SC15A_ARATH
ID SC15A_ARATH Reviewed; 790 AA.
AC Q9LXX6;
DT 25-OCT-2002, integrated into UniProtKB/Swiss-Prot.
DT 31-MAY-2011, sequence version 2.
DT 25-MAY-2022, entry version 121.
DE RecName: Full=Exocyst complex component SEC15A;
DE Short=AtSec15a;
DE AltName: Full=Probable exocyst complex component 6;
GN Name=SEC15A; OrderedLocusNames=At3g56640; ORFNames=T5P19.290;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130713; DOI=10.1038/35048706;
RA Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B.,
RA Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M.,
RA Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V.,
RA Choisne N., Artiguenave F., Robert C., Brottier P., Wincker P.,
RA Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H.,
RA Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H.,
RA Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A.,
RA Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H.,
RA Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J.,
RA Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B.,
RA Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D.,
RA de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E.,
RA Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G.,
RA Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X.,
RA Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M.,
RA Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B.,
RA Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J.,
RA Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C.,
RA Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y.,
RA Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K.,
RA Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A.,
RA Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T.,
RA Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.";
RL Nature 408:820-822(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP COMPONENT OF THE EXOCYST COMPLEX, AND DISRUPTION PHENOTYPE.
RX PubMed=18492870; DOI=10.1105/tpc.108.059105;
RA Hala M., Cole R., Synek L., Drdova E., Pecenkova T., Nordheim A.,
RA Lamkemeyer T., Madlung J., Hochholdinger F., Fowler J.E., Zarsky V.;
RT "An exocyst complex functions in plant cell growth in Arabidopsis and
RT tobacco.";
RL Plant Cell 20:1330-1345(2008).
RN [4]
RP GENE FAMILY, NOMENCLATURE, AND SUBCELLULAR LOCATION.
RX PubMed=19895414; DOI=10.1111/j.1469-8137.2009.03070.x;
RA Chong Y.T., Gidda S.K., Sanford C., Parkinson J., Mullen R.T., Goring D.R.;
RT "Characterization of the Arabidopsis thaliana exocyst complex gene families
RT by phylogenetic, expression profiling, and subcellular localization
RT studies.";
RL New Phytol. 185:401-419(2010).
CC -!- FUNCTION: Component of the exocyst complex involved in the docking of
CC exocytic vesicles with fusion sites on the plasma membrane during
CC regulated or polarized secretion. Involved in polarized cell growth and
CC organ morphogenesis. During cytokinesis, involved in cell plate
CC initiation, cell plate maturation and formation of new primary cell
CC wall.
CC -!- SUBUNIT: The exocyst complex is composed of SEC3, SEC5, SEC6, SEC8,
CC SEC10, EXO70A1 and EXO84B.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytosol {ECO:0000269|PubMed:19895414}.
CC Note=Localized to globular structures in the perinuclear region.
CC -!- DISRUPTION PHENOTYPE: Male gametophytic lethal due to defect in pollen
CC germination and pollen tube growth. {ECO:0000269|PubMed:18492870}.
CC -!- SIMILARITY: Belongs to the SEC15 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAB88067.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR EMBL; AL163972; CAB88067.1; ALT_INIT; Genomic_DNA.
DR EMBL; CP002686; AEE79547.1; -; Genomic_DNA.
DR PIR; T49065; T49065.
DR RefSeq; NP_191223.2; NM_115523.3.
DR AlphaFoldDB; Q9LXX6; -.
DR SMR; Q9LXX6; -.
DR STRING; 3702.AT3G56640.1; -.
DR TCDB; 1.F.2.1.3; the octameric exocyst (exocyst) family.
DR PaxDb; Q9LXX6; -.
DR PRIDE; Q9LXX6; -.
DR ProteomicsDB; 232960; -.
DR EnsemblPlants; AT3G56640.1; AT3G56640.1; AT3G56640.
DR GeneID; 824831; -.
DR Gramene; AT3G56640.1; AT3G56640.1; AT3G56640.
DR KEGG; ath:AT3G56640; -.
DR Araport; AT3G56640; -.
DR TAIR; locus:2102644; AT3G56640.
DR eggNOG; KOG2176; Eukaryota.
DR HOGENOM; CLU_019671_0_0_1; -.
DR InParanoid; Q9LXX6; -.
DR OMA; RDHYNEV; -.
DR OrthoDB; 185623at2759; -.
DR PRO; PR:Q9LXX6; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q9LXX6; baseline and differential.
DR Genevisible; Q9LXX6; AT.
DR GO; GO:0005829; C:cytosol; IDA:TAIR.
DR GO; GO:0000145; C:exocyst; IDA:TAIR.
DR GO; GO:0060321; P:acceptance of pollen; IGI:TAIR.
DR GO; GO:0006887; P:exocytosis; IBA:GO_Central.
DR GO; GO:0006893; P:Golgi to plasma membrane transport; IBA:GO_Central.
DR GO; GO:0006886; P:intracellular protein transport; IEA:InterPro.
DR GO; GO:0009846; P:pollen germination; IMP:TAIR.
DR GO; GO:0009860; P:pollen tube growth; IMP:TAIR.
DR GO; GO:0090522; P:vesicle tethering involved in exocytosis; IEA:InterPro.
DR Gene3D; 1.10.357.30; -; 1.
DR Gene3D; 1.20.58.670; -; 1.
DR InterPro; IPR007225; EXOC6/Sec15.
DR InterPro; IPR042045; EXOC6/Sec15_C_dom1.
DR InterPro; IPR042044; EXOC6PINT-1/Sec15/Tip20_C_dom2.
DR InterPro; IPR046361; Sec15_C.
DR PANTHER; PTHR12702; PTHR12702; 1.
DR Pfam; PF04091; Sec15; 1.
DR PIRSF; PIRSF025007; Sec15; 1.
PE 3: Inferred from homology;
KW Coiled coil; Cytoplasm; Exocytosis; Reference proteome; Transport.
FT CHAIN 1..790
FT /note="Exocyst complex component SEC15A"
FT /id="PRO_0000118957"
FT COILED 49..70
FT /evidence="ECO:0000255"
SQ SEQUENCE 790 AA; 90000 MW; 0CBC0225610B24DA CRC64;
MMEAKPKRRI VTENGDTGED LVLATLIGNG DDVGPLVRHA FEMGRPEPLV HQLKNVARKK
EAEIEDLCKT HYEEFIVAVD ELRGVLVDAE ELKSDLASDN FRLQEVGSAL LVKLEELLES
YAVKKNVTEA IKMSKICVQA LELCVKCNSY ISEGQFYHAL KTMDLIEKSY LKLIPLKVLK
LVIERRIPVI KTHIEKKVCS QFNEWLVHIR SSSKNIGQTA IGLTASARQR EEEMLERQRR
AEEQNTGGLG ELAYTLDVED SEQDSVLKFD LTPLYRAYHI HTILGVPERF RDYYYENRLL
QLQSDLQITY TQPFVESYQT FLAQVAGYFI VEDRVIRTAG DFLLADQVET MWETAISKIV
AILENQFARM DSPTHLLLVK DYVTLLGTTL RQYGYEVGPV LDALDKSRDK YHELLLEECR
KQIVTAITED TYQQMVIKKE ADYENNVLSF NLQTSDIMPA FTYIAPFSSM VPDVCRIIRS
YIKGSVDYLS YGVNTNFFSV LRKYLDKILI DVLNEVILET ISNNSIGVSQ AMQIAANISF
LEKASDYFLR HAAQLCGIPS RSVERPQASL AAKVVLKTSR DAAYLALLNV VNTKLDEFMK
LTENVNWTTE EMPQGPHEYI NEVVIYLETV MSTAQQILPM DALYKVGVGA IEHISNSIVS
TFLSDSIKRF NANAVSAINH DLRVIENFAD ERYHSSGLNE IYKEGSFRSY LVEARQLINL
LSSSQPENFM NPVIRERNYN TLDYKKVATI CEKFKDSADG IFGSLANRNT KLTAKKKSMD
MLKKRLKEFN
