ID   SBPI_SARBU              Reviewed;          57 AA.
AC   P26228;
DT   01-MAY-1992, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1992, sequence version 1.
DT   25-MAY-2022, entry version 81.
DE   RecName: Full=Protease inhibitor;
DE   AltName: Full=SBPI;
OS   Sarcophaga bullata (Grey flesh fly) (Neobellieria bullata).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Oestroidea;
OC   Sarcophagidae; Sarcophaga; Neobellieria.
OX   NCBI_TaxID=7385;
RN   [1]
RP   PROTEIN SEQUENCE.
RC   TISSUE=Larval hemolymph;
RX   PubMed=1304909; DOI=10.1002/pro.5560010210;
RA   Papayannopoulos I.A., Biemann K.;
RT   "Amino acid sequence of a protease inhibitor isolated from Sarcophaga
RT   bullata determined by mass spectrometry.";
RL   Protein Sci. 1:278-288(1992).
CC   -!- FUNCTION: Seems to inhibit trypsin.
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DR   PIR; A37294; TIFHBP.
DR   AlphaFoldDB; P26228; -.
DR   SMR; P26228; -.
DR   MEROPS; I02.040; -.
DR   PRIDE; P26228; -.
DR   GO; GO:0004867; F:serine-type endopeptidase inhibitor activity; IEA:UniProtKB-KW.
DR   CDD; cd00109; KU; 1.
DR   Gene3D; 4.10.410.10; -; 1.
DR   InterPro; IPR002223; Kunitz_BPTI.
DR   InterPro; IPR036880; Kunitz_BPTI_sf.
DR   InterPro; IPR020901; Prtase_inh_Kunz-CS.
DR   Pfam; PF00014; Kunitz_BPTI; 1.
DR   PRINTS; PR00759; BASICPTASE.
DR   SMART; SM00131; KU; 1.
DR   SUPFAM; SSF57362; SSF57362; 1.
DR   PROSITE; PS00280; BPTI_KUNITZ_1; 1.
DR   PROSITE; PS50279; BPTI_KUNITZ_2; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Disulfide bond; Protease inhibitor;
KW   Serine protease inhibitor.
FT   CHAIN           1..57
FT                   /note="Protease inhibitor"
FT                   /id="PRO_0000155427"
FT   DOMAIN          6..56
FT                   /note="BPTI/Kunitz inhibitor"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00031"
FT   SITE            16..17
FT                   /note="Reactive bond for trypsin"
FT                   /evidence="ECO:0000250"
FT   DISULFID        6..56
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00031"
FT   DISULFID        15..39
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00031"
FT   DISULFID        31..52
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00031"
SQ   SEQUENCE   57 AA;  6518 MW;  FC512C5399E87241 CRC64;
     VDKSACLQPK EVGPCRKSDF VFFYNADTKA CEEFLYGGCR GNDNRFNTKE ECEKLCL