SBT3G_ARATH
ID SBT3G_ARATH Reviewed; 759 AA.
AC Q8GWX9; Q9C7U9;
DT 20-JAN-2016, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 03-AUG-2022, entry version 140.
DE RecName: Full=Subtilisin-like protease SBT3.16 {ECO:0000303|PubMed:16193095};
DE EC=3.4.21.- {ECO:0000255|PROSITE-ProRule:PRU10082};
DE AltName: Full=Subtilase subfamily 3 member 16 {ECO:0000303|PubMed:16193095};
DE Short=AtSBT3.16 {ECO:0000303|PubMed:16193095};
DE Flags: Precursor;
GN Name=SBT3.16 {ECO:0000303|PubMed:16193095};
GN OrderedLocusNames=At1g66210 {ECO:0000312|Araport:AT1G66210};
GN ORFNames=T6J19.3 {ECO:0000312|EMBL:AAG51763.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702 {ECO:0000312|EMBL:BAC43166.1};
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=11910074; DOI=10.1126/science.1071006;
RA Seki M., Narusaka M., Kamiya A., Ishida J., Satou M., Sakurai T.,
RA Nakajima M., Enju A., Akiyama K., Oono Y., Muramatsu M., Hayashizaki Y.,
RA Kawai J., Carninci P., Itoh M., Ishii Y., Arakawa T., Shibata K.,
RA Shinagawa A., Shinozaki K.;
RT "Functional annotation of a full-length Arabidopsis cDNA collection.";
RL Science 296:141-145(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=16193095; DOI=10.1371/journal.pcbi.0010040;
RA Rautengarten C., Steinhauser D., Bussis D., Stintzi A., Schaller A.,
RA Kopka J., Altmann T.;
RT "Inferring hypotheses on functional relationships of genes: Analysis of the
RT Arabidopsis thaliana subtilase gene family.";
RL PLoS Comput. Biol. 1:E40-E40(2005).
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250|UniProtKB:Q84WS0}.
CC -!- SIMILARITY: Belongs to the peptidase S8 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAG51763.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AC066691; AAG51763.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE34477.1; -; Genomic_DNA.
DR EMBL; AK118565; BAC43166.1; -; mRNA.
DR EMBL; BT005956; AAO64891.1; -; mRNA.
DR PIR; A96687; A96687.
DR RefSeq; NP_564868.2; NM_105292.3.
DR AlphaFoldDB; Q8GWX9; -.
DR SMR; Q8GWX9; -.
DR STRING; 3702.AT1G66210.1; -.
DR MEROPS; S08.A34; -.
DR PaxDb; Q8GWX9; -.
DR PRIDE; Q8GWX9; -.
DR ProteomicsDB; 232907; -.
DR EnsemblPlants; AT1G66210.1; AT1G66210.1; AT1G66210.
DR GeneID; 842936; -.
DR Gramene; AT1G66210.1; AT1G66210.1; AT1G66210.
DR KEGG; ath:AT1G66210; -.
DR Araport; AT1G66210; -.
DR TAIR; locus:2205303; AT1G66210.
DR eggNOG; ENOG502QSF0; Eukaryota.
DR HOGENOM; CLU_000625_4_2_1; -.
DR InParanoid; Q8GWX9; -.
DR OMA; KASHESM; -.
DR OrthoDB; 337164at2759; -.
DR PhylomeDB; Q8GWX9; -.
DR PRO; PR:Q8GWX9; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q8GWX9; baseline and differential.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0004252; F:serine-type endopeptidase activity; IEA:InterPro.
DR GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR CDD; cd04852; Peptidases_S8_3; 1.
DR Gene3D; 3.30.70.80; -; 1.
DR Gene3D; 3.40.50.200; -; 1.
DR InterPro; IPR000209; Peptidase_S8/S53_dom.
DR InterPro; IPR036852; Peptidase_S8/S53_dom_sf.
DR InterPro; IPR022398; Peptidase_S8_His-AS.
DR InterPro; IPR023828; Peptidase_S8_Ser-AS.
DR InterPro; IPR015500; Peptidase_S8_subtilisin-rel.
DR InterPro; IPR034197; Peptidases_S8_3.
DR InterPro; IPR010259; S8pro/Inhibitor_I9.
DR InterPro; IPR037045; S8pro/Inhibitor_I9_sf.
DR InterPro; IPR045051; SBT.
DR InterPro; IPR041469; Subtilisin-like_FN3.
DR PANTHER; PTHR10795; PTHR10795; 1.
DR Pfam; PF17766; fn3_6; 1.
DR Pfam; PF05922; Inhibitor_I9; 1.
DR Pfam; PF00082; Peptidase_S8; 1.
DR PRINTS; PR00723; SUBTILISIN.
DR SUPFAM; SSF52743; SSF52743; 1.
DR PROSITE; PS51892; SUBTILASE; 1.
DR PROSITE; PS00136; SUBTILASE_ASP; 1.
DR PROSITE; PS00137; SUBTILASE_HIS; 1.
DR PROSITE; PS00138; SUBTILASE_SER; 1.
PE 2: Evidence at transcript level;
KW Autocatalytic cleavage; Glycoprotein; Hydrolase; Protease;
KW Reference proteome; Secreted; Serine protease; Signal; Zymogen.
FT SIGNAL 1..33
FT /evidence="ECO:0000255"
FT PROPEP 34..119
FT /note="Activation peptide"
FT /evidence="ECO:0000250|UniProtKB:Q39547"
FT /id="PRO_0000435216"
FT CHAIN 120..?
FT /note="Subtilisin-like protease SBT3.16"
FT /id="PRO_0000435217"
FT PROPEP ?..759
FT /evidence="ECO:0000250|UniProtKB:Q39547"
FT /id="PRO_0000435218"
FT DOMAIN 41..118
FT /note="Inhibitor I9"
FT /evidence="ECO:0000255"
FT DOMAIN 124..608
FT /note="Peptidase S8"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01240"
FT ACT_SITE 153
FT /note="Charge relay system"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01240"
FT ACT_SITE 229
FT /note="Charge relay system"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01240"
FT ACT_SITE 539
FT /note="Charge relay system"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01240"
FT CARBOHYD 186
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 209
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 371
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 632
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 711
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
SQ SEQUENCE 759 AA; 81492 MW; 9E275F3264C4D63C CRC64;
MELSSLIVPN NKKHFVVVFI GLVLIFKIAL ITAANEKSQI YTVHLGERQH DDPNIVTESH
HDILGPLLGS KKASHESMIY SYRHGFSGFA AKLTSSQARE LSGHPDVVRV TRSKNMKLKT
TRVSDYLGLT SAAPTGLLHE TDMGSEAIVG ILDSGIWPDS KSFNDNGLGP IPTRWKGKCV
SAEAFNASSC NRKLIGAMYY SKGLESKYNG SFNAAEKGEV MSPLDKIGHG THCASTAVGS
FVPDANVLSL AQGTARGSAP RARIASYKVC WNNEECFTPD IVKAIDHAIR DGVDVLSLSL
GSEVPVDFEV DRDDFAIAAF HAVMKGIPVV CAGGNDGPEK ETISNVAPWL ITVAATTMDR
EYFTPITLGN NITLLGQEGL YIGEEVGFTD LLFYDDVTRE DMEAGKATGK ILLFFQRANF
EDDFAAYAKS KGAVGVIIAT QPTDSIDAST VDIAIAYVDN ELGMDILLYI QTTKSPIAKI
SPTKTFVGRP LATKVARFSS RGPNSLSPVI LKPDIAAPGS GILAAVPTGG GYDFMSGTSM
STPVVSGIVA LLRKKRPDWS PAAIRSALVT TALQTDPSGE PIAAEGSPRK LADPFDYGGG
LVNPVKVADP GLVYDMGHDE YVHYLCSAGY DNTSISKLLG EIYTCPTPIP SMLDVNMPSI
TIPYLSEEIT ITRTVTNVGP VGSVYKAVIQ APQGINLQVS PETLEFGSNT NKTTFTVKVS
TTHRANTDYL FGSLTWADNE GHNVRIPLSV RTRVFNFKI
