SC5A2_RABIT
ID SC5A2_RABIT Reviewed; 672 AA.
AC P26430;
DT 01-AUG-1992, integrated into UniProtKB/Swiss-Prot.
DT 01-AUG-1992, sequence version 1.
DT 03-AUG-2022, entry version 101.
DE RecName: Full=Sodium/glucose cotransporter 2;
DE Short=Na(+)/glucose cotransporter 2;
DE AltName: Full=Low affinity sodium-glucose cotransporter;
DE AltName: Full=Solute carrier family 5 member 2;
GN Name=SLC5A2; Synonyms=SNST1;
OS Oryctolagus cuniculus (Rabbit).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Lagomorpha; Leporidae; Oryctolagus.
OX NCBI_TaxID=9986;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND TISSUE SPECIFICITY.
RC TISSUE=Kidney;
RX PubMed=1740408; DOI=10.1016/s0021-9258(19)50557-9;
RA Pajor A.M., Wright E.M.;
RT "Cloning and functional expression of a mammalian Na+/nucleoside
RT cotransporter. A member of the SGLT family.";
RL J. Biol. Chem. 267:3557-3560(1992).
CC -!- FUNCTION: Electrogenic Na(+)-coupled sugar simporter that actively
CC transports D-glucose at the plasma membrane, with a Na(+) to sugar
CC coupling ratio of 1:1. Transporter activity is driven by a
CC transmembrane Na(+) electrochemical gradient set by the Na(+)/K(+) pump
CC (By similarity). Has a primary role in D-glucose reabsorption from
CC glomerular filtrate across the brush border of the early proximal
CC tubules of the kidney (By similarity). {ECO:0000250|UniProtKB:P31639,
CC ECO:0000250|UniProtKB:Q923I7}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=D-glucose(out) + Na(+)(out) = D-glucose(in) + Na(+)(in);
CC Xref=Rhea:RHEA:70571, ChEBI:CHEBI:4167, ChEBI:CHEBI:29101;
CC Evidence={ECO:0000250|UniProtKB:P31639};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:70572;
CC Evidence={ECO:0000250|UniProtKB:P31639};
CC -!- SUBUNIT: Forms a heterodimer with PDZK1IP1; this interaction enhances
CC the transporter activity over a hundred-fold.
CC {ECO:0000250|UniProtKB:P31639}.
CC -!- SUBCELLULAR LOCATION: Apical cell membrane
CC {ECO:0000250|UniProtKB:Q923I7}; Multi-pass membrane protein
CC {ECO:0000255}.
CC -!- TISSUE SPECIFICITY: Expressed in the small intestine and kidney.
CC {ECO:0000269|PubMed:1740408}.
CC -!- SIMILARITY: Belongs to the sodium:solute symporter (SSF) (TC 2.A.21)
CC family. {ECO:0000305}.
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DR EMBL; M84020; AAA31421.1; -; mRNA.
DR PIR; A42251; A42251.
DR RefSeq; NP_001076138.1; NM_001082669.1.
DR AlphaFoldDB; P26430; -.
DR SMR; P26430; -.
DR STRING; 9986.ENSOCUP00000025524; -.
DR TCDB; 2.A.21.3.3; the solute:sodium symporter (sss) family.
DR PRIDE; P26430; -.
DR GeneID; 100009386; -.
DR KEGG; ocu:100009386; -.
DR CTD; 6524; -.
DR eggNOG; KOG2349; Eukaryota.
DR InParanoid; P26430; -.
DR OrthoDB; 243316at2759; -.
DR Proteomes; UP000001811; Unplaced.
DR GO; GO:0016324; C:apical plasma membrane; ISS:UniProtKB.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005412; F:glucose:sodium symporter activity; ISS:UniProtKB.
DR GO; GO:0035623; P:renal glucose absorption; ISS:UniProtKB.
DR Gene3D; 1.20.1730.10; -; 1.
DR InterPro; IPR038377; Na/Glc_symporter_sf.
DR InterPro; IPR001734; Na/solute_symporter.
DR InterPro; IPR018212; Na/solute_symporter_CS.
DR Pfam; PF00474; SSF; 1.
DR TIGRFAMs; TIGR00813; sss; 1.
DR PROSITE; PS00456; NA_SOLUT_SYMP_1; 1.
DR PROSITE; PS00457; NA_SOLUT_SYMP_2; 1.
DR PROSITE; PS50283; NA_SOLUT_SYMP_3; 1.
PE 2: Evidence at transcript level;
KW Cell membrane; Glycoprotein; Ion transport; Membrane; Reference proteome;
KW Sodium; Sodium transport; Symport; Transmembrane; Transmembrane helix;
KW Transport.
FT CHAIN 1..672
FT /note="Sodium/glucose cotransporter 2"
FT /id="PRO_0000105374"
FT TOPO_DOM 1..25
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 26..44
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 45..61
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 62..82
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 83..102
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 103..123
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 124..168
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 169..188
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 189..205
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 206..226
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 227..270
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 271..291
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 292..314
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 315..334
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 335..422
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 423..442
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 443..454
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 455..475
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 476..525
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 526..546
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 547..650
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 651..671
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 571..601
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CARBOHYD 250
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 672 AA; 73162 MW; E2D987B03B9C57B4 CRC64;
MEEHMEAGSR LGLGDHGALI DNPADIAVIA AYFLLVIGVG LWSMCRTNRG TVGGYFLAGR
SMVWWPVGAS LFASNIGSGH FVGLAGTGAA NGLAVAGFEW NALFVVLLLG WLFAPVYLTA
GVITMPQYLR KRFGGHRIRL YLSVLSLFLY IFTKISVDMF SGAVFIQQAL GWNIYASVIA
LLGITMVYTV TGGLAALMYT DTVQTFVIIA GAFILTGYAF HEVGGYSGLF DKYMGAMTSL
TVSEDPAVGN ISSSCYRPRP DSYHLLRDPV TGDLPWPALL LGLTIVSGWY WCSDQVIVQR
CLAGRNLTHI KAGCILCGYL KLTPMFLMVM PGMISRILYP DEVACVAPEV CKRVCGTEVG
CSNIAYPRLV VKLMPNGLRG LMLAVMLAAL MSSLASIFNS SSTLFTMDIY TLRPRAGEGE
LLLVGRLWVV FIVAVSVAWL PVVQAAQGGQ LFDYIQSVSS YLAPPVSAVF VVALFVPRVN
EKGAFWGLIG GLLMGLARLI PEFSFGTGSC VRPSACPAFL CRVHYLYFAI VLFFCSGLLI
IIVSLCTAPI PRKHLHRLVF SLRHSKEERE DLDADELEAP ASPPVQNGRP EHAVEMEEPQ
APGPGLFRQC LLWFCGMNRG RAGGPAPPTQ EEEAAAARRL EDINEDPRWS RVVNLNALLM
MAVAMFFWGF YA
