ID   SAUBS_SANAS             Reviewed;         576 AA.
AC   E3W205; F6M8H9;
DT   05-SEP-2012, integrated into UniProtKB/Swiss-Prot.
DT   03-OCT-2012, sequence version 2.
DT   03-AUG-2022, entry version 32.
DE   RecName: Full=Beta-bisabolene synthase;
DE            Short=SauBS;
DE            EC=4.2.3.-;
OS   Santalum austrocaledonicum (Sandalwood).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   Santalales; Santalaceae; Santalum.
OX   NCBI_TaxID=293154;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], AND FUNCTION.
RX   PubMed=21454632; DOI=10.1074/jbc.m111.231787;
RA   Jones C.G., Moniodis J., Zulak K.G., Scaffidi A., Plummer J.A.,
RA   Ghisalberti E.L., Barbour E.L., Bohlmann J.;
RT   "Sandalwood fragrance biosynthesis involves sesquiterpene synthases of both
RT   the terpene synthase (TPS)-a and TPS-b Subfamilies, including santalene
RT   synthases.";
RL   J. Biol. Chem. 286:17445-17454(2011).
CC   -!- FUNCTION: Produces almost exclusively beta-bisabolene and only traces
CC       of alpha-bisabolol from (2E,6E)-farnesyl diphosphate in fragrance
CC       biosynthesis. {ECO:0000269|PubMed:21454632}.
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250};
CC       Name=Mn(2+); Xref=ChEBI:CHEBI:29035; Evidence={ECO:0000250};
CC       Note=Binds 3 Mg(2+) or Mn(2+) ions per subunit. {ECO:0000250};
CC   -!- SIMILARITY: Belongs to the terpene synthase family. Tpsb subfamily.
CC       {ECO:0000305}.
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DR   EMBL; HQ343279; ADO87003.1; -; mRNA.
DR   EMBL; JF746813; AEF32531.1; -; Genomic_DNA.
DR   AlphaFoldDB; E3W205; -.
DR   SMR; E3W205; -.
DR   GO; GO:0000287; F:magnesium ion binding; IEA:InterPro.
DR   GO; GO:0010333; F:terpene synthase activity; IEA:InterPro.
DR   GO; GO:0016102; P:diterpenoid biosynthetic process; IEA:InterPro.
DR   CDD; cd00684; Terpene_cyclase_plant_C1; 1.
DR   Gene3D; 1.10.600.10; -; 1.
DR   Gene3D; 1.50.10.130; -; 1.
DR   InterPro; IPR008949; Isoprenoid_synthase_dom_sf.
DR   InterPro; IPR034741; Terpene_cyclase-like_1_C.
DR   InterPro; IPR044814; Terpene_cyclase_plant_C1.
DR   InterPro; IPR001906; Terpene_synth_N.
DR   InterPro; IPR036965; Terpene_synth_N_sf.
DR   InterPro; IPR005630; Terpene_synthase_metal-bd.
DR   InterPro; IPR008930; Terpenoid_cyclase/PrenylTrfase.
DR   Pfam; PF01397; Terpene_synth; 1.
DR   Pfam; PF03936; Terpene_synth_C; 1.
DR   SFLD; SFLDG01019; Terpene_Cyclase_Like_1_C_Termi; 1.
DR   SUPFAM; SSF48239; SSF48239; 1.
DR   SUPFAM; SSF48576; SSF48576; 1.
PE   2: Evidence at transcript level;
KW   Lyase; Magnesium; Manganese; Metal-binding.
FT   CHAIN           1..576
FT                   /note="Beta-bisabolene synthase"
FT                   /id="PRO_0000418945"
FT   MOTIF           323..327
FT                   /note="DDXXD motif"
FT   BINDING         323
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000250"
FT   BINDING         323
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000250"
FT   BINDING         327
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000250"
FT   BINDING         327
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000250"
FT   BINDING         473
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /ligand_label="3"
FT                   /evidence="ECO:0000250"
FT   BINDING         477
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /ligand_label="3"
FT                   /evidence="ECO:0000250"
FT   CONFLICT        202
FT                   /note="K -> E (in Ref. 1; ADO87003)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   576 AA;  65781 MW;  6F9BB53DFF36C8B1 CRC64;
     MDAFATSPTS ALIKAVNCIA HVTPMAGEDS SENRRASNYK PSTWDYEFLQ SLATSHNTVQ
     EKHMKMAEKL KEEVKSMIKG QMEPVAKLEL INIVQRLGLK YRFESEIKEE LFSLYKDGTD
     AWWVDNLHAT ALRFRLLREN GIFVPQDVFE TFKDKSGKFK SQLCKDVRGL LSLYEASYLG
     WEGEDLLDEA KKFSTTNLNN VKESISSNTL GRLVKHALNL PLHWSAARYE ARWFIDEYEK
     EENVNPNLLK YAKLDFNIVQ SIHQGELGNL ARWWVETGLD KLSFVRNTLM QNFMWGCAMV
     FEPQYGKVRD AAVKQASLIA MVDDVYDVYG SLEELEIFTD IVDRWDITGI DKLPRNISMI
     LLTMFNTANQ IGYDLLRDRG FNGIPHIAQA WATLCKKYLK EAKWYHSGYK PTLEEYLENG
     LVSISFVLSL VTAYLQTETL ENLTYESAAY VNSVPPLVRY SGLLNRLYND LGTSSAEIAR
     GDTLKSIQCY MTQTGATEEA AREHIKGLVH EAWKGMNKCL FEQTPFAEPF VGFNVNTVRG
     SQFFYQHGDG YAVTESWTKD LSLSVLIHPI PLNEED