SBT46_ARATH
ID SBT46_ARATH Reviewed; 736 AA.
AC Q9STF7;
DT 20-JAN-2016, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 141.
DE RecName: Full=Subtilisin-like protease SBT4.6 {ECO:0000303|PubMed:16193095};
DE EC=3.4.21.- {ECO:0000255|PROSITE-ProRule:PRU10082};
DE AltName: Full=Subtilase subfamily 4 member 6 {ECO:0000303|PubMed:16193095};
DE Short=AtSBT4.6 {ECO:0000303|PubMed:16193095};
DE Flags: Precursor;
GN Name=SBT4.6 {ECO:0000303|PubMed:16193095};
GN OrderedLocusNames=At3g46850 {ECO:0000312|Araport:AT3G46850};
GN ORFNames=T6H20.120 {ECO:0000312|EMBL:CAB51180.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130713; DOI=10.1038/35048706;
RA Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B.,
RA Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M.,
RA Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V.,
RA Choisne N., Artiguenave F., Robert C., Brottier P., Wincker P.,
RA Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H.,
RA Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H.,
RA Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A.,
RA Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H.,
RA Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J.,
RA Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B.,
RA Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D.,
RA de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E.,
RA Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G.,
RA Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X.,
RA Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M.,
RA Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B.,
RA Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J.,
RA Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C.,
RA Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y.,
RA Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K.,
RA Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A.,
RA Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T.,
RA Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.";
RL Nature 408:820-822(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=16193095; DOI=10.1371/journal.pcbi.0010040;
RA Rautengarten C., Steinhauser D., Bussis D., Stintzi A., Schaller A.,
RA Kopka J., Altmann T.;
RT "Inferring hypotheses on functional relationships of genes: Analysis of the
RT Arabidopsis thaliana subtilase gene family.";
RL PLoS Comput. Biol. 1:E40-E40(2005).
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250|UniProtKB:Q84WS0}.
CC -!- PTM: The C-terminal propeptide is autocleaved.
CC {ECO:0000250|UniProtKB:Q39547}.
CC -!- SIMILARITY: Belongs to the peptidase S8 family. {ECO:0000305}.
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DR EMBL; AL096859; CAB51180.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE78211.1; -; Genomic_DNA.
DR PIR; T12963; T12963.
DR RefSeq; NP_566888.2; NM_114552.3.
DR AlphaFoldDB; Q9STF7; -.
DR SMR; Q9STF7; -.
DR STRING; 3702.AT3G46850.1; -.
DR MEROPS; S08.A06; -.
DR iPTMnet; Q9STF7; -.
DR PaxDb; Q9STF7; -.
DR PRIDE; Q9STF7; -.
DR ProteomicsDB; 226670; -.
DR EnsemblPlants; AT3G46850.1; AT3G46850.1; AT3G46850.
DR GeneID; 823838; -.
DR Gramene; AT3G46850.1; AT3G46850.1; AT3G46850.
DR KEGG; ath:AT3G46850; -.
DR Araport; AT3G46850; -.
DR TAIR; locus:2102807; AT3G46850.
DR eggNOG; ENOG502QRA7; Eukaryota.
DR HOGENOM; CLU_000625_4_3_1; -.
DR InParanoid; Q9STF7; -.
DR OMA; ESRTCTK; -.
DR OrthoDB; 337164at2759; -.
DR PhylomeDB; Q9STF7; -.
DR PRO; PR:Q9STF7; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q9STF7; baseline and differential.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0004252; F:serine-type endopeptidase activity; IEA:InterPro.
DR GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR CDD; cd04852; Peptidases_S8_3; 1.
DR Gene3D; 3.30.70.80; -; 1.
DR Gene3D; 3.40.50.200; -; 1.
DR InterPro; IPR003137; PA_domain.
DR InterPro; IPR000209; Peptidase_S8/S53_dom.
DR InterPro; IPR036852; Peptidase_S8/S53_dom_sf.
DR InterPro; IPR023828; Peptidase_S8_Ser-AS.
DR InterPro; IPR015500; Peptidase_S8_subtilisin-rel.
DR InterPro; IPR034197; Peptidases_S8_3.
DR InterPro; IPR010259; S8pro/Inhibitor_I9.
DR InterPro; IPR037045; S8pro/Inhibitor_I9_sf.
DR InterPro; IPR045051; SBT.
DR InterPro; IPR041469; Subtilisin-like_FN3.
DR PANTHER; PTHR10795; PTHR10795; 1.
DR Pfam; PF17766; fn3_6; 1.
DR Pfam; PF05922; Inhibitor_I9; 1.
DR Pfam; PF02225; PA; 1.
DR Pfam; PF00082; Peptidase_S8; 1.
DR PRINTS; PR00723; SUBTILISIN.
DR SUPFAM; SSF52743; SSF52743; 1.
DR PROSITE; PS51892; SUBTILASE; 1.
DR PROSITE; PS00138; SUBTILASE_SER; 1.
PE 3: Inferred from homology;
KW Autocatalytic cleavage; Glycoprotein; Hydrolase; Protease;
KW Reference proteome; Secreted; Serine protease; Signal; Zymogen.
FT SIGNAL 1..24
FT /evidence="ECO:0000255"
FT PROPEP 25..111
FT /note="Activation peptide"
FT /evidence="ECO:0000250|UniProtKB:Q39547"
FT /id="PRO_0000435236"
FT CHAIN 112..?
FT /note="Subtilisin-like protease SBT4.6"
FT /evidence="ECO:0000255"
FT /id="PRO_5004337274"
FT PROPEP ?..736
FT /evidence="ECO:0000250|UniProtKB:Q39547"
FT /id="PRO_0000435237"
FT DOMAIN 33..110
FT /note="Inhibitor I9"
FT /evidence="ECO:0000255"
FT DOMAIN 115..589
FT /note="Peptidase S8"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01240"
FT DOMAIN 362..442
FT /note="PA"
FT /evidence="ECO:0000255"
FT ACT_SITE 143
FT /note="Charge relay system"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01240"
FT ACT_SITE 204
FT /note="Charge relay system"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01240"
FT ACT_SITE 527
FT /note="Charge relay system"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01240"
FT CARBOHYD 174
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 227
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 450
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 564
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 598
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 610
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 668
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
SQ SEQUENCE 736 AA; 78802 MW; BA3A15BC9B9F1F1D CRC64;
MATAVSYCLL SCIFALLVVS FASAGKDDQD KQVYIVYMGA LPSRVDYMPM SHHTSILQDV
TGESSIQDRL VRNYKRSFNG FAARLTESER EILASMDEVV SVFPSKNLNL QTTTSWNFMG
LKEGKRTKRN PLIESDTIIG VIDSGIYPES DSFSGKGFGP PPKKWKGVCK GGTNFTCNNK
LIGARYYTPK LEGFPESARD NTGHGSHTAS IAAGNAVKHV SFYGLGNGTV RGGVPAARIA
VYKVCDPGVI RCTSDGILAA FDDAIADKVD IITVSLGADA VGTFEEDTLA IGAFHAMAKG
ILTVNGAGNN GPERRTIVSM APWLFTVAAS NMNRAFITKV VLGNGKTIVG RSVNSFDLNG
KKYPLVYGKS ASSRCDASSA GFCSPGCLDS KRVKGKIVLC DTQRNPGEAQ AMGAVASIVR
NPYEDAASVF SFPVSVLSED DYNIVLSYVN STKNPKAAVL KSETIFNQKA PVVASYSSRG
PNPLIHDILK PDITAPGSEI LAAYSPYVPP SESDTRHVKY TVISGTSMSC PHVAGVAAYI
KTFHPLWSPS MIQSAIMTTA WPMNASTSPS NELAEFAYGA GHVDPIAAIH PGLVYEANKS
DHITFLCGFN YTGKKLRLIS GDSSSCTKEQ TKSLTRNLNY PSMSAQVSGT KPFKVTFRRT
VTNVGRPNAT YKAKVVGSKL KVKVVPAVLS LKSLYEKKSF TVTVSGAGPK AENLVSAQLI
WSDGVHFVRS PIVVYA
