ID   SAUST_SANAS             Reviewed;         562 AA.
AC   F6M8H5;
DT   03-OCT-2012, integrated into UniProtKB/Swiss-Prot.
DT   27-JUL-2011, sequence version 1.
DT   03-AUG-2022, entry version 31.
DE   RecName: Full=Probable sesquiterpene synthase;
DE            Short=SauSTPS;
DE            EC=4.2.3.-;
GN   Name=SesquiTPS;
OS   Santalum austrocaledonicum (Sandalwood).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   Santalales; Santalaceae; Santalum.
OX   NCBI_TaxID=293154;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=21454632; DOI=10.1074/jbc.m111.231787;
RA   Jones C.G., Moniodis J., Zulak K.G., Scaffidi A., Plummer J.A.,
RA   Ghisalberti E.L., Barbour E.L., Bohlmann J.;
RT   "Sandalwood fragrance biosynthesis involves sesquiterpene synthases of both
RT   the terpene synthase (TPS)-a and TPS-b Subfamilies, including santalene
RT   synthases.";
RL   J. Biol. Chem. 286:17445-17454(2011).
CC   -!- FUNCTION: Sesquiterpene synthase. {ECO:0000250}.
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250};
CC       Name=Mn(2+); Xref=ChEBI:CHEBI:29035; Evidence={ECO:0000250};
CC       Note=Binds 3 Mg(2+) or Mn(2+) ions per subunit. {ECO:0000250};
CC   -!- SIMILARITY: Belongs to the terpene synthase family. Tpsa subfamily.
CC       {ECO:0000305}.
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DR   EMBL; JF746808; AEF32535.1; -; Genomic_DNA.
DR   AlphaFoldDB; F6M8H5; -.
DR   SMR; F6M8H5; -.
DR   PRIDE; F6M8H5; -.
DR   GO; GO:0000287; F:magnesium ion binding; IEA:InterPro.
DR   GO; GO:0010333; F:terpene synthase activity; IEA:InterPro.
DR   GO; GO:0016102; P:diterpenoid biosynthetic process; IEA:InterPro.
DR   CDD; cd00684; Terpene_cyclase_plant_C1; 1.
DR   Gene3D; 1.10.600.10; -; 1.
DR   Gene3D; 1.50.10.130; -; 1.
DR   InterPro; IPR008949; Isoprenoid_synthase_dom_sf.
DR   InterPro; IPR034741; Terpene_cyclase-like_1_C.
DR   InterPro; IPR044814; Terpene_cyclase_plant_C1.
DR   InterPro; IPR001906; Terpene_synth_N.
DR   InterPro; IPR036965; Terpene_synth_N_sf.
DR   InterPro; IPR005630; Terpene_synthase_metal-bd.
DR   InterPro; IPR008930; Terpenoid_cyclase/PrenylTrfase.
DR   Pfam; PF01397; Terpene_synth; 1.
DR   Pfam; PF03936; Terpene_synth_C; 1.
DR   SFLD; SFLDG01019; Terpene_Cyclase_Like_1_C_Termi; 1.
DR   SUPFAM; SSF48239; SSF48239; 1.
DR   SUPFAM; SSF48576; SSF48576; 1.
PE   3: Inferred from homology;
KW   Lyase; Magnesium; Manganese; Metal-binding.
FT   CHAIN           1..562
FT                   /note="Probable sesquiterpene synthase"
FT                   /id="PRO_0000419326"
FT   MOTIF           315..319
FT                   /note="DDXXD motif"
FT   BINDING         315
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000250"
FT   BINDING         315
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000250"
FT   BINDING         319
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000250"
FT   BINDING         319
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000250"
FT   BINDING         467
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /ligand_label="3"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   562 AA;  65922 MW;  1038B707473AAC50 CRC64;
     MENQKMPISS VSNLKDLNMI SRPVANFPPS IWGDRFINYA CEDENDQAQK EKQVEELKEL
     VRRELAITVD KPLQQLNIID ATQRLGIAYL FENEIEESLE HIYLHTYVEN TCFEGSDDLY
     SVALWFRLLR QNDYRVSCDV FNKFRDNEGN FKNNLMEDTK GLLELYEATH LSVHGEEMLD
     DALEFTKTRL ESVVSHLNYP LAEQVRHALY QPLHKGLPRL EAVYFFRIYE AHASHNKALL
     KLAKLDFNLL QSFHKKELSD IARWWKSLDF AAKFPFARDR LVEGYFWVLG VYFEPQYSLA
     RKIIIKVFTM ISTIDDIYDA YGTLDELELF TKAMQRWDVG SLDQLPEYMK PCYKSILDVY
     NEIEVEMDNQ GSLFRMHYAK EVMKKLVEGY MDEAKWCHEK YVPTFQEYMS VALVTSGYTF
     LTTISYLGMG EIASKEAFDW LFSHPPVIEA SESVGRLMDD TRSHKFEQER GHVASGIECY
     MKQYGVTEEE ARDEFRKRLV KAWKDINEEC LRPYRVPKPL LMRILNLTRV IDVIYKNEDG
     YTHVKKAMKD NIASLLIDPM IV