SBT32_ARATH
ID SBT32_ARATH Reviewed; 734 AA.
AC Q9MAP4; Q682P0;
DT 20-JAN-2016, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 148.
DE RecName: Full=Subtilisin-like protease SBT3.2 {ECO:0000303|PubMed:16193095};
DE EC=3.4.21.- {ECO:0000255|PROSITE-ProRule:PRU10082};
DE AltName: Full=Subtilase subfamily 3 member 2 {ECO:0000303|PubMed:16193095};
DE Short=AtSBT3.2 {ECO:0000303|PubMed:16193095};
DE Flags: Precursor;
GN Name=SBT3.2 {ECO:0000303|PubMed:16193095};
GN OrderedLocusNames=At1g32970 {ECO:0000312|Araport:AT1G32970};
GN ORFNames=F9L11.14 {ECO:0000312|EMBL:AAF31279.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (SEP-2004) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=16193095; DOI=10.1371/journal.pcbi.0010040;
RA Rautengarten C., Steinhauser D., Bussis D., Stintzi A., Schaller A.,
RA Kopka J., Altmann T.;
RT "Inferring hypotheses on functional relationships of genes: Analysis of the
RT Arabidopsis thaliana subtilase gene family.";
RL PLoS Comput. Biol. 1:E40-E40(2005).
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250|UniProtKB:Q84WS0}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q9MAP4-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q9MAP4-2; Sequence=VSP_058025, VSP_058026, VSP_058027;
CC -!- SIMILARITY: Belongs to the peptidase S8 family. {ECO:0000305}.
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DR EMBL; AC006424; AAF31279.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE31545.1; -; Genomic_DNA.
DR EMBL; AK175327; BAD43090.1; -; mRNA.
DR PIR; D86454; D86454.
DR RefSeq; NP_174573.1; NM_103030.2. [Q9MAP4-1]
DR AlphaFoldDB; Q9MAP4; -.
DR SMR; Q9MAP4; -.
DR STRING; 3702.AT1G32970.1; -.
DR MEROPS; S08.A41; -.
DR PaxDb; Q9MAP4; -.
DR PRIDE; Q9MAP4; -.
DR EnsemblPlants; AT1G32970.1; AT1G32970.1; AT1G32970. [Q9MAP4-1]
DR GeneID; 840191; -.
DR Gramene; AT1G32970.1; AT1G32970.1; AT1G32970. [Q9MAP4-1]
DR KEGG; ath:AT1G32970; -.
DR Araport; AT1G32970; -.
DR TAIR; locus:2037955; AT1G32970.
DR eggNOG; ENOG502QSF0; Eukaryota.
DR HOGENOM; CLU_000625_4_2_1; -.
DR OMA; MYKACWH; -.
DR OrthoDB; 337164at2759; -.
DR PhylomeDB; Q9MAP4; -.
DR PRO; PR:Q9MAP4; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q9MAP4; baseline and differential.
DR GO; GO:0005615; C:extracellular space; IBA:GO_Central.
DR GO; GO:0004252; F:serine-type endopeptidase activity; IBA:GO_Central.
DR GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR CDD; cd04852; Peptidases_S8_3; 1.
DR Gene3D; 3.30.70.80; -; 1.
DR Gene3D; 3.40.50.200; -; 1.
DR InterPro; IPR000209; Peptidase_S8/S53_dom.
DR InterPro; IPR036852; Peptidase_S8/S53_dom_sf.
DR InterPro; IPR023828; Peptidase_S8_Ser-AS.
DR InterPro; IPR015500; Peptidase_S8_subtilisin-rel.
DR InterPro; IPR034197; Peptidases_S8_3.
DR InterPro; IPR037045; S8pro/Inhibitor_I9_sf.
DR InterPro; IPR041469; Subtilisin-like_FN3.
DR Pfam; PF17766; fn3_6; 1.
DR Pfam; PF00082; Peptidase_S8; 1.
DR PRINTS; PR00723; SUBTILISIN.
DR SUPFAM; SSF52743; SSF52743; 1.
DR PROSITE; PS51892; SUBTILASE; 1.
DR PROSITE; PS00138; SUBTILASE_SER; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Autocatalytic cleavage; Glycoprotein; Hydrolase;
KW Protease; Reference proteome; Secreted; Serine protease; Signal; Zymogen.
FT SIGNAL 1..19
FT /evidence="ECO:0000255"
FT PROPEP 20..88
FT /note="Activation peptide"
FT /evidence="ECO:0000250|UniProtKB:Q39547"
FT /id="PRO_0000435191"
FT CHAIN 89..?
FT /note="Subtilisin-like protease SBT3.2"
FT /evidence="ECO:0000255"
FT /id="PRO_5004329302"
FT PROPEP ?..734
FT /evidence="ECO:0000250|UniProtKB:Q39547"
FT /id="PRO_0000435192"
FT DOMAIN 92..581
FT /note="Peptidase S8"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01240"
FT DOMAIN 361..438
FT /note="PA"
FT /evidence="ECO:0000255"
FT ACT_SITE 122
FT /note="Charge relay system"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01240"
FT ACT_SITE 179
FT /note="Charge relay system"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01240"
FT ACT_SITE 512
FT /note="Charge relay system"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01240"
FT CARBOHYD 108
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 143
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 326
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 355
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 497
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 669
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT VAR_SEQ 1..215
FT /note="Missing (in isoform 2)"
FT /id="VSP_058025"
FT VAR_SEQ 443..453
FT /note="SPVAKIQPTRT -> YITVIHIFIYL (in isoform 2)"
FT /id="VSP_058026"
FT VAR_SEQ 454..734
FT /note="Missing (in isoform 2)"
FT /id="VSP_058027"
SQ SEQUENCE 734 AA; 79164 MW; B519938B9E3ECB28 CRC64;
MTRALILVAI CLMLTLNNAA ETKVHIVYLG EKQHDDPDSV TESHHQMLWS ILGSKEAAHD
SMTPWLLSFR SQTNQFPSES TLRFYELQTT RTWDYLQHTS KHPKNILNQT NMGDQLIIGV
VDSVTLNWFG FILLKQEYGQ SLNHSVTMVL DQYQNVGKEV QLGHAENPEY ISPRDFDGHG
THVAATAAGS FVPDTNYLGL GRGTARGGAP RARIAMYKAC WHLVTGATTC SAADLVKAID
EAIHDGVDVL SISNGFSVPL FPEVDTQDGV AVGAFHAVAK GIPVVCAGGN AGPSSQTISN
TAPWIITVAA TTQDRSFPTF ITLGNNVTVV GQALYQGPDI DFTELVYPED SGASNETFYG
VCEDLAKNPA HIIEEKIVLC FTKSTSYSTM IQAASDVVKL DGYGVIVARN PGHQLSPCFG
FPCLAVDYEL GTDILFYIRS TRSPVAKIQP TRTLVGLPVA TKVATFSSRG PNSISPAILK
PDIAAPGVNI LAATSPNDTF YDKGFAMKSG TSMSAPVVAG IVALLKSVHP HWSPAAIRSA
IVTTAWRTDP SGEPIFADGS NRKLADPFDY GGGVVNSEKA ANPGLVYDMG VKDYILYLCS
VGYTDSSITG LVSKKTVCAN PKPSVLDLNL PSITIPNLAK EVTITRTVTN VGPVGSVYKP
VIEAPMGVNV TVTPSTLVFN AYTRKLSFKV RVLTNHIVNT GYYFGSLTWT DSVHNVVIPV
SVRTQIMQRY YDEN
