SBT22_ARATH
ID SBT22_ARATH Reviewed; 856 AA.
AC Q9SUN6; F4JVJ6;
DT 20-JAN-2016, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 160.
DE RecName: Full=Subtilisin-like protease SBT2.2 {ECO:0000303|PubMed:16193095};
DE EC=3.4.21.- {ECO:0000255|PROSITE-ProRule:PRU10082};
DE AltName: Full=Subtilase subfamily 2 member 2 {ECO:0000303|PubMed:16193095};
DE Short=AtSBT2.2 {ECO:0000303|PubMed:16193095};
DE Flags: Precursor;
GN Name=SBT2.2 {ECO:0000303|PubMed:16193095};
GN OrderedLocusNames=At4g20430 {ECO:0000312|Araport:AT4G20430};
GN ORFNames=F9F13.80 {ECO:0000312|EMBL:CAB45809.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617198; DOI=10.1038/47134;
RA Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T.,
RA Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B.,
RA Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M.,
RA de Simone V., Obermaier B., Mache R., Mueller M., Kreis M., Delseny M.,
RA Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D.,
RA Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J.,
RA Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B.,
RA Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J.,
RA Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R.,
RA Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M.,
RA Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P.,
RA Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S.,
RA Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C.,
RA Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J.,
RA Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S.,
RA Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A.,
RA Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M.,
RA Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D.,
RA Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E.,
RA Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S.,
RA Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R.,
RA Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M.,
RA Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E.,
RA Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P.,
RA Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K.,
RA Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K.,
RA de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K.,
RA Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M.,
RA Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G.,
RA Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K.,
RA Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K.,
RA Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W.,
RA Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H.,
RA Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B.,
RA Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J.,
RA Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K.,
RA O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N.,
RA Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A.,
RA Martienssen R., McCombie W.R.;
RT "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.";
RL Nature 402:769-777(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=16193095; DOI=10.1371/journal.pcbi.0010040;
RA Rautengarten C., Steinhauser D., Bussis D., Stintzi A., Schaller A.,
RA Kopka J., Altmann T.;
RT "Inferring hypotheses on functional relationships of genes: Analysis of the
RT Arabidopsis thaliana subtilase gene family.";
RL PLoS Comput. Biol. 1:E40-E40(2005).
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250|UniProtKB:Q84WS0}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q9SUN6-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q9SUN6-2; Sequence=VSP_058024;
CC -!- SIMILARITY: Belongs to the peptidase S8 family. {ECO:0000305}.
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DR EMBL; AL080253; CAB45809.1; -; Genomic_DNA.
DR EMBL; AL161553; CAB79043.1; -; Genomic_DNA.
DR EMBL; CP002687; AEE84328.1; -; Genomic_DNA.
DR EMBL; CP002687; AEE84329.1; -; Genomic_DNA.
DR EMBL; CP002687; ANM66822.1; -; Genomic_DNA.
DR PIR; T10585; T10585.
DR RefSeq; NP_001190781.1; NM_001203852.1. [Q9SUN6-2]
DR RefSeq; NP_001320010.1; NM_001341438.1. [Q9SUN6-1]
DR RefSeq; NP_567601.1; NM_118162.3. [Q9SUN6-1]
DR AlphaFoldDB; Q9SUN6; -.
DR SMR; Q9SUN6; -.
DR STRING; 3702.AT4G20430.1; -.
DR MEROPS; S08.A01; -.
DR PaxDb; Q9SUN6; -.
DR PRIDE; Q9SUN6; -.
DR ProteomicsDB; 232931; -. [Q9SUN6-1]
DR EnsemblPlants; AT4G20430.1; AT4G20430.1; AT4G20430. [Q9SUN6-1]
DR EnsemblPlants; AT4G20430.2; AT4G20430.2; AT4G20430. [Q9SUN6-2]
DR EnsemblPlants; AT4G20430.3; AT4G20430.3; AT4G20430. [Q9SUN6-1]
DR GeneID; 827791; -.
DR Gramene; AT4G20430.1; AT4G20430.1; AT4G20430. [Q9SUN6-1]
DR Gramene; AT4G20430.2; AT4G20430.2; AT4G20430. [Q9SUN6-2]
DR Gramene; AT4G20430.3; AT4G20430.3; AT4G20430. [Q9SUN6-1]
DR KEGG; ath:AT4G20430; -.
DR Araport; AT4G20430; -.
DR TAIR; locus:2128595; AT4G20430.
DR eggNOG; ENOG502QSVR; Eukaryota.
DR InParanoid; Q9SUN6; -.
DR OMA; KSRYWRS; -.
DR OrthoDB; 337164at2759; -.
DR PhylomeDB; Q9SUN6; -.
DR PRO; PR:Q9SUN6; -.
DR Proteomes; UP000006548; Chromosome 4.
DR ExpressionAtlas; Q9SUN6; baseline and differential.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0004252; F:serine-type endopeptidase activity; IEA:InterPro.
DR GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR CDD; cd04852; Peptidases_S8_3; 1.
DR Gene3D; 3.40.50.200; -; 1.
DR InterPro; IPR000209; Peptidase_S8/S53_dom.
DR InterPro; IPR036852; Peptidase_S8/S53_dom_sf.
DR InterPro; IPR023827; Peptidase_S8_Asp-AS.
DR InterPro; IPR023828; Peptidase_S8_Ser-AS.
DR InterPro; IPR015500; Peptidase_S8_subtilisin-rel.
DR InterPro; IPR034197; Peptidases_S8_3.
DR InterPro; IPR010259; S8pro/Inhibitor_I9.
DR InterPro; IPR045051; SBT.
DR InterPro; IPR041469; Subtilisin-like_FN3.
DR PANTHER; PTHR10795; PTHR10795; 1.
DR Pfam; PF17766; fn3_6; 1.
DR Pfam; PF05922; Inhibitor_I9; 1.
DR Pfam; PF00082; Peptidase_S8; 1.
DR PRINTS; PR00723; SUBTILISIN.
DR SUPFAM; SSF52743; SSF52743; 1.
DR PROSITE; PS51892; SUBTILASE; 1.
DR PROSITE; PS00136; SUBTILASE_ASP; 1.
DR PROSITE; PS00138; SUBTILASE_SER; 1.
PE 3: Inferred from homology;
KW Alternative splicing; Autocatalytic cleavage; Glycoprotein; Hydrolase;
KW Protease; Reference proteome; Secreted; Serine protease; Signal; Zymogen.
FT SIGNAL 1..21
FT /evidence="ECO:0000255"
FT PROPEP 22..159
FT /note="Activation peptide"
FT /evidence="ECO:0000250|UniProtKB:Q9MAP7"
FT /id="PRO_0000435182"
FT CHAIN 160..?
FT /note="Subtilisin-like protease SBT2.2"
FT /id="PRO_5004337287"
FT PROPEP ?..856
FT /evidence="ECO:0000250|UniProtKB:Q39547"
FT /id="PRO_0000435183"
FT DOMAIN 40..159
FT /note="Inhibitor I9"
FT /evidence="ECO:0000255"
FT DOMAIN 164..709
FT /note="Peptidase S8"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01240"
FT DOMAIN 432..528
FT /note="PA"
FT /evidence="ECO:0000255"
FT ACT_SITE 193
FT /note="Charge relay system"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01240"
FT ACT_SITE 269
FT /note="Charge relay system"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01240"
FT ACT_SITE 634
FT /note="Charge relay system"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01240"
FT CARBOHYD 35
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 85
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 204
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 255
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 412
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 441
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 495
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 540
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 568
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 704
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 730
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 738
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 748
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 767
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 782
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 823
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT VAR_SEQ 450..474
FT /note="MYVGECQDYGSFDKDVIRGNLLICS -> I (in isoform 2)"
FT /id="VSP_058024"
SQ SEQUENCE 856 AA; 91815 MW; 0C9FD624F5DF81E2 CRC64;
MRRVLMVNFG VLLLFCFGVL SNSFGQDNGG DSDINSTTAV YIVTLRQASS LHLFQQEAEE
VKRVRDQSKH GDTSKFTRPK LQPRNISRSR YWRSRRSAIA QAHDSLLRNA LKGEKYIKLY
SFHYLINGFA VFVSSQQAET LSRRREVANI VLDFSVRTAT TYTPQFMGLP KGAWVKEGGY
ETAGEGIVIG FIDTGIDPTH PSFNGTDTSQ RQYPIPNHFS GVCEVTPDFP SGSCNRKLVG
ARHFAQSAIT RGIFNSSEDY ASPFDGDGHG THTASIAAGN HGVSAVVSGH NFGSASGIAP
RAHISVYKAL YKSFGGFAAD VVAAIDQAAQ DGVDILSLSI TPNRRPPGVA TFFNPLDMAM
LSAVKAGIFV VQAAGNTGPS PKSMSSFSPW IFTVGAASHD RDYSNSIVLG NNVSIPGVGL
ALRTDEGKKY TMISALDALK NKSSVVDKDM YVGECQDYGS FDKDVIRGNL LICSYSIRFV
LGLSTIKQAL AVAKNLSAKG VVFYMDPYVL GFQINPTPMD MPGIIIPSAE DSKVLLKYYN
SSLVRDGTTK EIVRFGAVAA IAGGQNANFS NRAPKIMYYS ARGPDPQDSL FNDADILKPN
LVAPGNSIWG AWSSAATEST EFEGESFAMM SGTSMAAPHV AGVAALVKQK FRKFSPSAIA
SALSTTSVLF DNKGEAIMAQ RAYANPDQTI SPATPFDMGN GFVNATAALD PGLIFDTSFE
DYMSFLCGIN GSAPVVFNYT GTNCLRNNAT ISGSDLNLPS ITVSKLNNTR TVQRLMTNIA
GNETYTVSLI TPFDVLINVS PTQFSIASGE TKLLSVILTA KRNSSISSFG GIKLLGNAGH
IVRIPVSVTV KIASKQ
