SC5A3_CANLF
ID SC5A3_CANLF Reviewed; 718 AA.
AC P31637;
DT 01-JUL-1993, integrated into UniProtKB/Swiss-Prot.
DT 01-JUL-1993, sequence version 1.
DT 03-AUG-2022, entry version 143.
DE RecName: Full=Sodium/myo-inositol cotransporter;
DE Short=Na(+)/myo-inositol cotransporter;
DE AltName: Full=Sodium/myo-inositol transporter 1;
DE Short=SMIT1;
DE AltName: Full=Solute carrier family 5 member 3;
GN Name=SLC5A3; Synonyms=SMIT;
OS Canis lupus familiaris (Dog) (Canis familiaris).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Carnivora; Caniformia; Canidae; Canis.
OX NCBI_TaxID=9615;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=Cocker spaniel; TISSUE=Kidney;
RX PubMed=1372904; DOI=10.1016/s0021-9258(18)42695-6;
RA Kwon H.M., Yamauchi A., Uchida S., Preston A.S., Garcia-Perez A.,
RA Burg M.B., Handler J.S.;
RT "Cloning of the cDNA for a Na+/myo-inositol cotransporter, a hypertonicity
RT stress protein.";
RL J. Biol. Chem. 267:6297-6301(1992).
CC -!- FUNCTION: Prevents intracellular accumulation of high concentrations of
CC myo-inositol (an osmolyte) that result in impairment of cellular
CC function.
CC -!- SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
CC -!- TISSUE SPECIFICITY: Brain and kidney.
CC -!- INDUCTION: Medium hypertonicity.
CC -!- SIMILARITY: Belongs to the sodium:solute symporter (SSF) (TC 2.A.21)
CC family. {ECO:0000305}.
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DR EMBL; M85068; -; NOT_ANNOTATED_CDS; mRNA.
DR PIR; A42163; A42163.
DR RefSeq; NP_001182069.1; NM_001195140.1.
DR RefSeq; XP_005638794.1; XM_005638737.2.
DR RefSeq; XP_005638920.1; XM_005638863.2.
DR RefSeq; XP_013965244.1; XM_014109769.1.
DR RefSeq; XP_013965245.1; XM_014109770.1.
DR AlphaFoldDB; P31637; -.
DR SMR; P31637; -.
DR STRING; 9615.ENSCAFP00000014029; -.
DR TCDB; 2.A.21.3.5; the solute:sodium symporter (sss) family.
DR PaxDb; P31637; -.
DR Ensembl; ENSCAFT00000098508; ENSCAFP00000074155; ENSCAFG00000055461.
DR Ensembl; ENSCAFT00030048209; ENSCAFP00030042180; ENSCAFG00030026072.
DR Ensembl; ENSCAFT00040035080; ENSCAFP00040030541; ENSCAFG00040018967.
DR Ensembl; ENSCAFT00845045352; ENSCAFP00845035570; ENSCAFG00845025693.
DR GeneID; 445542; -.
DR KEGG; cfa:445542; -.
DR CTD; 6526; -.
DR VEuPathDB; HostDB:ENSCAFG00845025693; -.
DR eggNOG; KOG2349; Eukaryota.
DR GeneTree; ENSGT00940000161679; -.
DR HOGENOM; CLU_018808_9_2_1; -.
DR InParanoid; P31637; -.
DR OMA; YIAQRMM; -.
DR OrthoDB; 243316at2759; -.
DR TreeFam; TF352855; -.
DR Reactome; R-CFA-429593; Inositol transporters.
DR Proteomes; UP000002254; Chromosome 31.
DR Bgee; ENSCAFG00000009546; Expressed in lymph node and 41 other tissues.
DR GO; GO:0016021; C:integral component of membrane; IBA:GO_Central.
DR GO; GO:0048471; C:perinuclear region of cytoplasm; IEA:Ensembl.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0008076; C:voltage-gated potassium channel complex; IEA:Ensembl.
DR GO; GO:0015150; F:fucose transmembrane transporter activity; IDA:ARUK-UCL.
DR GO; GO:0005412; F:glucose:sodium symporter activity; IDA:ARUK-UCL.
DR GO; GO:0005367; F:myo-inositol:sodium symporter activity; IDA:ARUK-UCL.
DR GO; GO:0015146; F:pentose transmembrane transporter activity; IDA:ARUK-UCL.
DR GO; GO:0015166; F:polyol transmembrane transporter activity; IDA:ARUK-UCL.
DR GO; GO:0015459; F:potassium channel regulator activity; IEA:Ensembl.
DR GO; GO:0044325; F:transmembrane transporter binding; IEA:Ensembl.
DR GO; GO:0015756; P:fucose transmembrane transport; IDA:ARUK-UCL.
DR GO; GO:1904659; P:glucose transmembrane transport; IDA:ARUK-UCL.
DR GO; GO:0006020; P:inositol metabolic process; IBA:GO_Central.
DR GO; GO:0015798; P:myo-inositol transport; IDA:ARUK-UCL.
DR GO; GO:0015750; P:pentose transmembrane transport; IDA:ARUK-UCL.
DR GO; GO:0007422; P:peripheral nervous system development; IEA:Ensembl.
DR GO; GO:0015791; P:polyol transmembrane transport; IDA:ARUK-UCL.
DR GO; GO:0043085; P:positive regulation of catalytic activity; IEA:Ensembl.
DR GO; GO:1905477; P:positive regulation of protein localization to membrane; IEA:Ensembl.
DR GO; GO:1903428; P:positive regulation of reactive oxygen species biosynthetic process; IEA:Ensembl.
DR GO; GO:0043576; P:regulation of respiratory gaseous exchange; IEA:Ensembl.
DR GO; GO:0032409; P:regulation of transporter activity; IEA:Ensembl.
DR CDD; cd11491; SLC5sbd_SMIT; 1.
DR Gene3D; 1.20.1730.10; -; 1.
DR InterPro; IPR038377; Na/Glc_symporter_sf.
DR InterPro; IPR001734; Na/solute_symporter.
DR InterPro; IPR018212; Na/solute_symporter_CS.
DR InterPro; IPR042731; SMIT.
DR Pfam; PF00474; SSF; 1.
DR TIGRFAMs; TIGR00813; sss; 1.
DR PROSITE; PS00456; NA_SOLUT_SYMP_1; 1.
DR PROSITE; PS00457; NA_SOLUT_SYMP_2; 1.
DR PROSITE; PS50283; NA_SOLUT_SYMP_3; 1.
PE 2: Evidence at transcript level;
KW Glycoprotein; Ion transport; Membrane; Phosphoprotein; Reference proteome;
KW Sodium; Sodium transport; Symport; Transmembrane; Transmembrane helix;
KW Transport.
FT CHAIN 1..718
FT /note="Sodium/myo-inositol cotransporter"
FT /id="PRO_0000105380"
FT TOPO_DOM 1..9
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 10..29
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 30..38
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 39..57
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 58..86
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 87..110
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 111..123
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 124..144
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 145..157
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 158..183
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 184..186
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 187..205
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 206..303
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 304..324
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 325..353
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 354..376
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 377..406
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 407..430
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 431..443
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 444..462
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 463..510
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 511..532
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 533..695
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 696..716
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 717..718
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT SITE 24
FT /note="Implicated in sodium coupling"
FT /evidence="ECO:0000250"
FT SITE 285
FT /note="Implicated in sodium coupling"
FT /evidence="ECO:0000250"
FT MOD_RES 594
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:P53794"
FT MOD_RES 632
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:P53794"
FT CARBOHYD 232
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 718 AA; 79546 MW; 4C1B5CC4485CD268 CRC64;
MRAVLETADI AIVALYFILV MCIGFFAMWK SNRSTVSGYF LAGRSMTWVA IGASLFVSNI
GSEHFIGLAG SGAASGFAVG AWEFNALLLL QLLGWVFIPI YIRSGVYTMP EYLSKRFGGH
RIQVYFAALS LILYIFTKLS VDLYSGALFI QESLGWNLYV SVILLIGMTA LLTVTGGLVA
VIYTDTLQAL LMIVGALTLM IISMMEIGGF EEVKRRYMLA SPNVTSILLT YNLSNTNSCN
VHPKKDALKM LRNPTDEDVP WPGFVLGQTP ASVWYWCADQ VIVQRVLAAK NIAHAKGSTL
MAGFLKLLPM FIIVVPGMIS RILFADDIAC INPEHCMQVC GSRAGCSNIA YPRLVMKLVP
VGLRGLMMAV MIAALMSDLD SIFNSASTIF TLDVYKLIRR SASSRELMIV GRIFVAFMVV
ISIAWVPIIV EMQGGQMYLY IQEVADYLTP PVAALFLLAI FWKRCNEQGA FYGGMAGFVL
GAVRLTLAFA YRAPECDQPD NRPGFIKDIH YMYVATALFW VTGLITVIVS LLTPPPTKEQ
IRTTTFWSKK SLVVKESCSP KDEPYKMQEK SILRCSENSE ATNHIIPNGK SEDSIKGLQP
EDVNLLVTCR EEGNPVASLG HSEAETPVDA YSNGQAALMG EKERKKETED GGRYWKFIDW
FCGFKSKSLS KRSLRDLMEE EAVCLQMLEE PPQVKLILNI GLFAVCSLGI FMFVYFSL
