ID   SAUSS_SANAS             Reviewed;         559 AA.
AC   E3W207;
DT   05-SEP-2012, integrated into UniProtKB/Swiss-Prot.
DT   11-JAN-2011, sequence version 1.
DT   03-AUG-2022, entry version 31.
DE   RecName: Full=Sesquiterpene synthase;
DE            Short=SauSesquiTPS;
DE            EC=4.2.3.-;
OS   Santalum austrocaledonicum (Sandalwood).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   Santalales; Santalaceae; Santalum.
OX   NCBI_TaxID=293154;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], AND FUNCTION.
RX   PubMed=21454632; DOI=10.1074/jbc.m111.231787;
RA   Jones C.G., Moniodis J., Zulak K.G., Scaffidi A., Plummer J.A.,
RA   Ghisalberti E.L., Barbour E.L., Bohlmann J.;
RT   "Sandalwood fragrance biosynthesis involves sesquiterpene synthases of both
RT   the terpene synthase (TPS)-a and TPS-b Subfamilies, including santalene
RT   synthases.";
RL   J. Biol. Chem. 286:17445-17454(2011).
CC   -!- FUNCTION: Catalyzes alpha-humulene and delta-cadinene, as well as beta-
CC       elemene, the thermal rearrangement product of germacrene A and several
CC       other bicyclic sesquiterpenes when incubated with (2E,6E)-farnesyl
CC       diphosphate. {ECO:0000269|PubMed:21454632}.
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250};
CC       Name=Mn(2+); Xref=ChEBI:CHEBI:29035; Evidence={ECO:0000250};
CC       Note=Binds 3 Mg(2+) or Mn(2+) ions per subunit. {ECO:0000250};
CC   -!- SIMILARITY: Belongs to the terpene synthase family. Tpsa subfamily.
CC       {ECO:0000305}.
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DR   EMBL; HQ343281; ADO87005.1; -; mRNA.
DR   AlphaFoldDB; E3W207; -.
DR   SMR; E3W207; -.
DR   PRIDE; E3W207; -.
DR   GO; GO:0000287; F:magnesium ion binding; IEA:InterPro.
DR   GO; GO:0010333; F:terpene synthase activity; IEA:InterPro.
DR   GO; GO:0016102; P:diterpenoid biosynthetic process; IEA:InterPro.
DR   CDD; cd00684; Terpene_cyclase_plant_C1; 1.
DR   Gene3D; 1.10.600.10; -; 1.
DR   Gene3D; 1.50.10.130; -; 1.
DR   InterPro; IPR008949; Isoprenoid_synthase_dom_sf.
DR   InterPro; IPR044814; Terpene_cyclase_plant_C1.
DR   InterPro; IPR001906; Terpene_synth_N.
DR   InterPro; IPR036965; Terpene_synth_N_sf.
DR   InterPro; IPR005630; Terpene_synthase_metal-bd.
DR   InterPro; IPR008930; Terpenoid_cyclase/PrenylTrfase.
DR   Pfam; PF01397; Terpene_synth; 1.
DR   Pfam; PF03936; Terpene_synth_C; 1.
DR   SUPFAM; SSF48239; SSF48239; 1.
DR   SUPFAM; SSF48576; SSF48576; 1.
PE   2: Evidence at transcript level;
KW   Lyase; Magnesium; Manganese; Metal-binding.
FT   CHAIN           1..559
FT                   /note="Sesquiterpene synthase"
FT                   /id="PRO_0000418947"
FT   MOTIF           312..316
FT                   /note="DDXXD motif"
FT   BINDING         312
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000250"
FT   BINDING         312
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000250"
FT   BINDING         316
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000250"
FT   BINDING         316
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000250"
FT   BINDING         464
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /ligand_label="3"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   559 AA;  65559 MW;  F05080986CA11303 CRC64;
     MENQKVPISS VPNLKELSRP IANFPPSIWG DRFINYTCED ENEQAQKERQ VEELKEQVRR
     ELAATVDKPL QQLNIIDATQ RLGIAYHFEN EIEESLEHIY LHTYVKNNCF QGSHDLYSVA
     LWFRLLRQDG YKVSCDVFDN FRDYEGNFKN SLMEDAKGLL ELYEATHLSV HGEEMLDDAL
     EFAKTRLESI VNHLNYPLAE QVRHALYRPL RKGLPRLEAV YFFRIYEAYH SHNKALLKLA
     KLDFNLLQSL HRKELGDMAR WWRSLDFATK FPFARDRLVE GYFWILGVYF EPQYSLAREI
     TTKVFAMIST IDDIYDAYGT LDELKLFTEA IQRWDVGSLD QLPEYMKPCY KSILDVYNEI
     EEEMANQGSL FRMHYAKEVM KTIVEGYMDE AKWCHEKYVP TFQEYMSLAL VTSGYTFLTT
     ISYLGMGGIA SKEAFEWLFS HPPIIEASES ICRLMDDMSS HKFEQERGHV ASGIECYMKQ
     YGVIEEEAHD EFHKRLVKAW KDINEGFLRP YAVPEPLLMR ILNLTRVMDV IYKNEDSYTH
     VKKAMKDNIA SLLIDPMIV