SBT23_ARATH
ID SBT23_ARATH Reviewed; 840 AA.
AC Q9FI12;
DT 20-JAN-2016, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 03-AUG-2022, entry version 150.
DE RecName: Full=Subtilisin-like protease SBT2.3 {ECO:0000303|PubMed:16193095};
DE EC=3.4.21.- {ECO:0000255|PROSITE-ProRule:PRU10082};
DE AltName: Full=Subtilase subfamily 2 member 3 {ECO:0000303|PubMed:16193095};
DE Short=AtSBT2.3 {ECO:0000303|PubMed:16193095};
DE Flags: Precursor;
GN Name=SBT2.3 {ECO:0000303|PubMed:16193095};
GN OrderedLocusNames=At5g44530 {ECO:0000312|Araport:AT5G44530};
GN ORFNames=MFC16.21 {ECO:0000312|EMBL:BAB09160.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10470850; DOI=10.1093/dnares/6.3.183;
RA Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Kotani H.,
RA Miyajima N., Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. IX. Sequence
RT features of the regions of 1,011,550 bp covered by seventeen P1 and TAC
RT clones.";
RL DNA Res. 6:183-195(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Cheuk R.F., Chen H., Kim C.J., Shinn P., Carninci P., Hayashizaki Y.,
RA Ishida J., Kamiya A., Kawai J., Narusaka M., Sakurai T., Satou M., Seki M.,
RA Shinozaki K., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=16193095; DOI=10.1371/journal.pcbi.0010040;
RA Rautengarten C., Steinhauser D., Bussis D., Stintzi A., Schaller A.,
RA Kopka J., Altmann T.;
RT "Inferring hypotheses on functional relationships of genes: Analysis of the
RT Arabidopsis thaliana subtilase gene family.";
RL PLoS Comput. Biol. 1:E40-E40(2005).
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250|UniProtKB:Q84WS0}.
CC -!- SIMILARITY: Belongs to the peptidase S8 family. {ECO:0000305}.
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DR EMBL; AB017065; BAB09160.1; -; Genomic_DNA.
DR EMBL; CP002688; AED95120.1; -; Genomic_DNA.
DR EMBL; BT014856; AAT41839.1; -; mRNA.
DR EMBL; AK230467; BAF02261.1; -; mRNA.
DR RefSeq; NP_568634.1; NM_123820.3.
DR AlphaFoldDB; Q9FI12; -.
DR SMR; Q9FI12; -.
DR STRING; 3702.AT5G44530.1; -.
DR MEROPS; S08.A04; -.
DR iPTMnet; Q9FI12; -.
DR PaxDb; Q9FI12; -.
DR PRIDE; Q9FI12; -.
DR EnsemblPlants; AT5G44530.1; AT5G44530.1; AT5G44530.
DR GeneID; 834480; -.
DR Gramene; AT5G44530.1; AT5G44530.1; AT5G44530.
DR KEGG; ath:AT5G44530; -.
DR Araport; AT5G44530; -.
DR TAIR; locus:2163446; AT5G44530.
DR eggNOG; ENOG502QSVR; Eukaryota.
DR HOGENOM; CLU_000625_3_1_1; -.
DR InParanoid; Q9FI12; -.
DR OMA; NDNKGGP; -.
DR PhylomeDB; Q9FI12; -.
DR PRO; PR:Q9FI12; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q9FI12; baseline and differential.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0004252; F:serine-type endopeptidase activity; IEA:InterPro.
DR GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR CDD; cd04852; Peptidases_S8_3; 1.
DR Gene3D; 3.30.70.80; -; 1.
DR Gene3D; 3.40.50.200; -; 1.
DR InterPro; IPR000209; Peptidase_S8/S53_dom.
DR InterPro; IPR036852; Peptidase_S8/S53_dom_sf.
DR InterPro; IPR023827; Peptidase_S8_Asp-AS.
DR InterPro; IPR023828; Peptidase_S8_Ser-AS.
DR InterPro; IPR015500; Peptidase_S8_subtilisin-rel.
DR InterPro; IPR034197; Peptidases_S8_3.
DR InterPro; IPR010259; S8pro/Inhibitor_I9.
DR InterPro; IPR037045; S8pro/Inhibitor_I9_sf.
DR InterPro; IPR045051; SBT.
DR InterPro; IPR041469; Subtilisin-like_FN3.
DR PANTHER; PTHR10795; PTHR10795; 1.
DR Pfam; PF17766; fn3_6; 1.
DR Pfam; PF05922; Inhibitor_I9; 1.
DR Pfam; PF00082; Peptidase_S8; 1.
DR PRINTS; PR00723; SUBTILISIN.
DR SUPFAM; SSF52743; SSF52743; 1.
DR PROSITE; PS51892; SUBTILASE; 1.
DR PROSITE; PS00136; SUBTILASE_ASP; 1.
DR PROSITE; PS00138; SUBTILASE_SER; 1.
PE 2: Evidence at transcript level;
KW Autocatalytic cleavage; Glycoprotein; Hydrolase; Protease;
KW Reference proteome; Secreted; Serine protease; Signal; Zymogen.
FT SIGNAL 1..27
FT /evidence="ECO:0000255"
FT PROPEP 28..146
FT /note="Activation peptide"
FT /evidence="ECO:0000250|UniProtKB:Q9MAP7"
FT /id="PRO_0000435184"
FT CHAIN 147..?
FT /note="Subtilisin-like protease SBT2.3"
FT /id="PRO_5004326410"
FT PROPEP ?..840
FT /evidence="ECO:0000250|UniProtKB:Q39547"
FT /id="PRO_0000435185"
FT DOMAIN 38..146
FT /note="Inhibitor I9"
FT /evidence="ECO:0000255"
FT DOMAIN 148..694
FT /note="Peptidase S8"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01240"
FT DOMAIN 418..513
FT /note="PA"
FT /evidence="ECO:0000255"
FT REGION 61..85
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 62..83
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 180
FT /note="Charge relay system"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01240"
FT ACT_SITE 255
FT /note="Charge relay system"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01240"
FT ACT_SITE 619
FT /note="Charge relay system"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01240"
FT CARBOHYD 72
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 193
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 241
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 398
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 427
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 480
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 525
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 553
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 689
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 715
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 723
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 767
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 808
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
SQ SEQUENCE 840 AA; 90617 MW; 0A97E5641FBDEF84 CRC64;
MVRVMLVRFG FLLLMISFVF LSNNTLGQQQ DDDDDSAVYI VTLKQPPIVH LFEEQELKHK
KSKFTPKLRP RNNSRKRHGK SKIPSVVQSH DSFLRKTLKG EKYIKLYSYH YLINGFALFI
NSQQAEKLSM RKEVANIVLD YSVRTATTYT PQFMGLPQGA WVKEGGFEIA GEGVIIGFID
TGIDPNHPSF NDNDSKRSYP IPKHFSGVCE VTPDFPSGSC NKKLIGARHF AQSAVTRGIF
NSSEDYASPF DGDGHGTHTA SVAAGNHGVP VIVSNHNFGY ASGIAPRAFI SVYKALYKSF
GGFAADVVAA IDQAAQDGVD ILSLSITPNR KPPGVATFFN PIDMALLSAV KAGIFVVQAA
GNTGPAPKTM SSFSPWIFTV GASSHDRVYS NSLTLGNNVT IPGMGFAIPT DSGKMYKMIS
AFHALNNSTS VDKDMYVGEC QDYENFDQDR VSGKLLICSY SARFVLGLST IKQALDVAKN
LSATGVIFYI DPYVLGFEIN PTPMDMPGII IPSVEDSKTL LKYYNSSIQR DVTTKEIVSF
GAVAAIEGGL NANFSNRAPK VMYYSARGPD PEDNSFNDAD VLKPNLVAPG NSIWGAWSSA
STDSTEFEGE KFAMMSGTSM AAPHVAGVAA LIKQSYPQFT PSTISSALST TALLNDNKGS
PIMAQRTYSN PDQSLYTATP SDMGSGFVNA TAALDPGLVF DTSFEDYISF LCGINGSDTV
VFNYTGFRCP ANNTPVSGFD LNLPSITVST LSGTQTFQRS MRNIAGNETY NVGWSPPYGV
SMKVSPTQFS IAMGENQVLS VTLTVTKNSS SSSFGRIGLF GNTGHIVNIP VTVIAKIASS
