SBT49_ARATH
ID SBT49_ARATH Reviewed; 713 AA.
AC Q9FIM5;
DT 20-JAN-2016, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 03-AUG-2022, entry version 136.
DE RecName: Full=Subtilisin-like protease SBT4.9 {ECO:0000303|PubMed:16193095};
DE EC=3.4.21.- {ECO:0000255|PROSITE-ProRule:PRU10082};
DE AltName: Full=Subtilase subfamily 4 member 9 {ECO:0000303|PubMed:16193095};
DE Short=AtSBT4.9 {ECO:0000303|PubMed:16193095};
DE Flags: Precursor;
GN Name=SBT4.9 {ECO:0000303|PubMed:16193095};
GN OrderedLocusNames=At5g58840 {ECO:0000312|Araport:AT5G58840};
GN ORFNames=K19M22.4 {ECO:0000312|EMBL:BAB09629.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10048488; DOI=10.1093/dnares/5.6.379;
RA Asamizu E., Sato S., Kaneko T., Nakamura Y., Kotani H., Miyajima N.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. VIII. Sequence
RT features of the regions of 1,081,958 bp covered by seventeen physically
RT assigned P1 and TAC clones.";
RL DNA Res. 5:379-391(1998).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=16193095; DOI=10.1371/journal.pcbi.0010040;
RA Rautengarten C., Steinhauser D., Bussis D., Stintzi A., Schaller A.,
RA Kopka J., Altmann T.;
RT "Inferring hypotheses on functional relationships of genes: Analysis of the
RT Arabidopsis thaliana subtilase gene family.";
RL PLoS Comput. Biol. 1:E40-E40(2005).
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250|UniProtKB:Q84WS0}.
CC -!- PTM: The C-terminal propeptide is autocleaved.
CC {ECO:0000250|UniProtKB:Q39547}.
CC -!- SIMILARITY: Belongs to the peptidase S8 family. {ECO:0000305}.
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DR EMBL; AB016885; BAB09629.1; -; Genomic_DNA.
DR EMBL; CP002688; AED97109.1; -; Genomic_DNA.
DR EMBL; BT008543; AAP40370.1; -; mRNA.
DR EMBL; BT008659; AAP40471.1; -; mRNA.
DR EMBL; AK229679; BAF01520.1; -; mRNA.
DR RefSeq; NP_568890.2; NM_125274.3.
DR AlphaFoldDB; Q9FIM5; -.
DR SMR; Q9FIM5; -.
DR STRING; 3702.AT5G58840.1; -.
DR MEROPS; S08.A07; -.
DR iPTMnet; Q9FIM5; -.
DR PaxDb; Q9FIM5; -.
DR PRIDE; Q9FIM5; -.
DR ProteomicsDB; 226650; -.
DR EnsemblPlants; AT5G58840.1; AT5G58840.1; AT5G58840.
DR GeneID; 836001; -.
DR Gramene; AT5G58840.1; AT5G58840.1; AT5G58840.
DR KEGG; ath:AT5G58840; -.
DR Araport; AT5G58840; -.
DR TAIR; locus:2154528; AT5G58840.
DR eggNOG; ENOG502QRA7; Eukaryota.
DR HOGENOM; CLU_000625_4_3_1; -.
DR InParanoid; Q9FIM5; -.
DR OMA; TISICAP; -.
DR OrthoDB; 337164at2759; -.
DR PhylomeDB; Q9FIM5; -.
DR PRO; PR:Q9FIM5; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q9FIM5; baseline and differential.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0004252; F:serine-type endopeptidase activity; IEA:InterPro.
DR GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR CDD; cd04852; Peptidases_S8_3; 1.
DR Gene3D; 3.30.70.80; -; 1.
DR Gene3D; 3.40.50.200; -; 1.
DR InterPro; IPR000209; Peptidase_S8/S53_dom.
DR InterPro; IPR036852; Peptidase_S8/S53_dom_sf.
DR InterPro; IPR023828; Peptidase_S8_Ser-AS.
DR InterPro; IPR015500; Peptidase_S8_subtilisin-rel.
DR InterPro; IPR034197; Peptidases_S8_3.
DR InterPro; IPR010259; S8pro/Inhibitor_I9.
DR InterPro; IPR037045; S8pro/Inhibitor_I9_sf.
DR InterPro; IPR045051; SBT.
DR InterPro; IPR041469; Subtilisin-like_FN3.
DR PANTHER; PTHR10795; PTHR10795; 1.
DR Pfam; PF17766; fn3_6; 1.
DR Pfam; PF05922; Inhibitor_I9; 1.
DR Pfam; PF00082; Peptidase_S8; 1.
DR PRINTS; PR00723; SUBTILISIN.
DR SUPFAM; SSF52743; SSF52743; 1.
DR PROSITE; PS51892; SUBTILASE; 1.
DR PROSITE; PS00138; SUBTILASE_SER; 1.
PE 2: Evidence at transcript level;
KW Autocatalytic cleavage; Glycoprotein; Hydrolase; Protease;
KW Reference proteome; Secreted; Serine protease; Signal; Zymogen.
FT SIGNAL 1..24
FT /evidence="ECO:0000255"
FT PROPEP 25..113
FT /note="Activation peptide"
FT /evidence="ECO:0000250|UniProtKB:Q39547"
FT /id="PRO_0000435242"
FT CHAIN 114..?
FT /note="Subtilisin-like protease SBT4.9"
FT /evidence="ECO:0000255"
FT /id="PRO_5004326411"
FT PROPEP ?..713
FT /evidence="ECO:0000250|UniProtKB:Q39547"
FT /id="PRO_0000435243"
FT DOMAIN 34..112
FT /note="Inhibitor I9"
FT /evidence="ECO:0000255"
FT DOMAIN 117..560
FT /note="Peptidase S8"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01240"
FT DOMAIN 356..415
FT /note="PA"
FT /evidence="ECO:0000255"
FT ACT_SITE 145
FT /note="Charge relay system"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01240"
FT ACT_SITE 200
FT /note="Charge relay system"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01240"
FT ACT_SITE 499
FT /note="Charge relay system"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01240"
FT CARBOHYD 176
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 215
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 223
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 420
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 536
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 583
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 627
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 637
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
SQ SEQUENCE 713 AA; 75957 MW; 38DC8864572B5707 CRC64;
MARRADSFCL ISCVLVSFVI SVSAVTDDSQ DKQVYVVYMG SLPSSRLEYT PMSHHMSILQ
EVTGESSVEG RLVRSYKRSF NGFAARLTES ERERVAEMEG VVSVFPDINY KLQTTASWDF
LGLKEGKNTK RNLAIESDTI IGFIDSGIWP ESESFSDKGF GPPPKKWKGV CSAGKNFTCN
NKLIGARDYT NEGTRDIEGH GTHTASTAAG NAVKNTSFYG IGNGTARGGV PASRIAAYKA
CSEMGCTTES VLSAFDDAIA DGVDLISISL GANLVRTYET DPIAIGAFHA MVKGILTVQS
AGNGGPNPGS VMSVAPWILT VAASNTNRGF VTKVVLGNGK TFVGKSLNAF DLKGKNYPLY
GGSTDGPLLR GKILVSEDKV SSEIVVANIN ENYHDYAYVS ILPSSALSKD DFDSVISYVN
STKSPHGTVL KSEAIFNQAA PKVAGFSSRG PNTIAVDILK PDVTAPGVEI LAAFSPLNSP
AQDKRDNRHV KYSVLSGTSM SCPHVAGVAA YIKTFHPEWS PSMIQSAIMT TAWPMNATGT
AVASTEFAYG AGHVDPIAAI NPGLVYEIGK SDHIAFLCGL NYNATSLKLI AGEAVTCTGK
TLPRNLNYPS MSAKLPKSES SFIVTFNRTV TNVGTPNSTY KSKIVLNHGS NLKVEVSPSV
LSMKSVKEKQ SFTVTVSGSN IDPKLPSSAN LIWSDGTHNV RSPIVVYTYS VSD
