SBT16_ARATH
ID SBT16_ARATH Reviewed; 764 AA.
AC O49607;
DT 04-MAR-2015, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-1998, sequence version 1.
DT 03-AUG-2022, entry version 154.
DE RecName: Full=Subtilisin-like protease SBT1.6 {ECO:0000303|PubMed:16193095};
DE EC=3.4.21.- {ECO:0000305};
DE AltName: Full=Subtilase subfamily 1 member 6 {ECO:0000303|PubMed:16193095};
DE Short=AtSBT1.6 {ECO:0000303|PubMed:16193095};
DE AltName: Full=Subtilisin-like serine protease 2 {ECO:0000303|PubMed:12702015};
DE Short=At-SLP2 {ECO:0000303|PubMed:12702015};
DE Flags: Precursor;
GN Name=SBT1.6 {ECO:0000303|PubMed:16193095};
GN Synonyms=SLP2 {ECO:0000303|PubMed:12702015};
GN OrderedLocusNames=At4g34980 {ECO:0000312|Araport:AT4G34980};
GN ORFNames=M4E13.40 {ECO:0000312|EMBL:CAA17763.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617198; DOI=10.1038/47134;
RA Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T.,
RA Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B.,
RA Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M.,
RA de Simone V., Obermaier B., Mache R., Mueller M., Kreis M., Delseny M.,
RA Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D.,
RA Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J.,
RA Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B.,
RA Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J.,
RA Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R.,
RA Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M.,
RA Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P.,
RA Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S.,
RA Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C.,
RA Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J.,
RA Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S.,
RA Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A.,
RA Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M.,
RA Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D.,
RA Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E.,
RA Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S.,
RA Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R.,
RA Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M.,
RA Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E.,
RA Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P.,
RA Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K.,
RA Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K.,
RA de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K.,
RA Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M.,
RA Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G.,
RA Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K.,
RA Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K.,
RA Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W.,
RA Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H.,
RA Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B.,
RA Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J.,
RA Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K.,
RA O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N.,
RA Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A.,
RA Martienssen R., McCombie W.R.;
RT "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.";
RL Nature 402:769-777(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP TISSUE SPECIFICITY, AND INDUCTION BY METHYL JASMONATE.
RX PubMed=12702015; DOI=10.1034/j.1399-3054.2003.00087.x;
RA Golldack D., Vera P., Dietz K.J.;
RT "Expression of subtilisin-like serine proteases in Arabidopsis thaliana is
RT cell-specific and responds to jasmonic acid and heavy metals with
RT developmental differences.";
RL Physiol. Plantarum 118:64-73(2003).
RN [5]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=16193095; DOI=10.1371/journal.pcbi.0010040;
RA Rautengarten C., Steinhauser D., Bussis D., Stintzi A., Schaller A.,
RA Kopka J., Altmann T.;
RT "Inferring hypotheses on functional relationships of genes: Analysis of the
RT Arabidopsis thaliana subtilase gene family.";
RL PLoS Comput. Biol. 1:E40-E40(2005).
CC -!- TISSUE SPECIFICITY: Expressed in roots, leaves and flowers of mature
CC plants. {ECO:0000269|PubMed:12702015}.
CC -!- INDUCTION: By methyl jasmonate. {ECO:0000269|PubMed:12702015}.
CC -!- SIMILARITY: Belongs to the peptidase S8 family. {ECO:0000305}.
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DR EMBL; AL022023; CAA17763.1; -; Genomic_DNA.
DR EMBL; AL161586; CAB80215.1; -; Genomic_DNA.
DR EMBL; CP002687; AEE86443.1; -; Genomic_DNA.
DR EMBL; AY074375; AAL67071.1; -; mRNA.
DR EMBL; AY096357; AAM19998.1; -; mRNA.
DR PIR; T05768; T05768.
DR RefSeq; NP_567972.1; NM_119664.3.
DR AlphaFoldDB; O49607; -.
DR SMR; O49607; -.
DR STRING; 3702.AT4G34980.1; -.
DR MEROPS; S08.A39; -.
DR PaxDb; O49607; -.
DR PRIDE; O49607; -.
DR ProMEX; O49607; -.
DR ProteomicsDB; 232957; -.
DR EnsemblPlants; AT4G34980.1; AT4G34980.1; AT4G34980.
DR GeneID; 829650; -.
DR Gramene; AT4G34980.1; AT4G34980.1; AT4G34980.
DR KEGG; ath:AT4G34980; -.
DR Araport; AT4G34980; -.
DR TAIR; locus:2131566; AT4G34980.
DR eggNOG; ENOG502QPQR; Eukaryota.
DR HOGENOM; CLU_000625_4_6_1; -.
DR InParanoid; O49607; -.
DR OMA; GRMFPVV; -.
DR OrthoDB; 337164at2759; -.
DR PhylomeDB; O49607; -.
DR PRO; PR:O49607; -.
DR Proteomes; UP000006548; Chromosome 4.
DR ExpressionAtlas; O49607; baseline and differential.
DR Genevisible; O49607; AT.
DR GO; GO:0005886; C:plasma membrane; HDA:TAIR.
DR GO; GO:0004252; F:serine-type endopeptidase activity; IEA:InterPro.
DR GO; GO:0008236; F:serine-type peptidase activity; ISS:TAIR.
DR GO; GO:0009827; P:plant-type cell wall modification; TAS:TAIR.
DR GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR CDD; cd04852; Peptidases_S8_3; 1.
DR Gene3D; 3.30.70.80; -; 1.
DR Gene3D; 3.40.50.200; -; 1.
DR InterPro; IPR003137; PA_domain.
DR InterPro; IPR000209; Peptidase_S8/S53_dom.
DR InterPro; IPR036852; Peptidase_S8/S53_dom_sf.
DR InterPro; IPR023828; Peptidase_S8_Ser-AS.
DR InterPro; IPR015500; Peptidase_S8_subtilisin-rel.
DR InterPro; IPR034197; Peptidases_S8_3.
DR InterPro; IPR010259; S8pro/Inhibitor_I9.
DR InterPro; IPR037045; S8pro/Inhibitor_I9_sf.
DR InterPro; IPR045051; SBT.
DR InterPro; IPR041469; Subtilisin-like_FN3.
DR PANTHER; PTHR10795; PTHR10795; 1.
DR Pfam; PF17766; fn3_6; 1.
DR Pfam; PF05922; Inhibitor_I9; 1.
DR Pfam; PF02225; PA; 1.
DR Pfam; PF00082; Peptidase_S8; 1.
DR PRINTS; PR00723; SUBTILISIN.
DR SUPFAM; SSF52743; SSF52743; 1.
DR PROSITE; PS51892; SUBTILASE; 1.
DR PROSITE; PS00138; SUBTILASE_SER; 1.
PE 2: Evidence at transcript level;
KW Glycoprotein; Hydrolase; Protease; Reference proteome; Serine protease;
KW Signal.
FT SIGNAL 1..20
FT /evidence="ECO:0000255"
FT CHAIN 21..764
FT /note="Subtilisin-like protease SBT1.6"
FT /evidence="ECO:0000255"
FT /id="PRO_0000431965"
FT DOMAIN 46..99
FT /note="Inhibitor I9"
FT /evidence="ECO:0000255"
FT DOMAIN 103..606
FT /note="Peptidase S8"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01240"
FT DOMAIN 377..457
FT /note="PA"
FT /evidence="ECO:0000255"
FT ACT_SITE 131
FT /note="Charge relay system"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01240"
FT ACT_SITE 205
FT /note="Charge relay system"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01240"
FT ACT_SITE 538
FT /note="Charge relay system"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01240"
FT CARBOHYD 191
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 578
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
SQ SEQUENCE 764 AA; 81046 MW; EBB3827F26F33F50 CRC64;
MASSTIVLLL FLSFPFISFA ASQAAKTFIF RIDGGSMPSI FPTHYHWYST EFAEESRIVH
VYHTVFHGFS AVVTPDEADN LRNHPAVLAV FEDRRRELHT TRSPQFLGLQ NQKGLWSESD
YGSDVIIGVF DTGIWPERRS FSDLNLGPIP KRWRGVCESG ARFSPRNCNR KIIGARFFAK
GQQAAVIGGI NKTVEFLSPR DADGHGTHTS STAAGRHAFK ASMSGYASGV AKGVAPKARI
AAYKVCWKDS GCLDSDILAA FDAAVRDGVD VISISIGGGD GITSPYYLDP IAIGSYGAAS
KGIFVSSSAG NEGPNGMSVT NLAPWVTTVG ASTIDRNFPA DAILGDGHRL RGVSLYAGVP
LNGRMFPVVY PGKSGMSSAS LCMENTLDPK QVRGKIVICD RGSSPRVAKG LVVKKAGGVG
MILANGASNG EGLVGDAHLI PACAVGSNEG DRIKAYASSH PNPIASIDFR GTIVGIKPAP
VIASFSGRGP NGLSPEILKP DLIAPGVNIL AAWTDAVGPT GLPSDPRKTE FNILSGTSMA
CPHVSGAAAL LKSAHPDWSP AVIRSAMMTT TNLVDNSNRS LIDESTGKSA TPYDYGSGHL
NLGRAMNPGL VYDITNDDYI TFLCSIGYGP KTIQVITRTP VRCPTTRKPS PGNLNYPSIT
AVFPTNRRGL VSKTVIRTAT NVGQAEAVYR ARIESPRGVT VTVKPPRLVF TSAVKRRSYA
VTVTVNTRNV VLGETGAVFG SVTWFDGGKH VVRSPIVVTQ MDTL
