ID   SBP65_PEA               Reviewed;         551 AA.
AC   Q41060;
DT   01-JUL-2008, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   03-AUG-2022, entry version 50.
DE   RecName: Full=Seed biotin-containing protein SBP65;
DE   AltName: Full=Seed biotinylated protein of 65 kDa;
GN   Name=SBP65;
OS   Pisum sativum (Garden pea).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC   NPAAA clade; Hologalegina; IRL clade; Fabeae; Pisum.
OX   NCBI_TaxID=3888;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 17-27; 29-35; 37-49; 62-74;
RP   93-125 AND 129-146, INDUCTION, TISSUE SPECIFICITY, AND BIOTINYLATION AT
RP   LYS-103.
RC   STRAIN=cv. Douce Provence;
RX   PubMed=7948875; DOI=10.1007/bf00039537;
RA   Duval M., DeRose R.T., Job C., Faucher D., Douce R., Job D.;
RT   "The major biotinyl protein from Pisum sativum seeds covalently binds
RT   biotin at a novel site.";
RL   Plant Mol. Biol. 26:265-273(1994).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=cv. Alaska;
RX   PubMed=9349282; DOI=10.1023/a:1005836405211;
RA   Dehaye L., Duval M., Viguier D., Yaxley J., Job D.;
RT   "Cloning and expression of the pea gene encoding SBP65, a seed-specific
RT   biotinylated protein.";
RL   Plant Mol. Biol. 35:605-621(1997).
CC   -!- FUNCTION: May serve as a biotin source for several growth-limiting
CC       enzymes that are necessary during seed development and the subsequent
CC       germination stages, and thus may play some roles in determining seed
CC       germination capacity. {ECO:0000250}.
CC   -!- TISSUE SPECIFICITY: Expressed in dry mature seeds.
CC       {ECO:0000269|PubMed:7948875}.
CC   -!- INDUCTION: Induced by abscisic acid. {ECO:0000269|PubMed:7948875}.
CC   -!- SIMILARITY: Belongs to the seed biotin-containing protein SBP65 family.
CC       {ECO:0000305}.
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DR   EMBL; X75880; CAA53474.1; -; mRNA.
DR   EMBL; U49977; AAC49857.1; -; Genomic_DNA.
DR   PIR; S52657; S52657.
DR   AlphaFoldDB; Q41060; -.
DR   PRIDE; Q41060; -.
DR   EnsemblPlants; Psat5g275760.1; Psat5g275760.1.cds; Psat5g275760.
DR   Gramene; Psat5g275760.1; Psat5g275760.1.cds; Psat5g275760.
PE   1: Evidence at protein level;
KW   Biotin; Coiled coil; Direct protein sequencing; Germination.
FT   CHAIN           1..551
FT                   /note="Seed biotin-containing protein SBP65"
FT                   /id="PRO_0000342635"
FT   REGION          1..29
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          197..265
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          518..551
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COILED          141..211
FT                   /evidence="ECO:0000255"
FT   COMPBIAS        197..211
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        220..265
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        518..544
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOD_RES         103
FT                   /note="N6-biotinyllysine; atypical"
FT                   /evidence="ECO:0000269|PubMed:7948875"
SQ   SEQUENCE   551 AA;  59554 MW;  66146AE38464A08C CRC64;
     MASEQLSRRE NITTERKIQN AEDSVPQRTT HFELRETHEL GPNFQSLPRN ENQAYLDRGA
     RAPLSANVSE SYLDRARVPL NANIPEHRVR EKEDFGGVRD MGKFQMESKG GNKSLAEDRE
     TLDTRSRMVT GTPHIKEASG KGQVVEERER ARERAMEEEE KRLTMEEISK YRNQAQQSAL
     EALSAAQEKY ERAKQATNET LRNTTQAAQE KGEAAQAKDA TFEKTQQGYE MTGDTVSNSA
     RTASEKAAQA KNTTLGKTQQ GYEATRDTVS NAARTAAEYA TPAAEKARCV AVQAKDVTLE
     TGKTAAEKAK CAAEIAAKVA VDLKEKATVA GWTASHYATQ LTVDGTRAAA NAVEGAVGYV
     APKASELAAK SVETVKGLAA SAGETAKEFT ARKKEESWRE YEAKRASQLQ EGEEILPSTG
     GIGKVLPSGE RTQAQGTNLQ EKVQGKGSDI LGAVTETVSD IGSSMIKPID NANTKVKEHG
     GTTITPKGQD AGGVLDAIGE TIAEIAHTTK VIVVGEDDEV EKSMQKNIGS DSHSLDRAKH
     EGYRAPKNNV S