ID   SBP65_SOYBN             Reviewed;         643 AA.
AC   Q39846;
DT   01-JUL-2008, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   03-AUG-2022, entry version 64.
DE   RecName: Full=Seed biotin-containing protein SBP65;
DE   AltName: Full=BP75;
DE   AltName: Full=Seed biotinylated protein of 65 kDa;
GN   Name=SBP65; ORFNames=GmPM4;
OS   Glycine max (Soybean) (Glycine hispida).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC   NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine;
OC   Glycine subgen. Soja.
OX   NCBI_TaxID=3847;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, FUNCTION, AND BIOTINYLATION
RP   AT LYS-125.
RC   STRAIN=cv. Shi-shi; TISSUE=Cotyledon;
RX   PubMed=9747855; DOI=10.1023/a:1006079926339;
RA   Hsing Y.-C., Tsou C.-H., Hsu T.-F., Chen Z.-Y., Hsieh K.-L., Hsieh J.-S.,
RA   Chow T.-Y.;
RT   "Tissue- and stage-specific expression of a soybean (Glycine max L.) seed-
RT   maturation, biotinylated protein.";
RL   Plant Mol. Biol. 38:481-490(1998).
CC   -!- FUNCTION: May serve as a biotin provider for several growth-limiting
CC       enzymes that are necessary during seed development and the subsequent
CC       germination stages, and thus may play some roles in determining seed
CC       germination capacity. {ECO:0000269|PubMed:9747855}.
CC   -!- TISSUE SPECIFICITY: Expressed in the leaf primodium and the vascular
CC       tissues of the hypocotyl-radicle axis of mature seeds. High protein
CC       levels in dry and mature soybean seeds, but not found in fresh immature
CC       seeds. {ECO:0000269|PubMed:9747855}.
CC   -!- SIMILARITY: Belongs to the seed biotin-containing protein SBP65 family.
CC       {ECO:0000305}.
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DR   EMBL; U59626; AAC61783.1; -; mRNA.
DR   PIR; T07064; T07064.
DR   RefSeq; NP_001235522.1; NM_001248593.1.
DR   AlphaFoldDB; Q39846; -.
DR   SMR; Q39846; -.
DR   Allergome; 8270; Gly m 7.
DR   PRIDE; Q39846; -.
DR   GeneID; 547879; -.
DR   KEGG; gmx:547879; -.
DR   eggNOG; ENOG502RYHK; Eukaryota.
DR   InParanoid; Q39846; -.
DR   OrthoDB; 847834at2759; -.
DR   Proteomes; UP000008827; Unplaced.
PE   2: Evidence at transcript level;
KW   Biotin; Coiled coil; Germination; Reference proteome.
FT   CHAIN           1..643
FT                   /note="Seed biotin-containing protein SBP65"
FT                   /id="PRO_0000342636"
FT   REGION          105..262
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          426..480
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          501..522
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COILED          237..279
FT                   /evidence="ECO:0000255"
FT   COMPBIAS        114..182
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        204..220
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        246..262
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        426..457
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOD_RES         125
FT                   /note="N6-biotinyllysine; atypical"
FT                   /evidence="ECO:0000305|PubMed:9747855"
SQ   SEQUENCE   643 AA;  67878 MW;  0D18630D04196985 CRC64;
     MASEQLARRE NTTTEKEIHV EKHRVPKMAT HFEHLAEQAK ESDITAGKDT PQGSIEALQA
     GERVKDHAGK AMGDIGGRGK ARETHELGAH FESLADKVTD HAAANVVGNK ESQREARGGV
     RDVGKFEMRT EGGEKGNKDR PELKTRTREV IGRTEKERGR ESGGQVVAEK GRETETARGR
     VGAENEGART TAVITCTLEK GGGTQKPIRE EERESESERS AWEQISNYSD QATQGVKEKY
     ERAKQAASET LNTTTQTAQE KSAQAKNLAA QAKDATLEKG QQGYAVTKDT ISSAAKTASE
     KTAPVAEKAK DYTLQAAEKA KSAGGTTASY VGEKAVQAKD VAVESGKSAA GYAAKVAADL
     RDKATAVGWA AAHFSAEKTV EGTKAAAHVV EGAAGYAGHK AAELASMSAG AVKGLAASAG
     ETAKEYTAKK KEEAQRELEA KKPSQPQEAE ERPSEGIGET VRQYAQKPKP SEGNPQKEGT
     GSIVFTAIGE TVSSAGEKVK KPFKNTMGGE SEGGGGKEEG KSVIGKSLTS IGEKLGDAKQ
     REELLDNVTG NITEGGGEVL GAVGETVAEI GQNMMKPAEI VQERAHVRQA GGVLDAIGET
     IAEIAETTRV MVSGEDERVL RQSVVLETRV TGRAKHEEGS HGA