SBS_SOLHA
ID SBS_SOLHA Reviewed; 777 AA.
AC B8XA41;
DT 08-FEB-2011, integrated into UniProtKB/Swiss-Prot.
DT 03-MAR-2009, sequence version 1.
DT 03-AUG-2022, entry version 46.
DE RecName: Full=Santalene and bergamotene synthase, chloroplastic;
DE AltName: Full=(+)-alpha-santalene synthase ((2Z,6Z)-farnesyl diphosphate cyclizing);
DE EC=4.2.3.50;
DE AltName: Full=(+)-endo-beta-bergamotene synthase ((2Z,6Z)-farnesyl diphosphate cyclizing);
DE EC=4.2.3.53;
DE AltName: Full=(-)-endo-alpha-bergamotene synthase ((2Z,6Z)-farnesyl diphosphate cyclizing);
DE EC=4.2.3.54;
DE Flags: Precursor;
GN Name=SBS;
OS Solanum habrochaites (Wild tomato) (Lycopersicon hirsutum).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC asterids; lamiids; Solanales; Solanaceae; Solanoideae; Solaneae; Solanum;
OC Solanum subgen. Lycopersicon.
OX NCBI_TaxID=62890;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], CATALYTIC ACTIVITY, SUBCELLULAR LOCATION, AND
RP FUNCTION.
RC TISSUE=Trichome gland;
RX PubMed=19155349; DOI=10.1105/tpc.107.057885;
RA Sallaud C., Rontein D., Onillon S., Jabes F., Duffe P., Giacalone C.,
RA Thoraval S., Escoffier C., Herbette G., Leonhardt N., Causse M.,
RA Tissier A.;
RT "A novel pathway for sesquiterpene biosynthesis from Z,Z-farnesyl
RT pyrophosphate in the wild tomato Solanum habrochaites.";
RL Plant Cell 21:301-317(2009).
CC -!- FUNCTION: (2Z,6Z)-farnesyl diphosphate cyclizing enzyme. Produces (+)-
CC alpha-santalene, (+)-endo-beta-bergamotene, (-)-endo-alpha-bergamotene,
CC and at lower amounts, (-)exo-alpha-bergamotene and (+)-epi-beta-
CC santalene. Not able to use geranyl diphosphate, E,E-farnesyl
CC diphosphate or E,E,E-geranylgeranyl diphosphate as substrates, but able
CC to use Neryl diphosphate to make the monoterpene terpineol.
CC {ECO:0000269|PubMed:19155349}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(2Z,6Z)-farnesyl diphosphate = (+)-alpha-santalene +
CC diphosphate; Xref=Rhea:RHEA:30463, ChEBI:CHEBI:33019,
CC ChEBI:CHEBI:60374, ChEBI:CHEBI:61677; EC=4.2.3.50;
CC Evidence={ECO:0000269|PubMed:19155349};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(2Z,6Z)-farnesyl diphosphate = (+)-endo-beta-bergamotene +
CC diphosphate; Xref=Rhea:RHEA:30467, ChEBI:CHEBI:33019,
CC ChEBI:CHEBI:60374, ChEBI:CHEBI:61678; EC=4.2.3.53;
CC Evidence={ECO:0000269|PubMed:19155349};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(2Z,6Z)-farnesyl diphosphate = (1S,5S,6S)-alpha-bergamotene +
CC diphosphate; Xref=Rhea:RHEA:30471, ChEBI:CHEBI:33019,
CC ChEBI:CHEBI:60374, ChEBI:CHEBI:61679; EC=4.2.3.54;
CC Evidence={ECO:0000269|PubMed:19155349};
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250};
CC Name=Mn(2+); Xref=ChEBI:CHEBI:29035; Evidence={ECO:0000250};
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast
CC {ECO:0000269|PubMed:19155349}.
CC -!- DOMAIN: The Asp-Asp-Xaa-Xaa-Asp/Glu (DDXXD/E) motif is important for
CC the catalytic activity, presumably through binding to Mg(2+).
CC {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the terpene synthase family. Tpse subfamily.
CC {ECO:0000305}.
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DR EMBL; FJ194970; ACJ38409.1; -; mRNA.
DR AlphaFoldDB; B8XA41; -.
DR SMR; B8XA41; -.
DR PRIDE; B8XA41; -.
DR KEGG; ag:ACJ38409; -.
DR BioCyc; MetaCyc:MON-14819; -.
DR BRENDA; 4.2.3.50; 3102.
DR BRENDA; 4.2.3.53; 3102.
DR BRENDA; 4.2.3.54; 3102.
DR GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR GO; GO:0102062; F:alpha-santalene synthase activity; IEA:UniProtKB-EC.
DR GO; GO:0102060; F:endo-alpha-bergamotene synthase activity; IEA:UniProtKB-EC.
DR GO; GO:0102061; F:endo-beta-bergamotene synthase activity; IEA:UniProtKB-EC.
DR GO; GO:0000287; F:magnesium ion binding; IEA:InterPro.
DR GO; GO:0010333; F:terpene synthase activity; IEA:InterPro.
DR Gene3D; 1.10.600.10; -; 1.
DR Gene3D; 1.50.10.130; -; 1.
DR InterPro; IPR008949; Isoprenoid_synthase_dom_sf.
DR InterPro; IPR001906; Terpene_synth_N.
DR InterPro; IPR036965; Terpene_synth_N_sf.
DR InterPro; IPR005630; Terpene_synthase_metal-bd.
DR InterPro; IPR008930; Terpenoid_cyclase/PrenylTrfase.
DR Pfam; PF01397; Terpene_synth; 1.
DR Pfam; PF03936; Terpene_synth_C; 1.
DR SUPFAM; SSF48239; SSF48239; 2.
DR SUPFAM; SSF48576; SSF48576; 1.
PE 1: Evidence at protein level;
KW Chloroplast; Lyase; Magnesium; Manganese; Metal-binding; Plastid;
KW Transit peptide.
FT TRANSIT 1..36
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT CHAIN 37..777
FT /note="Santalene and bergamotene synthase, chloroplastic"
FT /id="PRO_0000405118"
FT MOTIF 530..534
FT /note="DDXXD motif"
FT BINDING 530
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="1"
FT /evidence="ECO:0000250"
FT BINDING 530
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="2"
FT /evidence="ECO:0000250"
FT BINDING 534
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="1"
FT /evidence="ECO:0000250"
FT BINDING 534
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="2"
FT /evidence="ECO:0000250"
SQ SEQUENCE 777 AA; 90626 MW; AE19AD4DA742979D CRC64;
MIVGYRSTII TLSHPKLGNG KTISSNAIFQ RSCRVRCSHS TPSSMNGFED ARDRIRESFG
KVELSPSSYD TAWVAMVPSK HSLNEPCFPQ CLDWIIENQR EDGSWGLNPS HPLLLKDSLS
STLACLLALT KWRVGDEQIK RGLGFIETQS WAIDNKDQIS PLGFEIIFPS MIKSAEKLNL
NLAINKRDST IKRALQNEFT RNIEYMSEGV GELCDWKEII KLHQRQNGSL FDSPATTAAA
LIYHQHDKKC YEYLNSILQQ HKNWVPTMYP TKIHSLLCLV DTLQNLGVHR HFKSEIKKAL
DEIYRLWQQK NEQIFSNVTH CAMAFRLLRM SYYDVSSDEL AEFVDEEHFF AISGKYTSHV
EILELHKASQ LAIDHEKDDI LDKINNWTRT FMEQKLLNNG FIDRMSKKEV ELALRKFYTI
SDLAENRRCI KSYEENNFKI LKAAYRSPNI YNKDLFIFSI RNFELCQAQH QEELQQFKRW
FEDYRLDQLG IAERYIHDTY LCAVIVVPEP ELSDARLLYA KYVLLLTIVD DQFDSFASTD
ECLNIIELVE RWDDYASVGY KSEKVKVFFS TLYKSIEELV TIAEIKQGRS VKNHLLNLWL
ELVKLMLMER VEWFSGKTIP SIEEYLYVTS ITFGARLIPL TTQYFLGIKI SEDILESDEI
YGLCNCTGRV LRILNDLQDS KKEQKEDSVT IVTLLMKSMS EEEAIMKIKE ILEMNRRELL
KMVLVQKKGS QLPQICKDIF WRTSNWADFI YLQTDGYRIA EEMKNHIDEV FYKPLNH
