SBP2_MOUSE
ID SBP2_MOUSE Reviewed; 472 AA.
AC Q63836; Q8R1T6;
DT 15-JUL-1999, integrated into UniProtKB/Swiss-Prot.
DT 29-MAY-2007, sequence version 2.
DT 03-AUG-2022, entry version 143.
DE RecName: Full=Selenium-binding protein 2;
DE AltName: Full=56 kDa acetaminophen-binding protein;
DE Short=AP56;
GN Name=Selenbp2; Synonyms=Lpsb2;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=BALB/cJ; TISSUE=Liver;
RX PubMed=8453708; DOI=10.1093/carcin/14.3.335;
RA Lanfear J., Fleming J., Walker M., Harrison P.;
RT "Different patterns of regulation of the genes encoding the closely related
RT 56 kDa selenium- and acetaminophen-binding proteins in normal tissues and
RT during carcinogenesis.";
RL Carcinogenesis 14:335-340(1993).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=FVB/N; TISSUE=Liver;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP PROTEIN SEQUENCE OF 175-189; 196-220; 228-242; 290-296; 334-343 AND
RP 399-408.
RX PubMed=1540179; DOI=10.1016/0006-291x(92)91881-p;
RA Pumford N.R., Martin B.M., Hinson J.A.;
RT "A metabolite of acetaminophen covalently binds to the 56 kDa selenium
RT binding protein.";
RL Biochem. Biophys. Res. Commun. 182:1348-1355(1992).
RN [4]
RP PROTEIN SEQUENCE OF 101-113; 227-230 AND 269-276, AND IDENTIFICATION BY
RP MASS SPECTROMETRY.
RX PubMed=16532517; DOI=10.1002/elps.200500703;
RA Mattow J., Demuth I., Haeselbarth G., Jungblut P.R., Klose J.;
RT "Selenium-binding protein 2, the major hepatic target for acetaminophen,
RT shows sex differences in protein abundance.";
RL Electrophoresis 27:1683-1691(2006).
RN [5]
RP PHOSPHORYLATION AT SER-467.
RX PubMed=15378723; DOI=10.1002/rcm.1604;
RA Jin W.-H., Dai J., Zhou H., Xia Q.-C., Zou H.-F., Zeng R.;
RT "Phosphoproteome analysis of mouse liver using immobilized metal affinity
RT purification and linear ion trap mass spectrometry.";
RL Rapid Commun. Mass Spectrom. 18:2169-2176(2004).
RN [6]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Liver;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Selenium- and acetaminophen-binding protein which may be
CC involved in the sensing of reactive xenobiotics in the cytoplasm. May
CC be involved in intra-Golgi protein transport (By similarity).
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}. Cytoplasm, cytosol.
CC Membrane {ECO:0000250}; Peripheral membrane protein {ECO:0000250}.
CC Note=May associate with Golgi membrane. May associate with the membrane
CC of autophagosomes (By similarity). {ECO:0000250}.
CC -!- TISSUE SPECIFICITY: Mainly expressed in liver.
CC -!- PTM: The N-terminus is blocked. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the selenium-binding protein family.
CC {ECO:0000305}.
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DR EMBL; S56599; AAB25841.2; -; mRNA.
DR EMBL; BC024106; AAH24106.1; -; mRNA.
DR CCDS; CCDS38539.1; -.
DR RefSeq; NP_062287.1; NM_019414.2.
DR AlphaFoldDB; Q63836; -.
DR SMR; Q63836; -.
DR BioGRID; 203157; 13.
DR IntAct; Q63836; 2.
DR MINT; Q63836; -.
DR STRING; 10090.ENSMUSP00000088358; -.
DR iPTMnet; Q63836; -.
DR PhosphoSitePlus; Q63836; -.
DR SwissPalm; Q63836; -.
DR REPRODUCTION-2DPAGE; Q63836; -.
DR jPOST; Q63836; -.
DR MaxQB; Q63836; -.
DR PaxDb; Q63836; -.
DR PeptideAtlas; Q63836; -.
DR PRIDE; Q63836; -.
DR ProteomicsDB; 256599; -.
DR DNASU; 20342; -.
DR GeneID; 20342; -.
DR KEGG; mmu:20342; -.
DR UCSC; uc008qgx.1; mouse.
DR CTD; 20342; -.
DR MGI; MGI:104859; Selenbp2.
DR eggNOG; KOG0918; Eukaryota.
DR InParanoid; Q63836; -.
DR OrthoDB; 483905at2759; -.
DR PhylomeDB; Q63836; -.
DR TreeFam; TF315241; -.
DR BioGRID-ORCS; 20342; 3 hits in 40 CRISPR screens.
DR ChiTaRS; Selenbp2; mouse.
DR PRO; PR:Q63836; -.
DR Proteomes; UP000000589; Unplaced.
DR RNAct; Q63836; protein.
DR GO; GO:0005829; C:cytosol; IEA:UniProtKB-SubCell.
DR GO; GO:0001650; C:fibrillar center; ISO:MGI.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005730; C:nucleolus; ISO:MGI.
DR GO; GO:0018549; F:methanethiol oxidase activity; ISO:MGI.
DR GO; GO:0008430; F:selenium binding; IEA:InterPro.
DR GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR InterPro; IPR008826; Se-bd.
DR PANTHER; PTHR23300; PTHR23300; 1.
DR Pfam; PF05694; SBP56; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; Direct protein sequencing; Membrane; Nucleus; Phosphoprotein;
KW Protein transport; Reference proteome; Selenium; Transport.
FT CHAIN 1..472
FT /note="Selenium-binding protein 2"
FT /id="PRO_0000174635"
FT MOD_RES 467
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:15378723"
FT CONFLICT 100
FT /note="I -> M (in Ref. 1; AAB25841)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 472 AA; 52610 MW; 923BEBCDC4657511 CRC64;
MATKCTKCGP GYPTPLEAMK GPREEIVYLP CIYRNTGTEA PDYLATVDVD PKSPQYSQVI
HRLPMPYLKD ELHHSGWNTC SSCFGDSTKS RNKLILPGLI SSRIYVVDVG SEPRAPKLHK
VIEASEIQAK CNVSNTHTSH CLASGEVMVN TLGDLQGNGK GSFVLLDGET FEVKGTWEKP
GGASPMGYDF WYQPRHNVMV STEWAAPNVF KDGFNPAHVE AGLYGSRIFV WDWQRHEIIQ
TLQMTDGLIP LEIRFLHDPS ATQGFVGCAL SSNIQRFYKN EEGTWSVEKV IQVPSKKVKG
WMLPEMPGLI TDILLSLDDR FLYFSNWLHG DIRQYDISNP QKPRLTGQIF LGGSIVRGGS
VQVLEDQELT CQPEPLVVKG KRIPGGPQMI QLSLDGKRLY ATTSLYSDWD KQFYPDLIRE
GSVMLQVDVD TVNGGLKLNP NFLVDFGKEP LGPALAHELR YPGGDCSSDI WI
