SBT41_ARATH
ID SBT41_ARATH Reviewed; 775 AA.
AC F4IG09; Q8S8T2;
DT 20-JAN-2016, integrated into UniProtKB/Swiss-Prot.
DT 28-JUN-2011, sequence version 1.
DT 03-AUG-2022, entry version 80.
DE RecName: Full=Subtilisin-like protease SBT4.1 {ECO:0000303|PubMed:16193095};
DE EC=3.4.21.- {ECO:0000255|PROSITE-ProRule:PRU10082};
DE AltName: Full=Subtilase subfamily 4 member 1 {ECO:0000303|PubMed:16193095};
DE Short=AtSBT4.1 {ECO:0000303|PubMed:16193095};
DE Flags: Precursor;
GN Name=SBT4.1 {ECO:0000303|PubMed:16193095};
GN OrderedLocusNames=At2g39850 {ECO:0000312|Araport:AT2G39850};
GN ORFNames=T5I7.15 {ECO:0000312|EMBL:AAM14853.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702 {ECO:0000312|Proteomes:UP000006548};
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=16193095; DOI=10.1371/journal.pcbi.0010040;
RA Rautengarten C., Steinhauser D., Bussis D., Stintzi A., Schaller A.,
RA Kopka J., Altmann T.;
RT "Inferring hypotheses on functional relationships of genes: Analysis of the
RT Arabidopsis thaliana subtilase gene family.";
RL PLoS Comput. Biol. 1:E40-E40(2005).
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250|UniProtKB:Q84WS0}.
CC -!- PTM: The C-terminal propeptide is autocleaved.
CC {ECO:0000250|UniProtKB:Q39547}.
CC -!- SIMILARITY: Belongs to the peptidase S8 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAM14853.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AC003000; AAM14853.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002685; AEC09738.1; -; Genomic_DNA.
DR RefSeq; NP_565915.2; NM_129544.4.
DR AlphaFoldDB; F4IG09; -.
DR SMR; F4IG09; -.
DR STRING; 3702.AT2G39850.1; -.
DR MEROPS; S08.A46; -.
DR PaxDb; F4IG09; -.
DR PRIDE; F4IG09; -.
DR ProteomicsDB; 232799; -.
DR EnsemblPlants; AT2G39850.1; AT2G39850.1; AT2G39850.
DR GeneID; 818572; -.
DR Gramene; AT2G39850.1; AT2G39850.1; AT2G39850.
DR KEGG; ath:AT2G39850; -.
DR Araport; AT2G39850; -.
DR TAIR; locus:2061131; AT2G39850.
DR eggNOG; ENOG502QRA7; Eukaryota.
DR HOGENOM; CLU_000625_4_3_1; -.
DR InParanoid; F4IG09; -.
DR OMA; CENITCN; -.
DR OrthoDB; 337164at2759; -.
DR PRO; PR:F4IG09; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; F4IG09; baseline and differential.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0004252; F:serine-type endopeptidase activity; IEA:InterPro.
DR GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR Gene3D; 3.30.70.80; -; 1.
DR Gene3D; 3.40.50.200; -; 1.
DR InterPro; IPR000209; Peptidase_S8/S53_dom.
DR InterPro; IPR036852; Peptidase_S8/S53_dom_sf.
DR InterPro; IPR022398; Peptidase_S8_His-AS.
DR InterPro; IPR023828; Peptidase_S8_Ser-AS.
DR InterPro; IPR015500; Peptidase_S8_subtilisin-rel.
DR InterPro; IPR010259; S8pro/Inhibitor_I9.
DR InterPro; IPR037045; S8pro/Inhibitor_I9_sf.
DR InterPro; IPR045051; SBT.
DR InterPro; IPR041469; Subtilisin-like_FN3.
DR PANTHER; PTHR10795; PTHR10795; 1.
DR Pfam; PF17766; fn3_6; 1.
DR Pfam; PF05922; Inhibitor_I9; 1.
DR Pfam; PF00082; Peptidase_S8; 1.
DR PRINTS; PR00723; SUBTILISIN.
DR SUPFAM; SSF52743; SSF52743; 1.
DR PROSITE; PS51892; SUBTILASE; 1.
DR PROSITE; PS00137; SUBTILASE_HIS; 1.
DR PROSITE; PS00138; SUBTILASE_SER; 1.
PE 3: Inferred from homology;
KW Autocatalytic cleavage; Glycoprotein; Hydrolase; Protease;
KW Reference proteome; Secreted; Serine protease; Signal; Zymogen.
FT SIGNAL 1..23
FT /evidence="ECO:0000255"
FT PROPEP 24..106
FT /note="Activation peptide"
FT /evidence="ECO:0000250|UniProtKB:Q39547"
FT /id="PRO_0000435225"
FT CHAIN 107..?
FT /note="Subtilisin-like protease SBT4.1"
FT /evidence="ECO:0000255"
FT /id="PRO_5003311433"
FT PROPEP ?..775
FT /evidence="ECO:0000250|UniProtKB:Q39547"
FT /id="PRO_0000435226"
FT DOMAIN 29..105
FT /note="Inhibitor I9"
FT /evidence="ECO:0000255"
FT DOMAIN 110..606
FT /note="Peptidase S8"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01240"
FT DOMAIN 365..459
FT /note="PA"
FT /evidence="ECO:0000255"
FT ACT_SITE 136
FT /note="Charge relay system"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01240"
FT ACT_SITE 196
FT /note="Charge relay system"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01240"
FT ACT_SITE 551
FT /note="Charge relay system"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01240"
FT CARBOHYD 24
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 115
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 126
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 162
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 437
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 601
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
SQ SEQUENCE 775 AA; 87920 MW; 66852498047A4973 CRC64;
MAIAFHTFLL QLLLFFFASF AEANDSRKTY LVQMKVGGHR YGSSSGHQEL LGEVLDDDST
LADAFIYSYK ESFTGFSASL TPRERQKLMR RREVLEVSRS RNLKLQTTRS WDFMNLTLKA
ERNPENESDL VVAVIDSGIW PYSELFGSDS PPPPGWENKC ENITCNNKIV GARSYYPKKE
KYKWVEEKSV IDVTGHGTHV ASIVAGRKVE KAGYFGLAEG TMRGGVPNAK IAVYKTCWRV
IRKNGREDSV CREDNILKAI DDAIADKVDI ISYSQGFQFT PLQKDKVSWA FLRALKNGIL
TSAAAGNYAN NGKFYYTVAN GAPWVMTVAA SLKDRIFETK LELEGEDKPI IVYDTINTFE
TQDSFYPLLN EKAPPESTRK RELIAERNGY SILSNYDEKD KGKDVFFEFA QINLLDEAIK
EREKGAIVLG GKSYDFNESI KLQFPIASIF LDEQKKGKLW DYYKKDQSKE RLAKIHKTEE
IPREEGWVPT VAHLSSRGPN CDSFLANILK PDIAAPGLDI IAGWPENVKL SSDRPANDYR
HLRFNIMSGT SMACPHATGL ALYLKSFKRW SPSAIKSALM TTSSEMTDDD NEFAYGSGHL
NATKVRDPGL VYETHYQDYI DYLCKLGYNT EKLRSHVGSD KIDCSKTEID HDADLNYPTM
TARVPLPLDT PFKKVFHRTV TNVNDGEFTY LREINYRGDK DFDEIIVDPP QLKFSELGET
KTFTVTVTGI SKRNWNKNRA FMTRNTWLTW TEKDGSRQVR SPIVIYSIKG PKACM
