ID   SASF_STAA8              Reviewed;         635 AA.
AC   Q2FUW9;
DT   10-OCT-2018, integrated into UniProtKB/Swiss-Prot.
DT   21-MAR-2006, sequence version 1.
DT   25-MAY-2022, entry version 66.
DE   RecName: Full=Surface protein F {ECO:0000303|PubMed:18800056};
DE   Flags: Precursor;
GN   Name=sasF {ECO:0000303|PubMed:18800056}; OrderedLocusNames=SAOUHSC_02982;
OS   Staphylococcus aureus (strain NCTC 8325 / PS 47).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC   Staphylococcus.
OX   NCBI_TaxID=93061;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=NCTC 8325 / PS 47;
RA   Gillaspy A.F., Worrell V., Orvis J., Roe B.A., Dyer D.W., Iandolo J.J.;
RT   "The Staphylococcus aureus NCTC 8325 genome.";
RL   (In) Fischetti V., Novick R., Ferretti J., Portnoy D., Rood J. (eds.);
RL   Gram positive pathogens, 2nd edition, pp.381-412, ASM Press, Washington
RL   D.C. (2006).
RN   [2]
RP   SUBCELLULAR LOCATION, AND PROCESSING BY SORTASE A.
RC   STRAIN=RN4220;
RX   PubMed=11830639; DOI=10.1073/pnas.032523999;
RA   Mazmanian S.K., Ton-That H., Su K., Schneewind O.;
RT   "An iron-regulated sortase anchors a class of surface protein during
RT   Staphylococcus aureus pathogenesis.";
RL   Proc. Natl. Acad. Sci. U.S.A. 99:2293-2298(2002).
RN   [3]
RP   SUBCELLULAR LOCATION.
RC   STRAIN=RN4220;
RX   PubMed=18800056; DOI=10.1038/emboj.2008.185;
RA   DeDent A., Bae T., Missiakas D.M., Schneewind O.;
RT   "Signal peptides direct surface proteins to two distinct envelope locations
RT   of Staphylococcus aureus.";
RL   EMBO J. 27:2656-2668(2008).
CC   -!- SUBCELLULAR LOCATION: Secreted, cell wall {ECO:0000269|PubMed:18800056,
CC       ECO:0000305|PubMed:11830639}; Peptidoglycan-anchor
CC       {ECO:0000269|PubMed:18800056, ECO:0000305|PubMed:11830639}.
CC       Note=Distributed in a discrete, punctate pattern with up to 3 loci per
CC       cell over the surface (PubMed:18800056). Does not localize with ClfA
CC       (PubMed:18800056). Exchanging the ClfA and SasF signal peptides
CC       retargets the mature protein on the cell surface (PubMed:18800056).
CC       Anchored to the cell wall by sortase A (Probable).
CC       {ECO:0000269|PubMed:18800056, ECO:0000305|PubMed:11830639}.
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DR   EMBL; CP000253; ABD31969.1; -; Genomic_DNA.
DR   RefSeq; WP_001151900.1; NZ_LS483365.1.
DR   RefSeq; YP_501431.1; NC_007795.1.
DR   AlphaFoldDB; Q2FUW9; -.
DR   SMR; Q2FUW9; -.
DR   STRING; 1280.SAXN108_2917; -.
DR   EnsemblBacteria; ABD31969; ABD31969; SAOUHSC_02982.
DR   GeneID; 3921464; -.
DR   KEGG; sao:SAOUHSC_02982; -.
DR   PATRIC; fig|93061.5.peg.2689; -.
DR   eggNOG; ENOG503051Q; Bacteria.
DR   HOGENOM; CLU_029259_0_0_9; -.
DR   OMA; NKGSQIE; -.
DR   Proteomes; UP000008816; Chromosome.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-KW.
DR   InterPro; IPR002988; GA_module.
DR   Pfam; PF01468; GA; 1.
PE   3: Inferred from homology;
KW   Cell wall; Peptidoglycan-anchor; Reference proteome; Secreted; Signal.
FT   SIGNAL          1..37
FT                   /evidence="ECO:0000255"
FT   CHAIN           38..635
FT                   /note="Surface protein F"
FT                   /id="PRO_5004208097"
FT   PROPEP          601..635
FT                   /note="Removed by sortase"
FT                   /evidence="ECO:0000305|PubMed:11830639,
FT                   ECO:0000305|PubMed:18800056"
FT                   /id="PRO_0000445583"
FT   REGION          101..122
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          192..228
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOTIF           597..601
FT                   /note="LPXTG sorting signal"
FT                   /evidence="ECO:0000305|PubMed:18800056"
FT   COMPBIAS        203..228
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOD_RES         600
FT                   /note="Pentaglycyl murein peptidoglycan amidated alanine"
FT                   /evidence="ECO:0000305|PubMed:18800056"
SQ   SEQUENCE   635 AA;  70932 MW;  F97780D235A02F95 CRC64;
     MAKYRGKPFQ LYVKLSCSTM MATSIILTNI LPYDAQAASE KDTEITKEIL SKQDLLDKVD
     KAIRQIEQLK QLSASSKEHY KAQLNEAKTA SQIDEIIKRA NELDSKDNKS SHTEMNGQSD
     IDSKLDQLLK DLNEVSSNVD RGQQSGEDDL NAMKNDMSQT ATTKHGEKDD KNDEAMVNKA
     LEDLDHLNQQ IHKSKDASKD TSEDPAVSTT DNNHEVAKTP NNDGSGHVVL NKFLSNEENQ
     SHSNRLTDKL QGSDKINHAM IEKLAKSNAS TQHYTYHKLN TLQSLDQRIA NTQLPKNQKS
     DLMSEVNKTK ERIKSQRNII LEELARTDDK KYATQSILES IFNKDEAVKI LKDIRVDGKT
     DQQIADQITR HIDQLSLTTS DDLLTSLIDQ SQDKSLLISQ ILQTKLGKAE ADKLAKDWTN
     KGLSNRQIVD QLKKHFASTG DTSSDDILKA ILNNAKDKKQ AIETILATRI ERQKAKLLAD
     LITKIETDQN KIFNLVKSAL NGKADDLLNL QKRLNQTKKD IDYILSPIVN RPSLLDRLNK
     NGKTTDLNKL ANLMNQGSDL LDSIPDIPTP KPEKTLTLGK GNGLLSGLLN ADGNVSLPKA
     GETIKEHWLP ISVIVGAMGV LMIWLSRRNK LKNKA