SAYP_DROME
ID SAYP_DROME Reviewed; 2006 AA.
AC Q9VWF2; C7LAD9; Q7JW70;
DT 26-APR-2005, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 3.
DT 03-AUG-2022, entry version 158.
DE RecName: Full=Supporter of activation of yellow protein;
DE AltName: Full=Protein enhancer of yellow 3;
GN Name=e(y)3; Synonyms=l(1)G0084, SAYP; ORFNames=CG12238;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Embryo;
RA Carlson J.W., Booth B., Frise E., Park S., Wan K.H., Yu C., Celniker S.E.;
RL Submitted (SEP-2009) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 25-2006.
RC STRAIN=Berkeley; TISSUE=Embryo;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [5]
RP FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE,
RP AND DOMAIN.
RX PubMed=15616585; DOI=10.1038/sj.emboj.7600508;
RA Shidlovskii Y.V., Krasnov A.N., Nikolenko J.V., Lebedeva L.A.,
RA Kopantseva M., Ermolaeva M.A., Ilyin Y.V., Nabirochkina E.N.,
RA Georgiev P.G., Georgieva S.G.;
RT "A novel multidomain transcription coactivator SAYP can also repress
RT transcription in heterochromatin.";
RL EMBO J. 24:97-107(2005).
CC -!- FUNCTION: Essential transcription regulator during early development.
CC Coactivates transcription of some euchromatin genes and repress
CC transcription in of euchromatin genes translocated to heterochromatin.
CC {ECO:0000269|PubMed:15616585}.
CC -!- INTERACTION:
CC Q9VWF2; A1Z6M0: Bap170; NbExp=5; IntAct=EBI-131186, EBI-126851;
CC Q9VWF2; P49846: Taf5; NbExp=5; IntAct=EBI-131186, EBI-15595394;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:15616585}. Cytoplasm
CC {ECO:0000269|PubMed:15616585}. Chromosome
CC {ECO:0000269|PubMed:15616585}. Note=On polytene chromosomes, it mainly
CC localizes to transcriptionally active euchromatin sites but also
CC localizes to heterochromatic regions such as the chromocenter and the
CC chromosome 4. Cytoplasmic in nursing cells and growing oocytes.
CC -!- TISSUE SPECIFICITY: Widely expressed. Highly expressed in ovary.
CC Expressed in nursing cells and growing oocytes at all stages of
CC development and accumulates in mature oocytes. Expressed in the nuclei
CC of syncytium blastoderm of early embryos and in the nuclei of different
CC tissues of late embryos, larvae, and adults.
CC {ECO:0000269|PubMed:15616585}.
CC -!- DEVELOPMENTAL STAGE: Expressed throughout all stages of development.
CC Expressed at higher level in adult females.
CC {ECO:0000269|PubMed:15616585}.
CC -!- DOMAIN: The PHD fingers mediate the transcription repression in
CC heterochromatin. {ECO:0000269|PubMed:15616585}.
CC -!- DOMAIN: The SAY domain is essential for fly viability and transcription
CC activation. {ECO:0000269|PubMed:15616585}.
CC -!- SIMILARITY: Belongs to the SAYP family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAM48352.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AE014298; AAF48990.3; -; Genomic_DNA.
DR EMBL; BT099689; ACV44475.1; -; mRNA.
DR EMBL; AY118323; AAM48352.1; ALT_INIT; mRNA.
DR RefSeq; NP_001259718.1; NM_001272789.1.
DR RefSeq; NP_001259721.1; NM_001272792.1.
DR RefSeq; NP_608334.3; NM_134490.3.
DR AlphaFoldDB; Q9VWF2; -.
DR SMR; Q9VWF2; -.
DR BioGRID; 59261; 28.
DR DIP; DIP-48904N; -.
DR IntAct; Q9VWF2; 26.
DR MINT; Q9VWF2; -.
DR STRING; 7227.FBpp0074531; -.
DR PaxDb; Q9VWF2; -.
DR PRIDE; Q9VWF2; -.
DR DNASU; 32965; -.
DR EnsemblMetazoa; FBtr0074762; FBpp0074531; FBgn0087008.
DR EnsemblMetazoa; FBtr0334278; FBpp0306393; FBgn0087008.
DR EnsemblMetazoa; FBtr0334281; FBpp0306396; FBgn0087008.
DR GeneID; 32965; -.
DR KEGG; dme:Dmel_CG12238; -.
DR CTD; 32965; -.
DR FlyBase; FBgn0087008; e(y)3.
DR VEuPathDB; VectorBase:FBgn0087008; -.
DR eggNOG; KOG1512; Eukaryota.
DR GeneTree; ENSGT00940000155172; -.
DR InParanoid; Q9VWF2; -.
DR OrthoDB; 708781at2759; -.
DR PhylomeDB; Q9VWF2; -.
DR SignaLink; Q9VWF2; -.
DR BioGRID-ORCS; 32965; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 32965; -.
DR PRO; PR:Q9VWF2; -.
DR Proteomes; UP000000803; Chromosome X.
DR Bgee; FBgn0087008; Expressed in second segment of antenna (Drosophila) and 47 other tissues.
DR ExpressionAtlas; Q9VWF2; baseline and differential.
DR Genevisible; Q9VWF2; DM.
DR GO; GO:0035060; C:brahma complex; IDA:FlyBase.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0000791; C:euchromatin; IDA:UniProtKB.
DR GO; GO:0000792; C:heterochromatin; IDA:UniProtKB.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0005700; C:polytene chromosome; IDA:UniProtKB.
DR GO; GO:0005701; C:polytene chromosome chromocenter; IDA:UniProtKB.
DR GO; GO:0031213; C:RSF complex; IBA:GO_Central.
DR GO; GO:0003682; F:chromatin binding; IDA:UniProtKB.
DR GO; GO:0042393; F:histone binding; IBA:GO_Central.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0003713; F:transcription coactivator activity; IMP:UniProtKB.
DR GO; GO:0003712; F:transcription coregulator activity; IBA:GO_Central.
DR GO; GO:0003714; F:transcription corepressor activity; IMP:UniProtKB.
DR GO; GO:0006338; P:chromatin remodeling; IGI:FlyBase.
DR GO; GO:0045892; P:negative regulation of transcription, DNA-templated; IMP:UniProtKB.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0045893; P:positive regulation of transcription, DNA-templated; IMP:UniProtKB.
DR Gene3D; 3.30.40.10; -; 1.
DR InterPro; IPR011011; Znf_FYVE_PHD.
DR InterPro; IPR001965; Znf_PHD.
DR InterPro; IPR019787; Znf_PHD-finger.
DR InterPro; IPR013083; Znf_RING/FYVE/PHD.
DR SMART; SM00249; PHD; 2.
DR SUPFAM; SSF57903; SSF57903; 1.
DR PROSITE; PS01359; ZF_PHD_1; 2.
DR PROSITE; PS50016; ZF_PHD_2; 2.
PE 1: Evidence at protein level;
KW Activator; Chromosome; Cytoplasm; Metal-binding; Nucleus;
KW Reference proteome; Repeat; Repressor; Transcription;
KW Transcription regulation; Zinc; Zinc-finger.
FT CHAIN 1..2006
FT /note="Supporter of activation of yellow protein"
FT /id="PRO_0000059340"
FT ZN_FING 1694..1751
FT /note="PHD-type 1; degenerate"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00146"
FT ZN_FING 1753..1796
FT /note="PHD-type 2; degenerate"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00146"
FT REGION 1..208
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 313..347
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 458..564
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 744..794
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 821..916
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 929..1029
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1044..1082
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1099..1196
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1340..1573
FT /note="SAY"
FT REGION 1579..1685
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1887..1911
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..78
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 79..94
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 95..117
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 132..151
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 170..184
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 481..497
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 507..535
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 548..564
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 744..758
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 773..788
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 824..844
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 959..976
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 977..1029
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1099..1124
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1167..1184
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1599..1615
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1623..1685
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1887..1910
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 2006 AA; 212726 MW; B1567640FB4E5084 CRC64;
MNDLRQQQMV AATSSSGSES GTAVESAAAT STAGSAGAAG RPQSNCSANS NAKSVAASST
SEEEQRVSST SSPAQRDQQL NADRDREQEP EPQQQQQREE ALQHQHNQPG HITSTTASPP
PTLPPPTTPC DDAPSTTGAS ASASSASGEA PSAASAAGAA GGPMAATALE VESEERDGHK
IILKLSKHAN PNSNANESQP GGDERRVEPL RIQLPCGGAE GGLVAKQQDA FDADASSCSS
SCAEDEVATT LGQQLRNTPH IVPKLTIRAA NERRVGSVVP KLTIKLPENP AASGSNSNSG
SCSAAVSGAQ SAMPAKNDAH LSSLSPASAS SSSASSSSSS SSSSSLAEMQ TVPKLMIKTT
LAGSSCISSS EELPQQQQQI PKLTIKTGGG GQEHVHTVIM THDLNNAQSI PKLTIKTKSI
EMIEDEQAAK LEQQLQPLPK LTIKNLCSPK HKVRAVLEEK PPTAASKLAI PKPTPNPTPA
PMTNGGESNS SSQEFCGFSD PDADIAAPAS DDVRRNSDDM VIDDSLSKEH DPKIFHNLPP
MPASNGIASG GSKASKASKS AQSQHNVVDM VDLTSSPSPG SSPTHVPNNF TGRISPKGLL
IDCLRMQTAI GCYVPEEADS RVRPAASPNS NILLSQLTAP AKSFTTTTPK SKSSKYPQLT
ERLMANGSGT GGGSVIAAES EAVVGPLPAA NPAQSIIESI EILDTPDGSP RVAYMDEDSN
PMLNKHLQTI HKLAMDHGVE QRMDTQDNNN ENHLKRTNSE GNESPSSRLP PSKQRRLDND
ENDQQTQNCH DPARKCSESL QALPPSHRSR KQKHERILNT DLEGSLFPPT QQQSISTPDQ
NGALSSEVEG EDAKAQDPLE PATPQPPPVA TPAGTGKKRG RPKRIQNQSS PDGGGAVTPK
PGTPQEEANS AVKSRRVQLL RKRLAIDMVS VGQEQADMKA KEKESSVGVP NARVEDGLAE
TLESPKTRDH RPLRATRRTT TSTNTNLQPT PKSTRKRQSK ASVQQSQLPP PTMAQFGSSE
SESNNNNNSS ISFALSAQID LTMCSSSSST SSGAAANQQV IGGSGSSSML PPTTILSSSD
PLPDVIFQPN DFSSIMATQQ LRSSRPSSIS CGSTGGSQPD CHDEDNYTSA LDNSGDETAL
PMPPTRGRGR GRRSRGGRGR GSSSVDRAVS VGGTASTTPA TQPRAPRMSR GASAVAKAIA
MSRPRCVGGL KHQPDPERLK GLFSPSPQVF EEDTRMSADL SNSNQSMASL MEPPLTPQKQ
PDFLNNEESQ SSMVSNVSMM DSNQGQSADA ILGSALKRPK KKKMETCVAE DTDYSASSIA
EYDWPPPKGC CPSKNRDTFM IQEQVALYLG ITSFKRKYPD LPRRSVDMEE RNWLQEKGLV
SEKLCDLGIT AVWASDILDI MYADFFDKYE EYKEYIRRKH LREIEAKQKA LGLTVGAGRG
LQARDRAMLS ATKWNAYFNK SRKDERQSCM DLQTFTINQP QPRTAPTCTR LERSTSDLIR
AVAEEANIPA PPNLLPPRDY DEAFRNSDYA YPLTVVPDQF SMAYRQFEPA ELRAYPLDTA
LDKPPTDLMA QLLQAKSEAV GSDEIKTSAP APKDLGQEQS AIKSVTVTAP VRRSRRSTRQ
QTDKVRTASS SSTSSAQSVS SASSGNGSSS DTESGDESDF SSTSSCSSST GASSGAGSED
EDGNECSSSV RLSTCGVCLR SQHRNARDMP EAFIRCYTCR KRVHPSCVDM PPRMVGRVRN
YNWQCAGCKC CIKCRSSQRP GKMLYCEQCD RGYHIYCLGL RTVPDGRWSC ERCCFCMRCG
ATKPEGLPQV AALSQASGGP SANGDRSKAA RNKRLKWVHE YRIDHVTKIR EHAAMFCVPC
ARNKPAKRQS AAGAAGAAAV TPVLEATSAQ TDDSPMPSPG LTTNGGRALS PTAALSPKAA
VPVASLPPVL EATTVTTNIA GTIGRRQAGN AVNITTMQCS SSSSSNFSGN GVTEDAANVT
ATGTATAAAG APAATPIGIA PPPVVA
