DEPNEQSLEA VPQEFLMMPV KEMPGKFGKC TIYRDSLGET
SKVTDTIANG LLFEYSNGDL RWVNRQNAVN IYISAVDKTV RIDLPTYNIS IIHTFQRQVE
VLRPGNNITL ISIKRREVRT DLIYQNGMYK TEIFNRDGRY VTKDFSNQEV SRKYNPGTHT
YRDNQCRYVL VTDYNDFELV EPEFRLRWYQ GDPTGLNNQY ILKIIGRPEC SEKTLRLEVN
LSTCEGTLET AEMIGDKRRK TTLFQWKK
