SBTXB_SOYBN
ID SBTXB_SOYBN Reviewed; 25 AA.
AC P86548;
DT 13-JUL-2010, integrated into UniProtKB/Swiss-Prot.
DT 13-JUL-2010, sequence version 1.
DT 25-MAY-2022, entry version 23.
DE RecName: Full=Soybean toxin 17 kDa chain {ECO:0000303|PubMed:18328522};
DE Short=SBTX 17 kDa chain {ECO:0000303|PubMed:18328522};
DE AltName: Full=Cyclophilin-like protein;
DE Flags: Fragment;
OS Glycine max (Soybean) (Glycine hispida).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine;
OC Glycine subgen. Soja.
OX NCBI_TaxID=3847;
RN [1] {ECO:0000305}
RP PROTEIN SEQUENCE, FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, SUBUNIT,
RP GLYCOSYLATION, AND TOXIC DOSE.
RC STRAIN=cv. BR-10 {ECO:0000269|PubMed:18328522};
RC TISSUE=Seed {ECO:0000269|PubMed:18328522};
RX PubMed=18328522; DOI=10.1016/j.toxicon.2007.10.005;
RA Vasconcelos I.M., Morais J.K., Siebra E.A., Carlini C.R., Sousa D.O.,
RA Beltramini L.M., Melo V.M., Oliveira J.T.;
RT "SBTX, a new toxic protein distinct from soyatoxin and other toxic soybean
RT [Glycine max] proteins, and its inhibitory effect on Cercospora sojina
RT growth.";
RL Toxicon 51:952-963(2008).
RN [2] {ECO:0000305}
RP TISSUE SPECIFICITY, AND INDUCTION.
RA Oliveira H.D.;
RL Submitted (MAR-2010) to UniProtKB.
RN [3]
RP PARTIAL PROTEIN SEQUENCE, FUNCTION, AND TOXIC DOSE.
RC TISSUE=Seed {ECO:0000303|PubMed:20831249};
RX PubMed=20831249; DOI=10.1021/jf101688k;
RA Morais J.K., Gomes V.M., Oliveira J.T., Santos I.S., Da Cunha M.,
RA Oliveira H.D., Oliveira H.P., Sousa D.O., Vasconcelos I.M.;
RT "Soybean toxin (SBTX), a protein from soybeans that inhibits the life cycle
RT of plant and human pathogenic fungi.";
RL J. Agric. Food Chem. 58:10356-10363(2010).
RN [4]
RP FUNCTION.
RX PubMed=23894655; DOI=10.1371/journal.pone.0070425;
RA Morais J.K., Bader O., Weig M., Oliveira J.T., Arantes M.R., Gomes V.M.,
RA Da Cunha M., Oliveira H.D., Sousa D.O., Lourencao A.L., Vasconcelos I.M.;
RT "Soybean toxin (SBTX) impairs fungal growth by interfering with molecular
RT transport, carbohydrate/amino acid metabolism and drug/stress responses.";
RL PLoS ONE 8:E70425-E70425(2013).
CC -!- FUNCTION: Involved in plant defense (PubMed:18328522). Inhibits spore
CC germination in C.sojina, A.niger (at concentrations >50 ug/ml) and
CC P.herguei but not in F.oxysporum and F.solani (PubMed:20831249,
CC PubMed:18328522). Does not inhibit vegetative mycelial growth
CC (PubMed:20831249). Inhibits growth of C.albicans and K.marxiannus but
CC not P.membranifaciens or C.parapsilosis (PubMed:20831249,
CC PubMed:23894655). Probably acts by affecting the cell membrane
CC (PubMed:20831249). Does not have urease, chitinase, beta-1,3-glucanase
CC or hemagglutination activities (PubMed:18328522, PubMed:20831249). Does
CC not inhibit trypsin (PubMed:18328522). Injection into mice produces
CC toxic effects such as dyspnea, tonic-clonic convulsion and death
CC (PubMed:18328522). {ECO:0000269|PubMed:18328522,
CC ECO:0000269|PubMed:20831249, ECO:0000269|PubMed:23894655}.
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC pH dependence:
CC Activity decreases below pH 5.5 and above pH 8.0.
CC {ECO:0000269|PubMed:18328522};
CC -!- SUBUNIT: Heterodimer of a 27 kDa subunit and a 17 kDa subunit;
CC disulfide-linked. {ECO:0000269|PubMed:18328522}.
CC -!- TISSUE SPECIFICITY: Expressed in seeds, leaves, roots and stem (at
CC protein level). {ECO:0000269|Ref.2}.
CC -!- INDUCTION: In seeds, induced by jasmonate. {ECO:0000269|Ref.2}.
CC -!- PTM: SBTX is known to be glycosylated but it is not known which one of
CC its two subunits is modified; contains 5% carbohydrates.
CC {ECO:0000269|PubMed:18328522}.
CC -!- TOXIC DOSE: LD(50) is 5.6 mg/kg by intraperitoneal injection into mice.
CC {ECO:0000269|PubMed:18328522, ECO:0000269|PubMed:20831249}.
CC -!- MISCELLANEOUS: On the 2D-gel the determined pI of SBTX is: 8.2, its MW
CC is: 44 kDa. {ECO:0000269|PubMed:18328522}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR AlphaFoldDB; P86548; -.
DR SMR; P86548; -.
DR Proteomes; UP000008827; Unplaced.
DR GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR GO; GO:0050832; P:defense response to fungus; IEA:UniProtKB-KW.
DR GO; GO:0031640; P:killing of cells of another organism; IEA:UniProtKB-KW.
PE 1: Evidence at protein level;
KW Antimicrobial; Direct protein sequencing; Disulfide bond; Fungicide;
KW Plant defense; Reference proteome; Toxin.
FT CHAIN 1..>25
FT /note="Soybean toxin 17 kDa chain"
FT /id="PRO_0000395398"
FT NON_TER 25
FT /evidence="ECO:0000303|PubMed:18328522"
SQ SEQUENCE 25 AA; 2758 MW; E9ABBB355CA4202E CRC64;
PNPKVFFDMT IGGQSAGRIV MEEYA
