SBT4F_ARATH
ID SBT4F_ARATH Reviewed; 766 AA.
AC Q9LZS6;
DT 20-JAN-2016, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 142.
DE RecName: Full=Subtilisin-like protease SBT4.15 {ECO:0000303|PubMed:16193095};
DE EC=3.4.21.- {ECO:0000255|PROSITE-ProRule:PRU10082};
DE AltName: Full=Subtilase subfamily 4 member 15 {ECO:0000303|PubMed:16193095};
DE Short=AtSBT4.15 {ECO:0000303|PubMed:16193095};
DE Flags: Precursor;
GN Name=SBT4.15 {ECO:0000303|PubMed:16193095};
GN OrderedLocusNames=At5g03620 {ECO:0000312|Araport:AT5G03620};
GN ORFNames=F17C15.40 {ECO:0000312|EMBL:CAB82927.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130714; DOI=10.1038/35048507;
RA Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E.,
RA Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M.,
RA Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K.,
RA Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M.,
RA Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L.,
RA O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D.,
RA Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M.,
RA Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L.,
RA Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B.,
RA Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P.,
RA Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M.,
RA Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D.,
RA Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A.,
RA Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I.,
RA Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T.,
RA Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A.,
RA McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U.,
RA Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W.,
RA Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M.,
RA Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S.,
RA Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G.,
RA Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W.,
RA Bevan M., Fransz P.F.;
RT "Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.";
RL Nature 408:823-826(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=16193095; DOI=10.1371/journal.pcbi.0010040;
RA Rautengarten C., Steinhauser D., Bussis D., Stintzi A., Schaller A.,
RA Kopka J., Altmann T.;
RT "Inferring hypotheses on functional relationships of genes: Analysis of the
RT Arabidopsis thaliana subtilase gene family.";
RL PLoS Comput. Biol. 1:E40-E40(2005).
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250|UniProtKB:Q84WS0}.
CC -!- PTM: The C-terminal propeptide is autocleaved.
CC {ECO:0000250|UniProtKB:Q39547}.
CC -!- SIMILARITY: Belongs to the peptidase S8 family. {ECO:0000305}.
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DR EMBL; AL162506; CAB82927.1; -; Genomic_DNA.
DR EMBL; CP002688; AED90634.1; -; Genomic_DNA.
DR PIR; T48389; T48389.
DR RefSeq; NP_568124.1; NM_120443.2.
DR AlphaFoldDB; Q9LZS6; -.
DR SMR; Q9LZS6; -.
DR STRING; 3702.AT5G03620.1; -.
DR MEROPS; S08.A13; -.
DR iPTMnet; Q9LZS6; -.
DR PaxDb; Q9LZS6; -.
DR PRIDE; Q9LZS6; -.
DR ProteomicsDB; 232737; -.
DR EnsemblPlants; AT5G03620.1; AT5G03620.1; AT5G03620.
DR GeneID; 831777; -.
DR Gramene; AT5G03620.1; AT5G03620.1; AT5G03620.
DR KEGG; ath:AT5G03620; -.
DR Araport; AT5G03620; -.
DR TAIR; locus:2144583; AT5G03620.
DR eggNOG; ENOG502QRA7; Eukaryota.
DR HOGENOM; CLU_000625_4_3_1; -.
DR InParanoid; Q9LZS6; -.
DR OMA; MGEDKVM; -.
DR OrthoDB; 337164at2759; -.
DR PhylomeDB; Q9LZS6; -.
DR PRO; PR:Q9LZS6; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q9LZS6; baseline and differential.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0004252; F:serine-type endopeptidase activity; IEA:InterPro.
DR GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR CDD; cd04852; Peptidases_S8_3; 1.
DR Gene3D; 3.30.70.80; -; 1.
DR Gene3D; 3.40.50.200; -; 1.
DR InterPro; IPR000209; Peptidase_S8/S53_dom.
DR InterPro; IPR036852; Peptidase_S8/S53_dom_sf.
DR InterPro; IPR023828; Peptidase_S8_Ser-AS.
DR InterPro; IPR015500; Peptidase_S8_subtilisin-rel.
DR InterPro; IPR034197; Peptidases_S8_3.
DR InterPro; IPR010259; S8pro/Inhibitor_I9.
DR InterPro; IPR037045; S8pro/Inhibitor_I9_sf.
DR InterPro; IPR045051; SBT.
DR InterPro; IPR041469; Subtilisin-like_FN3.
DR PANTHER; PTHR10795; PTHR10795; 1.
DR Pfam; PF17766; fn3_6; 1.
DR Pfam; PF05922; Inhibitor_I9; 1.
DR Pfam; PF00082; Peptidase_S8; 1.
DR PRINTS; PR00723; SUBTILISIN.
DR SUPFAM; SSF52743; SSF52743; 1.
DR PROSITE; PS51892; SUBTILASE; 1.
DR PROSITE; PS00138; SUBTILASE_SER; 1.
PE 3: Inferred from homology;
KW Autocatalytic cleavage; Glycoprotein; Hydrolase; Protease;
KW Reference proteome; Secreted; Serine protease; Signal; Zymogen.
FT SIGNAL 1..23
FT /evidence="ECO:0000255"
FT PROPEP 24..113
FT /note="Activation peptide"
FT /evidence="ECO:0000250|UniProtKB:Q39547"
FT /id="PRO_0000435253"
FT CHAIN 114..?
FT /note="Subtilisin-like protease SBT4.15"
FT /evidence="ECO:0000255"
FT /id="PRO_5004329362"
FT PROPEP ?..766
FT /evidence="ECO:0000250|UniProtKB:Q39547"
FT /id="PRO_0000435254"
FT DOMAIN 35..113
FT /note="Inhibitor I9"
FT /evidence="ECO:0000255"
FT DOMAIN 117..601
FT /note="Peptidase S8"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01240"
FT DOMAIN 365..460
FT /note="PA"
FT /evidence="ECO:0000255"
FT ACT_SITE 144
FT /note="Charge relay system"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01240"
FT ACT_SITE 210
FT /note="Charge relay system"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01240"
FT ACT_SITE 543
FT /note="Charge relay system"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01240"
FT CARBOHYD 175
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 233
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 376
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 465
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 624
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 638
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 668
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
SQ SEQUENCE 766 AA; 82595 MW; 41D51BABC3EE2120 CRC64;
MVSNQRVRLF MLCFCLVNNA VIAATEDENV ERKPYIVYMG EATENSLVEA AENHHNLLMT
VIGDESKARE LKIYSYGKNI NGFVARLFPH EAEKLSREEG VVSVFKNTQR QLHTTRSWDF
LGLVESKYKR SVGIESNIIV GVLDTGIDVE SPSFNDKGVG PPPAKWKGKC VTGNNFTRCN
NKVIGAKYFH IQSEGLPDGE GDTAADHDGH GTHTSSTIAG VSVSSASLFG IANGTARGGV
PSARIAAYKV CWDSGCTDMD MLAAFDEAIS DGVDIISISI GGASLPFFED PIAIGAFHAM
KRGILTTCSA GNNGPGLFTV SNLAPWVMTV AANSLDRKFE TVVKLGNGLT ASGISLNGFN
PRKKMYPLTS GSLASNLSAG GYGEPSTCEP GTLGEDKVMG KVVYCEAGRE EGGNGGQGQD
HVVRSLKGAG VIVQLLEPTD MATSTLIAGS YVFFEDGTKI TEYINSTKNP QAVIFKTKTT
KMLAPSISSF SARGPQRISP NILKPDISAP GLNILAAYSK LASVTGYPDD NRRTLFSIMS
GTSMACPHAA AAAAYVKSFH PDWSPAAIKS ALMTTATPMR IKGNEAELSY GSGQINPRRA
IHPGLVYDIT EDAYLRFLCK EGYNSTSIGL LTGDNSNNTT KKEYNCENIK RGLGSDGLNY
PSLHKQVNST EAKVSEVFYR TVTNVGYGPS TYVARVWAPK GLRVEVVPKV MSFERPKEKR
NFKVVIDGVW DETMKGIVSA SVEWDDSRGH LVRSPILLFR SDNDYR
