ID   SAUVE_PHYSA             Reviewed;          62 AA.
AC   C5J8E3;
DT   02-MAR-2010, integrated into UniProtKB/Swiss-Prot.
DT   28-JUL-2009, sequence version 1.
DT   25-MAY-2022, entry version 15.
DE   RecName: Full=Sauvatide {ECO:0000303|PubMed:19358833, ECO:0000312|EMBL:CAX48601.1};
DE   Flags: Precursor;
GN   Name=sauvatide {ECO:0000312|EMBL:CAX48601.1};
OS   Phyllomedusa sauvagei (Sauvage's leaf frog).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Neobatrachia; Hyloidea; Hylidae; Phyllomedusinae;
OC   Phyllomedusa.
OX   NCBI_TaxID=8395;
RN   [1] {ECO:0000312|EMBL:CAX48601.1}
RP   NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 49-58, FUNCTION,
RP   SUBCELLULAR LOCATION, TISSUE SPECIFICITY, MASS SPECTROMETRY, AND AMIDATION
RP   AT LYS-58.
RC   TISSUE=Skin {ECO:0000312|EMBL:CAX48601.1};
RX   PubMed=19358833; DOI=10.1016/j.bbrc.2009.04.003;
RA   Wang L., Zhou M., Zhou Z., Chen T., Walker B., Shaw C.;
RT   "Sauvatide - A novel amidated myotropic decapeptide from the skin secretion
RT   of the waxy monkey frog, Phyllomedusa sauvagei.";
RL   Biochem. Biophys. Res. Commun. 383:240-244(2009).
CC   -!- FUNCTION: Induces contraction of smooth muscle in isolated rat urinary
CC       bladder with an EC(50) value of 2.2nM. {ECO:0000269|PubMed:19358833}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:19358833}.
CC   -!- TISSUE SPECIFICITY: Expressed by the skin glands.
CC       {ECO:0000269|PubMed:19358833}.
CC   -!- MASS SPECTROMETRY: Mass=1166.04; Method=MALDI;
CC       Evidence={ECO:0000269|PubMed:19358833};
CC   -!- SIMILARITY: Belongs to the frog skin active peptide (FSAP) family.
CC       {ECO:0000255}.
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DR   EMBL; FM999986; CAX48601.1; -; mRNA.
DR   AlphaFoldDB; C5J8E3; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0006952; P:defense response; IEA:UniProtKB-KW.
DR   InterPro; IPR004275; Frog_antimicrobial_propeptide.
DR   Pfam; PF03032; FSAP_sig_propep; 1.
PE   1: Evidence at protein level;
KW   Amidation; Amphibian defense peptide; Cleavage on pair of basic residues;
KW   Direct protein sequencing; Secreted; Signal.
FT   SIGNAL          1..24
FT                   /evidence="ECO:0000255"
FT   PROPEP          25..46
FT                   /evidence="ECO:0000269|PubMed:19358833"
FT                   /id="PRO_0000391685"
FT   PEPTIDE         49..58
FT                   /note="Sauvatide"
FT                   /evidence="ECO:0000269|PubMed:19358833"
FT                   /id="PRO_5000471235"
FT   MOD_RES         58
FT                   /note="Lysine amide"
FT                   /evidence="ECO:0000269|PubMed:19358833"
SQ   SEQUENCE   62 AA;  7145 MW;  8E599AA948D3FCEC CRC64;
     MDILKKSLFL ILFLGLVSIS FCDGEKRQDD DEANESEEKK EIHEVEKRLR PAILVRTKGK
     GK