SBNO_CAEEL
ID SBNO_CAEEL Reviewed; 1866 AA.
AC O01737; D5LYH1;
DT 16-OCT-2019, integrated into UniProtKB/Swiss-Prot.
DT 25-JAN-2012, sequence version 3.
DT 03-AUG-2022, entry version 158.
DE RecName: Full=Protein strawberry notch homolog {ECO:0000305};
DE AltName: Full=Lethal protein 765 {ECO:0000312|WormBase:F20H11.2};
DE AltName: Full=Notch signaling pathway homolog 1 {ECO:0000303|PubMed:20230814};
GN Name=let-765 {ECO:0000303|PubMed:20230814, ECO:0000312|WormBase:F20H11.2};
GN Synonyms=let-745 {ECO:0000312|WormBase:F20H11.2},
GN nsh-1 {ECO:0000303|PubMed:20230814, ECO:0000312|WormBase:F20H11.2};
GN ORFNames=F20H11.2 {ECO:0000312|WormBase:F20H11.2};
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239 {ECO:0000312|Proteomes:UP000001940};
RN [1] {ECO:0000305}
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE
RP SPECIFICITY, DEVELOPMENTAL STAGE, DISRUPTION PHENOTYPE, AND MUTAGENESIS OF
RP 381-ARG--PRO-1866 AND 941-TRP--PRO-1866.
RX PubMed=20230814; DOI=10.1016/j.ydbio.2010.03.004;
RA Simms C.L., Baillie D.L.;
RT "A strawberry notch homolog, let-765/nsh-1, positively regulates lin-3/egf
RT expression to promote RAS-dependent vulval induction in C. elegans.";
RL Dev. Biol. 341:472-485(2010).
RN [2] {ECO:0000312|Proteomes:UP000001940}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2 {ECO:0000312|Proteomes:UP000001940};
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
CC -!- FUNCTION: Transcriptional activator that functions upstream of the let-
CC 60/Ras and let-23/EGFR signaling pathways to positively regulate lin-3
CC expression and thereby promote vulval induction (PubMed:20230814).
CC Plays a role in excretory duct development (PubMed:20230814). Plays a
CC role in male tail development (PubMed:20230814).
CC {ECO:0000269|PubMed:20230814}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:20230814}.
CC -!- TISSUE SPECIFICITY: Expressed in the somatic gonad, neurons, hypodermal
CC cells, seam cells, the excretory system, and intestinal cells (at
CC protein level). {ECO:0000269|PubMed:20230814}.
CC -!- DEVELOPMENTAL STAGE: Expressed in most cells of the embryo beginning
CC from the 100-cell stage (at protein level) (PubMed:20230814). Expressed
CC throughout larval development in the excretory system, anterior neurons
CC and the hypodermis (at protein level) (PubMed:20230814). Expressed in
CC vulval precursor cells and their descendants during the mid-larval
CC stages and is highly expressed in the somatic gonad from the L3 larval
CC stage to adulthood (at protein level) (PubMed:20230814).
CC {ECO:0000269|PubMed:20230814}.
CC -!- DISRUPTION PHENOTYPE: RNAi-mediated knockdown results in embryonic or
CC larval lethality (PubMed:20230814). Surviving animals exhibit slow
CC growth, gonad migration defects, vulval induction defects and reduced
CC lin-3 expression (PubMed:20230814). Larvae that develop into adults are
CC sterile, and only few produce embryos (PubMed:20230814). RNAi-mediated
CC knockdown in males results in tail defects including underdeveloped
CC tails, spicule defects and missing rays (PubMed:20230814). RNAi-
CC mediated knockdown in a lin-3 e1417 mutant background abolishes vulval
CC induction (PubMed:20230814). RNAi-mediated knockdown in a let-23 sa62
CC gain of function mutant background partially suppresses the multivulva
CC phenotype in the let-23 mutant (PubMed:20230814). RNAi-mediated
CC knockdown in a let-60 n1046 gain of function mutant background
CC suppresses the multivulva phenotype in the let-60 mutant
CC (PubMed:20230814). {ECO:0000269|PubMed:20230814}.
CC -!- SIMILARITY: Belongs to the SBNO family. {ECO:0000305}.
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DR EMBL; GU997622; ADF27719.1; -; mRNA.
DR EMBL; BX284603; CCD69779.1; -; Genomic_DNA.
DR RefSeq; NP_498455.2; NM_066054.4.
DR AlphaFoldDB; O01737; -.
DR STRING; 6239.F20H11.2; -.
DR EPD; O01737; -.
DR PaxDb; O01737; -.
DR PeptideAtlas; O01737; -.
DR EnsemblMetazoa; F20H11.2.1; F20H11.2.1; WBGene00002889.
DR GeneID; 175934; -.
DR KEGG; cel:CELE_F20H11.2; -.
DR UCSC; F20H11.2; c. elegans.
DR CTD; 175934; -.
DR WormBase; F20H11.2; CE45139; WBGene00002889; let-765.
DR eggNOG; KOG1513; Eukaryota.
DR GeneTree; ENSGT00940000167724; -.
DR HOGENOM; CLU_000212_2_1_1; -.
DR InParanoid; O01737; -.
DR OMA; QWFGQDY; -.
DR OrthoDB; 141580at2759; -.
DR PhylomeDB; O01737; -.
DR PRO; PR:O01737; -.
DR Proteomes; UP000001940; Chromosome III.
DR Bgee; WBGene00002889; Expressed in embryo and 3 other tissues.
DR GO; GO:0005634; C:nucleus; IDA:WormBase.
DR GO; GO:0031490; F:chromatin DNA binding; IBA:GO_Central.
DR GO; GO:0042393; F:histone binding; IBA:GO_Central.
DR GO; GO:0045138; P:nematode male tail tip morphogenesis; IMP:WormBase.
DR GO; GO:0042660; P:positive regulation of cell fate specification; IMP:WormBase.
DR GO; GO:0046579; P:positive regulation of Ras protein signal transduction; IGI:WormBase.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IBA:GO_Central.
DR GO; GO:0072327; P:vulval cell fate specification; IMP:WormBase.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR026937; SBNO_Helicase_C_dom.
DR InterPro; IPR026741; SNO.
DR InterPro; IPR039187; SNO_AAA.
DR PANTHER; PTHR12706; PTHR12706; 1.
DR Pfam; PF13872; AAA_34; 1.
DR Pfam; PF13871; Helicase_C_4; 1.
DR SUPFAM; SSF52540; SSF52540; 2.
PE 1: Evidence at protein level;
KW Nucleus; Reference proteome; Transcription; Transcription regulation.
FT CHAIN 1..1866
FT /note="Protein strawberry notch homolog"
FT /id="PRO_0000448279"
FT REGION 19..63
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 132..151
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 156..253
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 561..581
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1112..1308
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 132..146
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 159..240
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 563..577
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1112..1141
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1161..1187
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1194..1215
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1248..1277
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1278..1293
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MUTAGEN 381..1866
FT /note="Missing: In s2575; larvae arrest during the L2
FT larval stage."
FT /evidence="ECO:0000269|PubMed:20230814"
FT MUTAGEN 941..1866
FT /note="Missing: In s2630; larvae arrest during the L1 to L2
FT transition."
FT /evidence="ECO:0000269|PubMed:20230814"
SQ SEQUENCE 1866 AA; 205252 MW; 80A37ACD4F2E716B CRC64;
MDDILSAALA ESGLDFLCQQ SSPTPSTSGS IHDDAGQSFS NNTHTPSVSQ FFDETSNDSH
SSSAYYTPMA TPFVSTEDGG VPTSFFGMDE EDGGCTIMTT AGTSGSNNID GIEDAGGGMY
YPHVKVIPRK HTAPTVNQSE PSTPTVTIVP KKEDPLFETN TADSPTPSGD TSTTASYEGN
DGLEDQETTS DRQNPMFVQT ARSTDGRLDT PSTSATVSPH ITSSLTQRSH TSSPASSASE
GTVVPPRKKG LPITTGSIVK RTVQTKDGLQ TQYLKAFVNE NGEKIYKLLS PVAASAVARG
TLPPGMGRGG STIGRGGTMV NKNGERLMVV KNHVGPNGQM LVKRMVSPAG TRIVANGGQG
RGQPIYRAVD GSNGPTHLLR RTTTTGQPTR GAPVGMAARH AVRGGTVYGG GNGYRVNLVG
RGTGGSTMVH HQPLNRISSQ RSVAPVGRVL NRGALRNGAQ QPLHVSTSSP AFHYMEEQPS
PTTNGMVIQA KTPGAGVIQA RHMQSQQSFP SGGPARVLMN RSSTNAGLSR MVGGGYDQQL
PTAPNGRLMI PSTAVRVPGS GMASPRLQTT PQPLTKSQKA KDEMKMAYQV GREEALQQRR
NDLEDDEENL GYAETYSEYT PAKLRSGMAH PDSVVESASL SSVSPPDVKY QISIPEYLID
MGHISALQLE AVIYACQMHE RRMPSGERYG YLIGDGAGVG KGRTVACIIF ENYLQGRKRA
IWLSVSSDLK FDAERDLRDC GAPNIPVYAL NKMKYAKISG KENGSIKKGV MFATYTSLIG
ECRGAKSRKY RSRISQLIQW FGQDYDGVII LDECHRAKNL VPTAGAKPTK TGRMVLELQK
ALPNARVVYA SATGATEPRN MAYMTRLGLW GERQAFPEFH DFISAVERRG VGAMEIVAMD
MKQRGLYLAR QLSFRGVSFA VQEVQLSSEF VKMYDAAVKL WMEARRQFQT VIETMDEEER
STCKTVWGQF WACHQRFFKY LCIAAKVDTC VQLSREAIKA KKCVVIGLQS TGESATLETL
EEMGGELNEF VSTAKTVLYG LIDKHFPTDA SFSMGDRDIF KDFDDFERPA KRRKTRETLS
FLGDVGFDTW TGVTTGMGGR VGDGVTKNIT RGLSGIGRSS MSSSTGNTNN EDANSTTSES
SDGSDDEVEN DMISENGGES GDLESAREEA EGARTLEDGE QDEWVKALLA EAESSSDDSD
EEVVKDEDED EEAESKSGET HEQEEEFNPF MCDFTNDDPW AHNQQIVEDT PQKDRKAKKR
KRDEEEAERL REKVRKREER REKKRRRAIR RAEREKQRRN EELQARGSAT DFITSSRICG
NGSGEQDDIN PMLIKTELLA AVERLAPSLP ANTLDQLIDE MGGPEYVAEM TGRRGHMVTS
ETGDVMYQRR NANAEVSLEL INMEEKEKFM RGEKLIAIIS EAASSGISLQ SDRRAINKRR
RVHITLELPW SADKAIQQFG RTHRSNQVSG PEYVFLISEL AGEKRFASIV AKRLESLGAL
THGDRRATET RDLSQFNMDN KYGRVALDTL LKTVIGQAGT PLIDPPKDYK AGEFFEDMRL
YMEGVGLLAK NKTGQYTIEK EAATIPKFLN RILGLPVHAQ NSLFHYFSEI VAELIAQSKH
DGTYDTGIMD LGTGDDQVRK LETRVFTGRV DNGSFRVEIH KIGVERGVSW EEAMELHKEH
SNDDDGFYIC HPGGANTANT KKVAALVYGI GKIRMDNGAR LYAITRPSTG RSPKLMTMAD
LSKRFHKVSI DEAKEVWKQQ YDSAANMCQH NYVYGKCRTE SNGTYCEVGR RTRTYFVLSG
SVLSVWPIVE EVLAGSDRKS SRMQVIRVRT EQDQKIVGLL VLPTHVRHLV QQLETHCGRS
YVKTEP
