SBT15_ARATH
ID SBT15_ARATH Reviewed; 775 AA.
AC Q9LUM3; Q8LGA0; Q9C5N5;
DT 20-JAN-2016, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 25-MAY-2022, entry version 146.
DE RecName: Full=Subtilisin-like protease SBT1.5 {ECO:0000303|PubMed:16193095};
DE EC=3.4.21.- {ECO:0000255|PROSITE-ProRule:PRU10082};
DE AltName: Full=Subtilase subfamily 1 member 5 {ECO:0000303|PubMed:16193095};
DE Short=AtSBT1.5 {ECO:0000303|PubMed:16193095};
DE Flags: Precursor;
GN Name=SBT1.5 {ECO:0000303|PubMed:16193095};
GN OrderedLocusNames=At3g14240 {ECO:0000312|Araport:AT3G14240};
GN ORFNames=MLN21.2 {ECO:0000312|EMBL:BAB01030.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10819329; DOI=10.1093/dnares/7.2.131;
RA Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 3. I. Sequence
RT features of the regions of 4,504,864 bp covered by sixty P1 and TAC
RT clones.";
RL DNA Res. 7:131-135(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=16193095; DOI=10.1371/journal.pcbi.0010040;
RA Rautengarten C., Steinhauser D., Bussis D., Stintzi A., Schaller A.,
RA Kopka J., Altmann T.;
RT "Inferring hypotheses on functional relationships of genes: Analysis of the
RT Arabidopsis thaliana subtilase gene family.";
RL PLoS Comput. Biol. 1:E40-E40(2005).
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250|UniProtKB:Q84WS0}.
CC -!- SIMILARITY: Belongs to the peptidase S8 family. {ECO:0000305}.
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DR EMBL; AB022220; BAB01030.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE75493.1; -; Genomic_DNA.
DR EMBL; AF360129; AAK25839.1; -; mRNA.
DR EMBL; AY084387; AAM60964.1; -; mRNA.
DR RefSeq; NP_566483.1; NM_112282.3.
DR AlphaFoldDB; Q9LUM3; -.
DR SMR; Q9LUM3; -.
DR STRING; 3702.AT3G14240.1; -.
DR MEROPS; S08.A44; -.
DR PaxDb; Q9LUM3; -.
DR PRIDE; Q9LUM3; -.
DR ProteomicsDB; 232844; -.
DR EnsemblPlants; AT3G14240.1; AT3G14240.1; AT3G14240.
DR GeneID; 820644; -.
DR Gramene; AT3G14240.1; AT3G14240.1; AT3G14240.
DR KEGG; ath:AT3G14240; -.
DR Araport; AT3G14240; -.
DR TAIR; locus:2091010; AT3G14240.
DR eggNOG; ENOG502QTK5; Eukaryota.
DR HOGENOM; CLU_000625_3_1_1; -.
DR InParanoid; Q9LUM3; -.
DR OMA; YSASMCL; -.
DR OrthoDB; 337164at2759; -.
DR PhylomeDB; Q9LUM3; -.
DR PRO; PR:Q9LUM3; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q9LUM3; baseline and differential.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0016020; C:membrane; IBA:GO_Central.
DR GO; GO:0004252; F:serine-type endopeptidase activity; IBA:GO_Central.
DR GO; GO:0016485; P:protein processing; IBA:GO_Central.
DR CDD; cd04852; Peptidases_S8_3; 1.
DR Gene3D; 3.30.70.80; -; 1.
DR Gene3D; 3.40.50.200; -; 1.
DR InterPro; IPR003137; PA_domain.
DR InterPro; IPR000209; Peptidase_S8/S53_dom.
DR InterPro; IPR036852; Peptidase_S8/S53_dom_sf.
DR InterPro; IPR023828; Peptidase_S8_Ser-AS.
DR InterPro; IPR015500; Peptidase_S8_subtilisin-rel.
DR InterPro; IPR034197; Peptidases_S8_3.
DR InterPro; IPR010259; S8pro/Inhibitor_I9.
DR InterPro; IPR037045; S8pro/Inhibitor_I9_sf.
DR InterPro; IPR041469; Subtilisin-like_FN3.
DR Pfam; PF17766; fn3_6; 1.
DR Pfam; PF05922; Inhibitor_I9; 1.
DR Pfam; PF02225; PA; 1.
DR Pfam; PF00082; Peptidase_S8; 1.
DR PRINTS; PR00723; SUBTILISIN.
DR SUPFAM; SSF52743; SSF52743; 1.
DR PROSITE; PS51892; SUBTILASE; 1.
DR PROSITE; PS00138; SUBTILASE_SER; 1.
PE 2: Evidence at transcript level;
KW Autocatalytic cleavage; Glycoprotein; Hydrolase; Protease;
KW Reference proteome; Secreted; Serine protease; Signal; Zymogen.
FT SIGNAL 1..19
FT /evidence="ECO:0000255"
FT PROPEP 20..103
FT /note="Activation peptide"
FT /evidence="ECO:0000250|UniProtKB:Q9MAP7"
FT /id="PRO_0000435174"
FT CHAIN 104..?
FT /note="Subtilisin-like protease SBT1.5"
FT /id="PRO_5004330100"
FT PROPEP ?..775
FT /evidence="ECO:0000250|UniProtKB:Q39547"
FT /id="PRO_0000435175"
FT DOMAIN 27..103
FT /note="Inhibitor I9"
FT /evidence="ECO:0000255"
FT DOMAIN 107..617
FT /note="Peptidase S8"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01240"
FT DOMAIN 367..459
FT /note="PA"
FT /evidence="ECO:0000255"
FT ACT_SITE 137
FT /note="Charge relay system"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01240"
FT ACT_SITE 210
FT /note="Charge relay system"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01240"
FT ACT_SITE 549
FT /note="Charge relay system"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01240"
FT CARBOHYD 196
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 599
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 638
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 762
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CONFLICT 5
FT /note="F -> S (in Ref. 3; AAK25839)"
FT /evidence="ECO:0000305"
FT CONFLICT 45
FT /note="F -> L (in Ref. 4; AAM60964)"
FT /evidence="ECO:0000305"
FT CONFLICT 67
FT /note="D -> N (in Ref. 4; AAM60964)"
FT /evidence="ECO:0000305"
FT CONFLICT 398
FT /note="K -> T (in Ref. 4; AAM60964)"
FT /evidence="ECO:0000305"
FT CONFLICT 579
FT /note="I -> M (in Ref. 4; AAM60964)"
FT /evidence="ECO:0000305"
FT CONFLICT 604
FT /note="M -> T (in Ref. 4; AAM60964)"
FT /evidence="ECO:0000305"
FT CONFLICT 615
FT /note="K -> R (in Ref. 4; AAM60964)"
FT /evidence="ECO:0000305"
FT CONFLICT 641
FT /note="R -> G (in Ref. 4; AAM60964)"
FT /evidence="ECO:0000305"
FT CONFLICT 754
FT /note="I -> M (in Ref. 4; AAM60964)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 775 AA; 82584 MW; CC909DE4DABDE0E8 CRC64;
MAFFFYFFFL LTLSSPSSSA SSSNSLTYIV HVDHEAKPSI FPTHFHWYTS SLASLTSSPP
SIIHTYDTVF HGFSARLTSQ DASQLLDHPH VISVIPEQVR HLHTTRSPEF LGLRSTDKAG
LLEESDFGSD LVIGVIDTGV WPERPSFDDR GLGPVPIKWK GQCIASQDFP ESACNRKLVG
ARFFCGGYEA TNGKMNETTE FRSPRDSDGH GTHTASISAG RYVFPASTLG YAHGVAAGMA
PKARLAAYKV CWNSGCYDSD ILAAFDTAVA DGVDVISLSV GGVVVPYYLD AIAIGAFGAI
DRGIFVSASA GNGGPGALTV TNVAPWMTTV GAGTIDRDFP ANVKLGNGKM ISGVSVYGGP
GLDPGRMYPL VYGGSLLGGD GYSSSLCLEG SLDPNLVKGK IVLCDRGINS RATKGEIVRK
NGGLGMIIAN GVFDGEGLVA DCHVLPATSV GASGGDEIRR YISESSKSRS SKHPTATIVF
KGTRLGIRPA PVVASFSARG PNPETPEILK PDVIAPGLNI LAAWPDRIGP SGVTSDNRRT
EFNILSGTSM ACPHVSGLAA LLKAAHPDWS PAAIRSALIT TAYTVDNSGE PMMDESTGNT
SSVMDYGSGH VHPTKAMDPG LVYDITSYDY INFLCNSNYT RTNIVTITRR QADCDGARRA
GHVGNLNYPS FSVVFQQYGE SKMSTHFIRT VTNVGDSDSV YEIKIRPPRG TTVTVEPEKL
SFRRVGQKLS FVVRVKTTEV KLSPGATNVE TGHIVWSDGK RNVTSPLVVT LQQPL
