SBNO_DROME
ID SBNO_DROME Reviewed; 1653 AA.
AC A8JUV0; A8JUU9; O01940; Q8MZI4; Q95S83; Q9VYF7;
DT 08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT 16-MAR-2016, sequence version 2.
DT 03-AUG-2022, entry version 101.
DE RecName: Full=Protein strawberry notch;
GN Name=sno {ECO:0000312|FlyBase:FBgn0265630};
GN ORFNames=CG44436 {ECO:0000312|FlyBase:FBgn0265630};
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1] {ECO:0000305, ECO:0000312|EMBL:AAB54001.1}
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A), FUNCTION, SUBCELLULAR LOCATION,
RP TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, AND DISRUPTION PHENOTYPE.
RX PubMed=9171377; DOI=10.1101/gad.11.10.1341;
RA Majumdar A., Nagaraj R., Banerjee U.;
RT "strawberry notch encodes a conserved nuclear protein that functions
RT downstream of Notch and regulates gene expression along the developing wing
RT margin of Drosophila.";
RL Genes Dev. 11:1341-1353(1997).
RN [2] {ECO:0000312|EMBL:ABW09405.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley {ECO:0000269|PubMed:10731132};
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [3] {ECO:0000305, ECO:0000312|EMBL:ABW09405.1}
RP GENOME REANNOTATION, AND ALTERNATIVE SPLICING.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [4] {ECO:0000305, ECO:0000312|EMBL:AAL28466.1, ECO:0000312|EMBL:AAM27487.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM A), NUCLEOTIDE SEQUENCE
RP [LARGE SCALE MRNA] OF 1018-1653 (ISOFORM A/B), AND NUCLEOTIDE SEQUENCE
RP [LARGE SCALE MRNA] OF 1-187.
RC STRAIN=Berkeley {ECO:0000312|EMBL:AAM27487.1};
RC TISSUE=Ovary {ECO:0000269|PubMed:12537569};
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [5]
RP FUNCTION, AND DEVELOPMENTAL STAGE.
RX PubMed=8287794; DOI=10.1242/dev.119.2.377;
RA Coyle-Thompson C.A., Banerjee U.;
RT "The strawberry notch gene functions with Notch in common developmental
RT pathways.";
RL Development 119:377-395(1993).
RN [6]
RP FUNCTION, INTERACTION WITH VG, AND TISSUE SPECIFICITY.
RX PubMed=11731237; DOI=10.1016/s0925-4773(01)00539-1;
RA Nagel A.C., Wech I., Preiss A.;
RT "Scalloped and strawberry notch are target genes of Notch signaling in the
RT context of wing margin formation in Drosophila.";
RL Mech. Dev. 109:241-251(2001).
RN [7]
RP FUNCTION, INTERACTION WITH SU(H), AND TISSUE SPECIFICITY.
RX PubMed=12230979; DOI=10.1016/s0092-8674(02)00875-9;
RA Tsuda L., Nagaraj R., Zipursky S.L., Banerjee U.;
RT "An EGFR/Ebi/Sno pathway promotes delta expression by inactivating
RT Su(H)/SMRTER repression during inductive notch signaling.";
RL Cell 110:625-637(2002).
RN [8]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-24; SER-26; SER-929 AND
RP SER-931, AND IDENTIFICATION BY MASS SPECTROMETRY.
RC TISSUE=Embryo;
RX PubMed=18327897; DOI=10.1021/pr700696a;
RA Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P.;
RT "Phosphoproteome analysis of Drosophila melanogaster embryos.";
RL J. Proteome Res. 7:1675-1682(2008).
CC -!- FUNCTION: Notch pathway component, may contribute to the specificity
CC between lateral and inductive Notch signaling pathways in the wing
CC disk. Required during many developmental stages including oogenesis,
CC embryogenesis and imaginal development of the eye, wing and leg. Ebi
CC and sno regulate EGFR-dependent Delta transcription in the developing
CC eye, by antagonizing a repressor function of Suppressor of Hairless
CC (Su(H)). They are required in the R-cells for normal cone cell
CC development. {ECO:0000269|PubMed:11731237, ECO:0000269|PubMed:12230979,
CC ECO:0000269|PubMed:8287794, ECO:0000269|PubMed:9171377}.
CC -!- SUBUNIT: Interacts with vg for function in the wing disk. Interacts
CC with Su(H) for function in the eye disk. {ECO:0000269|PubMed:11731237,
CC ECO:0000269|PubMed:12230979}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:9171377}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=B {ECO:0000312|FlyBase:FBgn0265630};
CC IsoId=A8JUV0-4; Sequence=Displayed;
CC Name=A {ECO:0000312|FlyBase:FBgn0265630};
CC IsoId=A8JUV0-3; Sequence=VSP_058149;
CC -!- TISSUE SPECIFICITY: At stage 8, when the formation of the midline
CC precursor cells depends on Notch signaling, high level of expression is
CC seen in the midline precursor cells and a lower level in the
CC surrounding epidermal cells. Between stages 11 and 14, expression is
CC uniform throughout the epidermis, and at stage 16, high level of
CC expression is restricted to the central nervous system. Expressed in
CC the larval leg, wing and eye imaginal disks. Expression is over the
CC wing disk and accumulates within the pleural region.
CC {ECO:0000269|PubMed:11731237, ECO:0000269|PubMed:12230979,
CC ECO:0000269|PubMed:9171377}.
CC -!- DEVELOPMENTAL STAGE: Expressed both maternally and zygotically. First
CC detected at the syncytial blastoderm stage.
CC {ECO:0000269|PubMed:8287794, ECO:0000269|PubMed:9171377}.
CC -!- DISRUPTION PHENOTYPE: Loss of wingless, vestigial, cut, and E(spl)-m8
CC expression at the dorsal/ventral (D/V) boundary of the wing disk
CC causing improper boundary specification. This causes defective wing
CC pouch development and consequent thickening of wing veins.
CC {ECO:0000269|PubMed:9171377}.
CC -!- SIMILARITY: Belongs to the SBNO family. {ECO:0000255}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAL28466.1; Type=Miscellaneous discrepancy; Note=Intron retention.; Evidence={ECO:0000305};
CC Sequence=AAM27487.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR EMBL; U95760; AAB54001.1; -; mRNA.
DR EMBL; AE014298; AAF48240.1; -; Genomic_DNA.
DR EMBL; AE014298; ABW09405.1; -; Genomic_DNA.
DR EMBL; AY060918; AAL28466.1; ALT_SEQ; mRNA.
DR EMBL; AY102658; AAM27487.1; ALT_INIT; mRNA.
DR PIR; T13847; T13847.
DR RefSeq; NP_001096967.1; NM_001103497.2. [A8JUV0-4]
DR RefSeq; NP_511144.2; NM_078589.4. [A8JUV0-3]
DR AlphaFoldDB; A8JUV0; -.
DR BioGRID; 58658; 11.
DR IntAct; A8JUV0; 4.
DR STRING; 7227.FBpp0111762; -.
DR iPTMnet; A8JUV0; -.
DR PaxDb; A8JUV0; -.
DR PRIDE; A8JUV0; -.
DR DNASU; 32273; -.
DR EnsemblMetazoa; FBtr0340488; FBpp0309424; FBgn0265630. [A8JUV0-3]
DR EnsemblMetazoa; FBtr0340489; FBpp0309425; FBgn0265630. [A8JUV0-4]
DR GeneID; 32273; -.
DR KEGG; dme:Dmel_CG44436; -.
DR CTD; 20622; -.
DR FlyBase; FBgn0265630; sno.
DR VEuPathDB; VectorBase:FBgn0265630; -.
DR eggNOG; KOG1513; Eukaryota.
DR GeneTree; ENSGT00940000167724; -.
DR InParanoid; A8JUV0; -.
DR OMA; VSQMWMN; -.
DR BioGRID-ORCS; 32273; 1 hit in 3 CRISPR screens.
DR GenomeRNAi; 32273; -.
DR PRO; PR:A8JUV0; -.
DR Proteomes; UP000000803; Chromosome X.
DR Bgee; FBgn0265630; Expressed in brain and 29 other tissues.
DR Genevisible; A8JUV0; DM.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0031490; F:chromatin DNA binding; IBA:GO_Central.
DR GO; GO:0042393; F:histone binding; IBA:GO_Central.
DR GO; GO:0008587; P:imaginal disc-derived wing margin morphogenesis; IMP:FlyBase.
DR GO; GO:0007219; P:Notch signaling pathway; IMP:UniProtKB.
DR GO; GO:0042461; P:photoreceptor cell development; IMP:FlyBase.
DR GO; GO:0045747; P:positive regulation of Notch signaling pathway; IGI:FlyBase.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IMP:FlyBase.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IBA:GO_Central.
DR GO; GO:0048190; P:wing disc dorsal/ventral pattern formation; IMP:UniProtKB.
DR Gene3D; 3.40.50.300; -; 2.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR026937; SBNO_Helicase_C_dom.
DR InterPro; IPR026741; SNO.
DR InterPro; IPR039187; SNO_AAA.
DR PANTHER; PTHR12706; PTHR12706; 2.
DR Pfam; PF13872; AAA_34; 1.
DR Pfam; PF13871; Helicase_C_4; 1.
DR SUPFAM; SSF52540; SSF52540; 2.
PE 1: Evidence at protein level;
KW Alternative splicing; Coiled coil; Notch signaling pathway; Nucleus;
KW Phosphoprotein; Reference proteome.
FT CHAIN 1..1653
FT /note="Protein strawberry notch"
FT /id="PRO_0000328929"
FT REGION 1..46
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 190..211
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 237..265
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 317..345
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 883..1043
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 1064..1125
FT /evidence="ECO:0000255"
FT COMPBIAS 14..36
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 883..908
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 921..950
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 954..975
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 981..995
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1010..1027
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 24
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MOD_RES 26
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MOD_RES 929
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MOD_RES 931
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT VAR_SEQ 188..294
FT /note="AMGSPAARSSGNAGTTGSSQGGAIGSTSSYLNSLTTNELMNLAAYVAAKGSN
FT APPPPPPSTAANSVRHSPTGGIPNPGGNFFGGSAAASTSASAANFNMAASLLAQM ->
FT V (in isoform A)"
FT /evidence="ECO:0000305"
FT /id="VSP_058149"
FT CONFLICT 956
FT /note="A -> T (in Ref. 1; AAB54001)"
FT /evidence="ECO:0000305"
FT CONFLICT 1422
FT /note="V -> M (in Ref. 1; AAB54001)"
FT /evidence="ECO:0000305"
FT CONFLICT 1499
FT /note="S -> R (in Ref. 1; AAB54001)"
FT /evidence="ECO:0000305"
FT CONFLICT 1609
FT /note="S -> I (in Ref. 4; AAM27487)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 1653 AA; 180376 MW; DA0549340FE516BE CRC64;
MTSKKRKTLL DADDDNDNFD EDDSGSDFDD DEDPDQIEVP GGGRDLNTAV TYAQNIRSGV
GVAPKGGIPI PISGAKIVVG NNKIKPISLL RINNNNNNIV TSVNNRNNNN IISTNGSSNN
NNNSINNNSQ IIKTTTVTTT PTTVGATPTV GGVALGGKLT VIPIAGRNVA LDNNLSNMPK
KLNNMVTAMG SPAARSSGNA GTTGSSQGGA IGSTSSYLNS LTTNELMNLA AYVAAKGSNA
PPPPPPSTAA NSVRHSPTGG IPNPGGNFFG GSAAASTSAS AANFNMAASL LAQMSYAGGA
SQIRALKVAG NIGGVGNNQK PPPIATTPGS GGPAGGAPGS GVKGNNSMME AVQKLIAMNP
EYLTSGIPNT VFQMFMQSMQ RPQATPSPNQ PMNPGAMVTS AAAAAAHASA VAYVQQEEDE
VDYEEMGVAE TYADYWPAKL KLGKKHPDAV VETASLSSVE PCDVYYKLSL PLETINSGHL
SALQLESITY ASQAHDHLLP DGSRAGFLIG DGAGVGKGRT IAGIIYENYL KGRKKALWIS
VSNDLKYDAE RDLSDIGATR IEVHALNKFK YAKISSDVNN NCKRGVIFST YSALIGESNN
KTGKYRSRFR QLLQWCGEDF EGLIIFDECH KAKNLCPVGS GKPTKTGQTV LELQQKLPKA
RVVYASATGA SEPKNMAYMV RLGLWGQGTA FGNFNDFITA VERRGVGAME IVAMDMKLRG
MYIARQLSFK GVSFKIEEVP LSKEFRKIYD QSVELWVEAM QKFTEAAELI DAESRMKKTM
WGQFWSSHQR FFKYLCIAAK VNHAVLVARE SIKYGKCVVI GLQSTGEART LDQLERDDGE
LTDFVSTAKG VFQSFVERHF PAPDRNRINR ILGLYDETPS LSSVADSTSS LSNNSNITTA
AGKRKGSNNN DNRSTKIKKK KRSGSWECSD SEDENTDMKR NRKRDGGNSN SDSDEANSDD
DLKSDIDDED EDHDVDSDQR SVASDASSDF NPFFSGSDSD IDPWVNARSK KSKKAQKKSK
KKVKKEKTKK EITTSSATDP SGSTAMSATV VAALNAVKNR KSQLSTQDKI QDLLQKKQEL
KGTVTPVGVN GVKLNYGPPP KDAIERACTM KEELLRKIER LGARLPPNTL DQLIDELGGP
DNVAEMTGRR GRVVQTDDGS IQYESRTESD VPLETLNITE KQRFMDGEKD VAIISEAASS
GISLQSDRRV FNQRRRVHIT LELPWSADRA IQQFGRTHRS NQVNAPEYIF LISDLAGERR
FASTVAKRLE SLGALTHGDR RATETRDLSQ FNIDNKYGRQ ALETVMRTIM GYESPLVPPP
TDYSGEFFKD IAGALVGVGI IVNSESHPGV LSLDKDYNNI SKFLNRILGC PVDLQNRLFK
YFTDTMTAII QQAKRGGRFD LGILDLGAAG ENVTRVRLIR FVRKHATGVA PTEMHTVRVE
RGMIWQEAID KYADLFNENE GFYLSHQLRN QKRTAIMVVI LESRNSSSTS STTDLDSGSK
KKKTHSKKEI MCQIYRPNTG LQVRHESLFE LEKKYRKVAS EEAEPHWTEQ YDASVNTCSH
AYWNGNCRNV SLGNDCEVGL RQRLYHVLAG SVLSVWGRVE HILNTRSNSK MQVIRMKTTE
GEKIVGTLIP KSCFEPLVAD LRSDSEKQEE FNY
