SBT4A_ARATH
ID SBT4A_ARATH Reviewed; 693 AA.
AC Q9FIM8;
DT 20-JAN-2016, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 03-AUG-2022, entry version 99.
DE RecName: Full=Subtilisin-like protease SBT4.10 {ECO:0000303|PubMed:16193095};
DE EC=3.4.21.- {ECO:0000255|PROSITE-ProRule:PRU10082};
DE AltName: Full=Subtilase subfamily 4 member 10 {ECO:0000303|PubMed:16193095};
DE Short=AtSBT4.10 {ECO:0000303|PubMed:16193095};
DE Flags: Precursor;
GN Name=SBT4.10 {ECO:0000303|PubMed:16193095};
GN OrderedLocusNames=At5g58810 {ECO:0000312|Araport:AT5G58810};
GN ORFNames=K19M22.1 {ECO:0000312|EMBL:BAB09626.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10048488; DOI=10.1093/dnares/5.6.379;
RA Asamizu E., Sato S., Kaneko T., Nakamura Y., Kotani H., Miyajima N.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. VIII. Sequence
RT features of the regions of 1,081,958 bp covered by seventeen physically
RT assigned P1 and TAC clones.";
RL DNA Res. 5:379-391(1998).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=16193095; DOI=10.1371/journal.pcbi.0010040;
RA Rautengarten C., Steinhauser D., Bussis D., Stintzi A., Schaller A.,
RA Kopka J., Altmann T.;
RT "Inferring hypotheses on functional relationships of genes: Analysis of the
RT Arabidopsis thaliana subtilase gene family.";
RL PLoS Comput. Biol. 1:E40-E40(2005).
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250|UniProtKB:Q84WS0}.
CC -!- PTM: The C-terminal propeptide is autocleaved.
CC {ECO:0000250|UniProtKB:Q39547}.
CC -!- SIMILARITY: Belongs to the peptidase S8 family. {ECO:0000305}.
CC -!- CAUTION: Could be the product of a pseudogene. {ECO:0000305}.
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DR EMBL; AB016885; BAB09626.1; -; Genomic_DNA.
DR EMBL; CP002688; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR AlphaFoldDB; Q9FIM8; -.
DR SMR; Q9FIM8; -.
DR MEROPS; S08.A10; -.
DR PeptideAtlas; Q9FIM8; -.
DR PRIDE; Q9FIM8; -.
DR Araport; AT5G58810; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q9FIM8; baseline and differential.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0004252; F:serine-type endopeptidase activity; IEA:InterPro.
DR GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR CDD; cd04852; Peptidases_S8_3; 1.
DR Gene3D; 3.30.70.80; -; 1.
DR Gene3D; 3.40.50.200; -; 2.
DR InterPro; IPR000209; Peptidase_S8/S53_dom.
DR InterPro; IPR036852; Peptidase_S8/S53_dom_sf.
DR InterPro; IPR023828; Peptidase_S8_Ser-AS.
DR InterPro; IPR015500; Peptidase_S8_subtilisin-rel.
DR InterPro; IPR034197; Peptidases_S8_3.
DR InterPro; IPR010259; S8pro/Inhibitor_I9.
DR InterPro; IPR037045; S8pro/Inhibitor_I9_sf.
DR InterPro; IPR045051; SBT.
DR InterPro; IPR041469; Subtilisin-like_FN3.
DR PANTHER; PTHR10795; PTHR10795; 1.
DR Pfam; PF17766; fn3_6; 1.
DR Pfam; PF05922; Inhibitor_I9; 1.
DR Pfam; PF00082; Peptidase_S8; 1.
DR PRINTS; PR00723; SUBTILISIN.
DR SUPFAM; SSF52743; SSF52743; 1.
DR PROSITE; PS51892; SUBTILASE; 1.
DR PROSITE; PS00138; SUBTILASE_SER; 1.
PE 5: Uncertain;
KW Autocatalytic cleavage; Glycoprotein; Hydrolase; Protease;
KW Reference proteome; Secreted; Serine protease; Signal; Zymogen.
FT SIGNAL 1..25
FT /evidence="ECO:0000255"
FT PROPEP 26..113
FT /note="Activation peptide"
FT /evidence="ECO:0000250|UniProtKB:Q39547"
FT /id="PRO_0000435244"
FT CHAIN 114..?
FT /note="Subtilisin-like protease SBT4.10"
FT /evidence="ECO:0000255"
FT /id="PRO_5004325497"
FT PROPEP ?..693
FT /evidence="ECO:0000250|UniProtKB:Q39547"
FT /id="PRO_0000435245"
FT DOMAIN 35..113
FT /note="Inhibitor I9"
FT /evidence="ECO:0000255"
FT DOMAIN 117..536
FT /note="Peptidase S8"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01240"
FT DOMAIN 354..396
FT /note="PA"
FT /evidence="ECO:0000255"
FT ACT_SITE 145
FT /note="Charge relay system"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01240"
FT ACT_SITE 200
FT /note="Charge relay system"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01240"
FT ACT_SITE 475
FT /note="Charge relay system"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01240"
FT CARBOHYD 176
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 215
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 223
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 368
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 413
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 467
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 559
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 603
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 613
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
SQ SEQUENCE 693 AA; 74246 MW; C792103DA6BA7F4B CRC64;
MAKLREASFC ALACVLVLFL SFVSADTYNR QDKQVYVVYM GSLPSQPDYK PTSDHISILQ
QVTGESSMEG RLVRSYKKSF NGFSARLTES ERKRVAEMEG VVSVFPSKKY KLHTTASWDF
MGLKEGKNTK RNLAVESDTI VGVFDTGISP ESESFSGKGF GPPPKKWKGV CKGGKNFTCN
NKLIGARDYT NEGTRDIEGH GTHTASTAAG NVVENTSFYG IGNGTARGGV PDSRIAAYKV
CSGAGCSSEY ILSAFDDAIA DGVDVISASL GGDTAYMYEK DPIAIGAFHA MAKGILTVQS
AGNNGPNPTV SVAPWILTVA ASTTNRRIVT KVVLGNGKTL VGQSVNAFDL KGKQYPLVYE
TSVEKCNNES LTTLALSFLT LTPQSNEQII SMFHTLIMWS PKATILKSEA IFNQTDPKVA
GFSSRGPNTI AVDILKPDIT APGVEILAAY SPLVSPSATT LDNRRVNYTI TSGTSMACPH
VSGVAAYIKT FHPEWYPSMI QSAIMTTAWP MNPSGTDAVS TEFAYGSGHI DPIAAINPGL
VYELGKSDHI AFLCGLNYNA TTLKLIAGEA VTCTGKTLPR NLNYPSMSAK LSKSKSSFTV
TFNRTVTNVG TSNSTYKSKV VINHGSKLKV KVSPSVLSMK SVNEKQSFTV SVSGNDLNPK
LPSSANLIWS DGTHNVRSPI VVYTDYASSV DIF
