SBI_STAAE
ID SBI_STAAE Reviewed; 436 AA.
AC A6QJQ7;
DT 10-FEB-2009, integrated into UniProtKB/Swiss-Prot.
DT 21-AUG-2007, sequence version 1.
DT 25-MAY-2022, entry version 80.
DE RecName: Full=Immunoglobulin-binding protein Sbi;
DE Flags: Precursor;
GN Name=sbi; OrderedLocusNames=NWMN_2317;
OS Staphylococcus aureus (strain Newman).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC Staphylococcus.
OX NCBI_TaxID=426430;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Newman;
RX PubMed=17951380; DOI=10.1128/jb.01000-07;
RA Baba T., Bae T., Schneewind O., Takeuchi F., Hiramatsu K.;
RT "Genome sequence of Staphylococcus aureus strain Newman and comparative
RT analysis of staphylococcal genomes: polymorphism and evolution of two major
RT pathogenicity islands.";
RL J. Bacteriol. 190:300-310(2008).
RN [2]
RP FUNCTION, INTERACTION WITH HOST C3 AND CFH, AND SUBCELLULAR LOCATION.
RC STRAIN=H591;
RX PubMed=19112495; DOI=10.1371/journal.ppat.1000250;
RA Haupt K., Reuter M., van den Elsen J., Burman J., Haelbich S., Richter J.,
RA Skerka C., Zipfel P.F.;
RT "The Staphylococcus aureus protein Sbi acts as a complement inhibitor and
RT forms a tripartite complex with host complement Factor H and C3b.";
RL PLoS Pathog. 4:E1000250-E1000250(2008).
RN [3]
RP FUNCTION, AND SUBCELLULAR LOCATION.
RX PubMed=21708997; DOI=10.1128/iai.05075-11;
RA Smith E.J., Visai L., Kerrigan S.W., Speziale P., Foster T.J.;
RT "The Sbi protein is a multifunctional immune evasion factor of
RT Staphylococcus aureus.";
RL Infect. Immun. 79:3801-3809(2011).
RN [4]
RP SUBCELLULAR LOCATION, AND FUNCTION.
RX PubMed=22256861; DOI=10.1111/j.1365-2958.2011.07966.x;
RA Smith E.J., Corrigan R.M., van der Sluis T., Gruendling A., Speziale P.,
RA Geoghegan J.A., Foster T.J.;
RT "The immune evasion protein Sbi of Staphylococcus aureus occurs both
RT extracellularly and anchored to the cell envelope by binding lipoteichoic
RT acid.";
RL Mol. Microbiol. 83:789-804(2012).
RN [5]
RP SUBCELLULAR LOCATION.
RC STRAIN=Newman;
RX PubMed=24434550; DOI=10.1073/pnas.1317181111;
RA Becker S., Frankel M.B., Schneewind O., Missiakas D.;
RT "Release of protein A from the cell wall of Staphylococcus aureus.";
RL Proc. Natl. Acad. Sci. U.S.A. 111:1574-1579(2014).
CC -!- FUNCTION: Plays a role in the inhibition of both the innate and
CC adaptive immune responses (PubMed:21708997). Possesses two N-terminal
CC domains that bind the Fc region of IgG and two domains that form a
CC tripartite complex with complement factors C3b and CFH. By recruiting
CC CFH and C3b, the secreted form acts as a potent complement inhibitor of
CC the alternative pathway-mediated lysis (PubMed:22256861,
CC PubMed:19112495). {ECO:0000269|PubMed:19112495,
CC ECO:0000269|PubMed:21708997, ECO:0000269|PubMed:22256861}.
CC -!- SUBUNIT: Interacts (via sbi-I and sbi-II domains) with the Fc region of
CC mammalian immunoglobulin G (IgG) proteins. Interacts (via sbi-III and
CC sbi-IV domains) with host complement C3 (PubMed:19112495). Interacts
CC (via sbi-III and sbi-IV domains) with host CFH (PubMed:19112495).
CC Interacts (via sbi-IV domain) with beta-2-glycoprotein 1/APOH (By
CC similarity). {ECO:0000250|UniProtKB:Q931F4,
CC ECO:0000269|PubMed:19112495}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:19112495,
CC ECO:0000269|PubMed:21708997, ECO:0000269|PubMed:22256861,
CC ECO:0000269|PubMed:24434550}. Cell membrane
CC {ECO:0000269|PubMed:21708997, ECO:0000269|PubMed:22256861}. Note=Occurs
CC both extracellularly and associated with the cytoplasmic membrane where
CC only the domains I and II are exposed to the extracellular media
CC (PubMed:21708997, PubMed:22256861). Membrane association occurs via
CC binding to lipoteichoic acid (PubMed:22256861).
CC {ECO:0000269|PubMed:21708997, ECO:0000269|PubMed:22256861}.
CC -!- DOMAIN: Sbi-I and sbi-II domains provide protection only when anchored
CC to the cell surface, whereas only the secreted sbi-III and sbi-IV
CC domains are biologically active. {ECO:0000269|PubMed:21708997}.
CC -!- SIMILARITY: Belongs to the immunoglobulin-binding protein Sbi family.
CC {ECO:0000305}.
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DR EMBL; AP009351; BAF68589.1; -; Genomic_DNA.
DR RefSeq; WP_000792564.1; NZ_CP023390.1.
DR AlphaFoldDB; A6QJQ7; -.
DR SMR; A6QJQ7; -.
DR EnsemblBacteria; BAF68589; BAF68589; NWMN_2317.
DR KEGG; sae:NWMN_2317; -.
DR HOGENOM; CLU_051343_0_0_9; -.
DR OMA; NAHASEQ; -.
DR PRO; PR:A6QJQ7; -.
DR Proteomes; UP000006386; Chromosome.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0019864; F:IgG binding; IEA:UniProtKB-KW.
DR Gene3D; 1.10.10.1270; -; 1.
DR InterPro; IPR009063; Ig/albumin-bd_sf.
DR InterPro; IPR021657; IgG-binding_Sbi_dom_IV.
DR InterPro; IPR003132; Protein_A_Ig-bd.
DR InterPro; IPR041909; Sbi_C3_db_domIV.
DR Pfam; PF02216; B; 2.
DR Pfam; PF11621; Sbi-IV; 1.
DR SUPFAM; SSF46997; SSF46997; 2.
PE 1: Evidence at protein level;
KW Cell membrane; IgG-binding protein; Membrane; Repeat; Secreted; Signal;
KW Virulence.
FT SIGNAL 1..29
FT /evidence="ECO:0000255"
FT CHAIN 30..436
FT /note="Immunoglobulin-binding protein Sbi"
FT /id="PRO_0000361895"
FT REPEAT 43..94
FT /note="B 1"
FT REPEAT 95..148
FT /note="B 2"
FT REPEAT 267..271
FT /note="2-1"
FT REPEAT 272..276
FT /note="2-2"
FT REPEAT 277..281
FT /note="2-3"
FT REPEAT 282..286
FT /note="2-4"
FT REPEAT 287..291
FT /note="2-5"
FT REPEAT 292..296
FT /note="2-6"
FT REPEAT 297..301
FT /note="2-7"
FT REPEAT 302..306
FT /note="2-8"
FT REGION 42..94
FT /note="Sbi-I"
FT REGION 103..153
FT /note="Sbi-II"
FT REGION 154..195
FT /note="Sbi-III"
FT REGION 196..253
FT /note="Sbi-IV"
FT REGION 267..306
FT /note="8 X 5 AA tandem repeat of P-[KQ]-[AISV]-[EKQ]-
FT [AKLSV]"
SQ SEQUENCE 436 AA; 50070 MW; 6526AE719C6CD051 CRC64;
MKNKYISKLL VGAATITLAT MISNGEAKAS ENTQQTSTKH QTTQNNYVTD QQKAFYQVLH
LKGITEEQRN QYIKTLREHP ERAQEVFSES LKDSKNPDRR VAQQNAFYNV LKNDNLTEQE
KNNYIAQIKE NPDRSQQVWV ESVQSSKAKE RQNIENADKA IKDFQDNKAP HDKSAAYEAN
SKLPKDLRDK NNRFVEKVSI EKAIVRHDER VKSANDAISK LNEKDSIENR RLAQREVNKA
PMDVKEHLQK QLDALVAQKD AEKKVAPKVE APQIQSPQIE KPKVESPKVE VPQIQSPKVE
VPQSKLLGYY QSLKDSFNYG YKYLTDTYKS YKEKYDTAKY YYNTYYKYKG AIDQTVLTVL
GSGSKSYIQP LKVDDKNGYL AKSYAQVRNY VTESINTGKV LYTFYQNPTL VKTAIKAQET
ASSIKNTLSN LLSFWK
