SC13B_ARATH
ID SC13B_ARATH Reviewed; 302 AA.
AC O64740; Q8LAX1;
DT 29-OCT-2014, integrated into UniProtKB/Swiss-Prot.
DT 01-AUG-1998, sequence version 1.
DT 25-MAY-2022, entry version 157.
DE RecName: Full=Protein transport protein SEC13 homolog B {ECO:0000305};
DE AltName: Full=SEC13-like protein B {ECO:0000305};
GN Name=SEC13B {ECO:0000303|PubMed:24280388};
GN Synonyms=SEC13 {ECO:0000303|PubMed:21189294};
GN OrderedLocusNames=At2g30050 {ECO:0000312|Araport:AT2G30050};
GN ORFNames=T27E13.21 {ECO:0000305};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP IDENTIFICATION IN THE NUCLEAR PORE COMPLEX BY MASS SPECTROMETRY,
RP SUBCELLULAR LOCATION, AND NOMENCLATURE.
RX PubMed=21189294; DOI=10.1105/tpc.110.079947;
RA Tamura K., Fukao Y., Iwamoto M., Haraguchi T., Hara-Nishimura I.;
RT "Identification and characterization of nuclear pore complex components in
RT Arabidopsis thaliana.";
RL Plant Cell 22:4084-4097(2010).
RN [6]
RP IDENTIFICATION BY MASS SPECTROMETRY, AND INTERACTION WITH MAG5; SEC31A AND
RP SEC31B.
RX PubMed=24280388; DOI=10.1105/tpc.113.118158;
RA Takagi J., Renna L., Takahashi H., Koumoto Y., Tamura K., Stefano G.,
RA Fukao Y., Kondo M., Nishimura M., Shimada T., Brandizzi F.,
RA Hara-Nishimura I.;
RT "MAIGO5 functions in protein export from Golgi-associated endoplasmic
RT reticulum exit sites in Arabidopsis.";
RL Plant Cell 25:4658-4675(2013).
CC -!- FUNCTION: Required for protein transport from the endoplasmic reticulum
CC to the Golgi apparatus. {ECO:0000305|PubMed:24280388}.
CC -!- SUBUNIT: Part of the nuclear pore complex (NPC). The NPC has an eight-
CC fold symmetrical structure comprising a central transport channel and
CC two rings, the cytoplasmic and nuclear rings, to which eight filaments
CC are attached. The cytoplasmic filaments have loose ends, while the
CC nuclear filaments are joined in a distal ring, forming a nuclear
CC basket. NPCs are highly dynamic in configuration and composition, and
CC can be devided in 3 subcomplexes, the NUP62 subcomplex, the NUP107-160
CC subcomplex and the NUP93 subcomplex, containing approximately 30
CC different nucleoporin proteins. Interacts with MAG5, SEC31A and SEC31B.
CC {ECO:0000269|PubMed:24280388, ECO:0000305|PubMed:21189294}.
CC -!- SUBCELLULAR LOCATION: Golgi apparatus {ECO:0000305|PubMed:24280388}.
CC Endoplasmic reticulum {ECO:0000269|PubMed:21189294,
CC ECO:0000305|PubMed:24280388}. Nucleus envelope
CC {ECO:0000269|PubMed:21189294}. Nucleus, nuclear pore complex
CC {ECO:0000305|PubMed:21189294}.
CC -!- SIMILARITY: Belongs to the WD repeat SEC13 family. {ECO:0000305}.
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DR EMBL; AC004165; AAC16967.1; -; Genomic_DNA.
DR EMBL; AC004680; AAM14986.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC08339.1; -; Genomic_DNA.
DR EMBL; AF412112; AAL06565.1; -; mRNA.
DR EMBL; AY124848; AAM70557.1; -; mRNA.
DR EMBL; AY087553; AAM65095.1; -; mRNA.
DR PIR; T00593; T02480.
DR RefSeq; NP_180566.1; NM_128560.3.
DR AlphaFoldDB; O64740; -.
DR SMR; O64740; -.
DR BioGRID; 2905; 12.
DR IntAct; O64740; 4.
DR STRING; 3702.AT2G30050.1; -.
DR PaxDb; O64740; -.
DR PRIDE; O64740; -.
DR ProMEX; O64740; -.
DR ProteomicsDB; 232884; -.
DR EnsemblPlants; AT2G30050.1; AT2G30050.1; AT2G30050.
DR GeneID; 817556; -.
DR Gramene; AT2G30050.1; AT2G30050.1; AT2G30050.
DR KEGG; ath:AT2G30050; -.
DR Araport; AT2G30050; -.
DR TAIR; locus:2045648; AT2G30050.
DR eggNOG; KOG1332; Eukaryota.
DR HOGENOM; CLU_032441_0_1_1; -.
DR InParanoid; O64740; -.
DR OMA; DEVTFWK; -.
DR OrthoDB; 944756at2759; -.
DR PhylomeDB; O64740; -.
DR PRO; PR:O64740; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; O64740; baseline and differential.
DR Genevisible; O64740; AT.
DR GO; GO:0030127; C:COPII vesicle coat; IBA:GO_Central.
DR GO; GO:0005783; C:endoplasmic reticulum; IEA:UniProtKB-SubCell.
DR GO; GO:0005794; C:Golgi apparatus; IEA:UniProtKB-SubCell.
DR GO; GO:0005635; C:nuclear envelope; IDA:TAIR.
DR GO; GO:0005643; C:nuclear pore; IDA:TAIR.
DR GO; GO:0031080; C:nuclear pore outer ring; IBA:GO_Central.
DR GO; GO:0005730; C:nucleolus; HDA:TAIR.
DR GO; GO:0005198; F:structural molecule activity; IBA:GO_Central.
DR GO; GO:0090114; P:COPII-coated vesicle budding; IBA:GO_Central.
DR GO; GO:0051028; P:mRNA transport; IEA:UniProtKB-KW.
DR GO; GO:0006606; P:protein import into nucleus; IBA:GO_Central.
DR Gene3D; 2.130.10.10; -; 1.
DR InterPro; IPR020472; G-protein_beta_WD-40_rep.
DR InterPro; IPR037363; Sec13/Seh1_fam.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR InterPro; IPR001680; WD40_repeat.
DR InterPro; IPR036322; WD40_repeat_dom_sf.
DR PANTHER; PTHR11024; PTHR11024; 1.
DR Pfam; PF00400; WD40; 5.
DR PRINTS; PR00320; GPROTEINBRPT.
DR SMART; SM00320; WD40; 6.
DR SUPFAM; SSF50978; SSF50978; 1.
DR PROSITE; PS50082; WD_REPEATS_2; 3.
DR PROSITE; PS50294; WD_REPEATS_REGION; 1.
PE 1: Evidence at protein level;
KW Endoplasmic reticulum; ER-Golgi transport; Golgi apparatus; mRNA transport;
KW Nuclear pore complex; Nucleus; Protein transport; Reference proteome;
KW Repeat; Translocation; Transport; WD repeat.
FT CHAIN 1..302
FT /note="Protein transport protein SEC13 homolog B"
FT /id="PRO_0000430539"
FT REPEAT 9..48
FT /note="WD 1"
FT /evidence="ECO:0000255"
FT REPEAT 54..95
FT /note="WD 2"
FT /evidence="ECO:0000255"
FT REPEAT 101..142
FT /note="WD 3"
FT /evidence="ECO:0000255"
FT REPEAT 148..201
FT /note="WD 4"
FT /evidence="ECO:0000255"
FT REPEAT 208..251
FT /note="WD 5"
FT /evidence="ECO:0000255"
FT REPEAT 257..296
FT /note="WD 6"
FT /evidence="ECO:0000255"
FT CONFLICT 267
FT /note="W -> S (in Ref. 4; AAM65095)"
SQ SEQUENCE 302 AA; 32642 MW; F9D1C36D4F98C8EF CRC64;
MPGQKIETGH EDIVHDVQMD YYGKRIATAS SDCTIKITGV SNNGGSQQLA TLTGHRGPVW
EVAWAHPKYG SILASCSYDG QVILWKEGNQ NQWTQDHVFT DHKSSVNSIA WAPHDIGLSL
ACGSSDGNIS VFTARADGGW DTSRIDQAHP VGVTSVSWAP ATAPGALVSS GLLDPVYKLA
SGGCDNTVKV WKLANGSWKM DCFPALQKHT DWVRDVAWAP NLGLPKSTIA SGSQDGKVII
WTVGKEGEQW EGKVLKDFMT PVWRVSWSLT GNLLAVSDGN NNVTVWKEAV DGEWEQVTAV
EP
