SC15B_ARATH
ID SC15B_ARATH Reviewed; 787 AA.
AC F4JHH5; O81298;
DT 11-DEC-2013, integrated into UniProtKB/Swiss-Prot.
DT 28-JUN-2011, sequence version 1.
DT 25-MAY-2022, entry version 72.
DE RecName: Full=Exocyst complex component SEC15B {ECO:0000303|PubMed:19895414};
DE Short=AtSec15b {ECO:0000303|PubMed:19895414};
GN Name=SEC15B {ECO:0000303|PubMed:19895414};
GN OrderedLocusNames=At4g02350 {ECO:0000312|Araport:AT4G02350};
GN ORFNames=T14P8.16 {ECO:0000312|EMBL:AAC19268.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617198; DOI=10.1038/47134;
RA Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T.,
RA Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B.,
RA Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M.,
RA de Simone V., Obermaier B., Mache R., Mueller M., Kreis M., Delseny M.,
RA Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D.,
RA Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J.,
RA Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B.,
RA Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J.,
RA Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R.,
RA Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M.,
RA Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P.,
RA Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S.,
RA Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C.,
RA Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J.,
RA Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S.,
RA Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A.,
RA Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M.,
RA Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D.,
RA Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E.,
RA Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S.,
RA Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R.,
RA Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M.,
RA Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E.,
RA Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P.,
RA Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K.,
RA Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K.,
RA de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K.,
RA Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M.,
RA Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G.,
RA Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K.,
RA Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K.,
RA Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W.,
RA Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H.,
RA Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B.,
RA Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J.,
RA Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K.,
RA O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N.,
RA Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A.,
RA Martienssen R., McCombie W.R.;
RT "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.";
RL Nature 402:769-777(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP COMPONENT OF THE EXOCYST COMPLEX.
RX PubMed=18492870; DOI=10.1105/tpc.108.059105;
RA Hala M., Cole R., Synek L., Drdova E., Pecenkova T., Nordheim A.,
RA Lamkemeyer T., Madlung J., Hochholdinger F., Fowler J.E., Zarsky V.;
RT "An exocyst complex functions in plant cell growth in Arabidopsis and
RT tobacco.";
RL Plant Cell 20:1330-1345(2008).
RN [4]
RP GENE FAMILY, NOMENCLATURE, AND SUBCELLULAR LOCATION.
RX PubMed=19895414; DOI=10.1111/j.1469-8137.2009.03070.x;
RA Chong Y.T., Gidda S.K., Sanford C., Parkinson J., Mullen R.T., Goring D.R.;
RT "Characterization of the Arabidopsis thaliana exocyst complex gene families
RT by phylogenetic, expression profiling, and subcellular localization
RT studies.";
RL New Phytol. 185:401-419(2010).
RN [5]
RP FUNCTION, INTERACTION WITH EXO84B, AND SUBCELLULAR LOCATION.
RX PubMed=20870962; DOI=10.1105/tpc.110.074351;
RA Fendrych M., Synek L., Pecenkova T., Toupalova H., Cole R., Drdova E.,
RA Nebesarova J., Sedinova M., Hala M., Fowler J.E., Zarsky V.;
RT "The Arabidopsis exocyst complex is involved in cytokinesis and cell plate
RT maturation.";
RL Plant Cell 22:3053-3065(2010).
RN [6]
RP INTERACTION WITH EXO70H1 AND EXO70B2.
RC STRAIN=cv. Columbia;
RX PubMed=21199889; DOI=10.1093/jxb/erq402;
RA Pecenkova T., Hala M., Kulich I., Kocourkova D., Drdova E., Fendrych M.,
RA Toupalova H., Zarsky V.;
RT "The role for the exocyst complex subunits Exo70B2 and Exo70H1 in the
RT plant-pathogen interaction.";
RL J. Exp. Bot. 62:2107-2116(2011).
RN [7]
RP SUBCELLULAR LOCATION.
RC STRAIN=cv. Columbia;
RX PubMed=24307681; DOI=10.1091/mbc.e13-10-0586;
RA Ding Y., Wang J., Chun Lai J.H., Ling Chan V.H., Wang X., Cai Y., Tan X.,
RA Bao Y., Xia J., Robinson D.G., Jiang L.;
RT "Exo70E2 is essential for exocyst subunit recruitment and EXPO formation in
RT both plants and animals.";
RL Mol. Biol. Cell 25:412-426(2014).
CC -!- FUNCTION: Component of the exocyst complex involved in the docking of
CC exocytic vesicles with fusion sites on the plasma membrane during
CC regulated or polarized secretion. Involved in polarized cell growth and
CC organ morphogenesis. During cytokinesis, involved in cell plate
CC initiation, cell plate maturation and formation of new primary cell
CC wall. {ECO:0000269|PubMed:20870962}.
CC -!- SUBUNIT: The exocyst complex is composed of SEC3, SEC5, SEC6, SEC8,
CC SEC10, EXO70A1 and EXO84B. Interacts with EXO84B. Binds to EXO70H1 AND
CC EXO70B2 (PubMed:21199889). {ECO:0000269|PubMed:20870962,
CC ECO:0000269|PubMed:21199889}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytosol {ECO:0000269|PubMed:19895414,
CC ECO:0000269|PubMed:24307681}. Cytoplasm, cytoskeleton, phragmoplast
CC {ECO:0000269|PubMed:20870962}. Secreted, cell wall
CC {ECO:0000269|PubMed:20870962}. Secreted, extracellular exosome
CC {ECO:0000269|PubMed:24307681}. Note=Localized to globular structures in
CC the perinuclear region. During cytokinesis, localizes to the nascent
CC cell plate and later to the cell plate insertion site and along the
CC post-cytokinetic wall. Shuttles from the cytoplasm to the exocyst-
CC positive organelle (EXPO) in the presence of EXO70E2 (PubMed:24307681).
CC {ECO:0000269|PubMed:20870962, ECO:0000269|PubMed:24307681}.
CC -!- SIMILARITY: Belongs to the SEC15 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAC19268.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=CAB80728.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AF069298; AAC19268.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AL161494; CAB80728.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002687; AEE82158.1; -; Genomic_DNA.
DR EMBL; CP002687; ANM67295.1; -; Genomic_DNA.
DR PIR; T01315; T01315.
DR RefSeq; NP_001319844.1; NM_001340371.1.
DR RefSeq; NP_567229.2; NM_116468.4.
DR AlphaFoldDB; F4JHH5; -.
DR SMR; F4JHH5; -.
DR BioGRID; 13351; 5.
DR IntAct; F4JHH5; 2.
DR STRING; 3702.AT4G02350.1; -.
DR iPTMnet; F4JHH5; -.
DR PaxDb; F4JHH5; -.
DR PRIDE; F4JHH5; -.
DR ProteomicsDB; 226595; -.
DR EnsemblPlants; AT4G02350.1; AT4G02350.1; AT4G02350.
DR EnsemblPlants; AT4G02350.2; AT4G02350.2; AT4G02350.
DR GeneID; 828060; -.
DR Gramene; AT4G02350.1; AT4G02350.1; AT4G02350.
DR Gramene; AT4G02350.2; AT4G02350.2; AT4G02350.
DR KEGG; ath:AT4G02350; -.
DR Araport; AT4G02350; -.
DR TAIR; locus:2133244; AT4G02350.
DR eggNOG; KOG2176; Eukaryota.
DR HOGENOM; CLU_019671_0_0_1; -.
DR InParanoid; F4JHH5; -.
DR OMA; MERACDF; -.
DR OrthoDB; 185623at2759; -.
DR PRO; PR:F4JHH5; -.
DR Proteomes; UP000006548; Chromosome 4.
DR ExpressionAtlas; F4JHH5; baseline and differential.
DR Genevisible; F4JHH5; AT.
DR GO; GO:0005856; C:cytoskeleton; IEA:UniProtKB-KW.
DR GO; GO:0005829; C:cytosol; IDA:UniProtKB.
DR GO; GO:0000145; C:exocyst; IDA:TAIR.
DR GO; GO:0070062; C:extracellular exosome; IDA:UniProtKB.
DR GO; GO:0009524; C:phragmoplast; IEA:UniProtKB-SubCell.
DR GO; GO:0005886; C:plasma membrane; HDA:TAIR.
DR GO; GO:0009506; C:plasmodesma; HDA:TAIR.
DR GO; GO:0060321; P:acceptance of pollen; IMP:TAIR.
DR GO; GO:0006887; P:exocytosis; IBA:GO_Central.
DR GO; GO:0006893; P:Golgi to plasma membrane transport; IBA:GO_Central.
DR GO; GO:0006886; P:intracellular protein transport; IEA:InterPro.
DR GO; GO:0009846; P:pollen germination; IMP:TAIR.
DR GO; GO:0009860; P:pollen tube growth; IMP:TAIR.
DR GO; GO:0090522; P:vesicle tethering involved in exocytosis; IEA:InterPro.
DR Gene3D; 1.10.357.30; -; 1.
DR Gene3D; 1.20.58.670; -; 1.
DR InterPro; IPR007225; EXOC6/Sec15.
DR InterPro; IPR042045; EXOC6/Sec15_C_dom1.
DR InterPro; IPR042044; EXOC6PINT-1/Sec15/Tip20_C_dom2.
DR InterPro; IPR046361; Sec15_C.
DR PANTHER; PTHR12702; PTHR12702; 1.
DR Pfam; PF04091; Sec15; 1.
DR PIRSF; PIRSF025007; Sec15; 1.
PE 1: Evidence at protein level;
KW Cell wall; Cytoplasm; Cytoskeleton; Exocytosis; Reference proteome;
KW Secreted; Transport.
FT CHAIN 1..787
FT /note="Exocyst complex component SEC15B"
FT /id="PRO_0000424572"
SQ SEQUENCE 787 AA; 88371 MW; DC91B59EA3203D49 CRC64;
MQSSKGRRKV GSTTAGAGID SAEKLDELLI SSAICNGEDL GPFVRKTFGT GKPETLLHHL
KFFARSKESE IEEVCKAHYQ DFIHAVDDLK SLLSDVESLK SALSDSNSKL QSVAAPLLSS
LDSLVEAQTV SKNVDLAIGA VTHCVRVMEL VSRANQHLQS GNFYMALKCV DSIESDFMEK
TPSSTLKRML ENRIPAIRSY VERKVNKEFG DWLVEIRVVS RNLGQLAIGE ASAARQREEE
LRIKQRQAEE QSRLSLRDCV YALNEEEDDE FGSGHEGSDG GSSGGGLLGF DLTPLYRAYH
IHQTLSLGDT FKQYYYNNRD LQLTSDFQIA GFFIVEDRVL RTGGGLISKL EVETLWDTAV
TKMCAVLEDQ FSRMQTANHL LLIKDYVSLL GVSLRRYGYA VDSLLEVLSK HRDKYHELLL
SDCRKQITEA LSADKFEQML MKKEYEYSMN VLSFQLQTSE IVPAFPFIAP FSTTVPDCCR
IVRSFIEDSV SFMSHGGQLD FYDVVKKYLD RLLGEVLDEA LLKLISTSVH GVSQAMQVAA
NMAVFERACD FFFRHAAHLS GVPLRMAERG RRHFPLTKSQ NTAEDTLSGM LKKKIDGFMT
LLENVNWTSD DIPQGGNEYM NEVLIYLETL VSTAQQILPA KVLKRVLRDV LAHISEKIVG
TLCGDLVKRL SMAAIKGLDV DIQLLDSFTE NLTPLLTDKE AREMKKAFVE IRQMINLLLS
SHPENFVNPV IRERSYNALD YRKVATVSEK FRDPSDSIFG TFGTRGSRQN PKNKSLDALI
KRLKDVS
