ID   SASG_PRIMG              Reviewed;          97 AA.
AC   P02961;
DT   21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT   23-JAN-2007, sequence version 2.
DT   25-MAY-2022, entry version 64.
DE   RecName: Full=Small, acid-soluble spore protein gamma-type;
DE            Short=SASP;
GN   Name=sasP-B;
OS   Priestia megaterium (Bacillus megaterium).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Priestia.
OX   NCBI_TaxID=1404;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=2430935; DOI=10.1128/jb.168.2.1023-1025.1986;
RA   Hackett R.H., Setlow B., Setlow P.;
RT   "Cloning and nucleotide sequence of the Bacillus megaterium gene coding for
RT   small, acid-soluble spore protein B.";
RL   J. Bacteriol. 168:1023-1025(1986).
RN   [2]
RP   PROTEIN SEQUENCE OF 2-44 AND 68-79.
RX   PubMed=6767722; DOI=10.1016/s0021-9258(19)85749-6;
RA   Setlow P., Gerard C., Ozols J.;
RT   "The amino acid sequence specificity of a protease from spores of Bacillus
RT   megaterium.";
RL   J. Biol. Chem. 255:3624-3628(1980).
RN   [3]
RP   PROTEIN SEQUENCE OF 33-97.
RX   PubMed=6776116; DOI=10.1016/s0021-9258(19)70484-0;
RA   Setlow P., Ozols J.;
RT   "The complete covalent structure of protein B. The third major protein
RT   degraded during germination of Bacillus megaterium spores.";
RL   J. Biol. Chem. 255:10445-10450(1980).
CC   -!- FUNCTION: SASP are proteins degraded in the first minutes of spore
CC       germination and provide amino acids for both new protein synthesis and
CC       metabolism. These proteins may be involved in dormant spore's high
CC       resistance to UV light.
CC   -!- SIMILARITY: Belongs to the gamma-type SASP family. {ECO:0000305}.
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DR   EMBL; M14161; AAA22293.1; -; Genomic_DNA.
DR   PIR; A25162; USBSBM.
DR   RefSeq; WP_013055161.1; NZ_VXLA01000100.1.
DR   AlphaFoldDB; P02961; -.
DR   PRIDE; P02961; -.
DR   GeneID; 64148892; -.
DR   GO; GO:0030435; P:sporulation resulting in formation of a cellular spore; IEA:UniProtKB-KW.
DR   InterPro; IPR006341; Spore_gamma.
DR   Pfam; PF04259; SASP_gamma; 1.
DR   TIGRFAMs; TIGR01442; SASP_gamma; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Repeat; Sporulation.
FT   INIT_MET        1
FT                   /note="Removed"
FT                   /evidence="ECO:0000269|PubMed:6767722"
FT   CHAIN           2..97
FT                   /note="Small, acid-soluble spore protein gamma-type"
FT                   /id="PRO_0000196323"
FT   REPEAT          23..56
FT   REPEAT          58..91
FT   REGION          1..97
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   SITE            32..33
FT                   /note="Cleavage; by spore protease"
FT                   /evidence="ECO:0000269|PubMed:6776116"
FT   SITE            67..68
FT                   /note="Cleavage; by spore protease"
FT                   /evidence="ECO:0000269|PubMed:6776116"
FT   CONFLICT        21
FT                   /note="N -> D (in Ref. 2; AA sequence)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   97 AA;  10438 MW;  D7C271B3D849F5F0 CRC64;
     MAKQTNKTAS GTSTQHVKQQ NAQASKNNFG TEFGSETNVQ EVKQQNAQAA ANKSQNAQAS
     KNNFGTEFAS ETSAQEVRQQ NAQAQAKKNQ NSGKYQG