SC5A3_MOUSE
ID SC5A3_MOUSE Reviewed; 718 AA.
AC Q9JKZ2; B2RU60;
DT 01-JUN-2001, integrated into UniProtKB/Swiss-Prot.
DT 27-JUL-2011, sequence version 2.
DT 03-AUG-2022, entry version 144.
DE RecName: Full=Sodium/myo-inositol cotransporter;
DE Short=Na(+)/myo-inositol cotransporter;
DE AltName: Full=Sodium/myo-inositol transporter 1;
DE Short=SMIT1;
DE AltName: Full=Solute carrier family 5 member 3;
GN Name=Slc5a3;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=10773690; DOI=10.1159/000015509;
RA McVeigh K.E., Mallee J.J., Lucente A., Barnoski B.L., Wu S., Berry G.T.;
RT "Murine chromosome 16 telomeric region, homologous with human chromosome
RT 21q22, contains the osmoregulatory Na(+)/myo-inositol cotransporter
RT (SLC5A3) gene.";
RL Cytogenet. Cell Genet. 88:153-158(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.;
RL Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP FUNCTION AS M813 MURINE LEUKEMIA VIRUS RECEPTOR, AND TOPOLOGY.
RX PubMed=12719585; DOI=10.1128/jvi.77.10.5926-5932.2003;
RA Hein S., Prassolov V., Zhang Y., Ivanov D., Lohler J., Ross S.R.,
RA Stocking C.;
RT "Sodium-dependent myo-inositol transporter 1 is a cellular receptor for Mus
RT cervicolor M813 murine leukemia virus.";
RL J. Virol. 77:5926-5932(2003).
RN [5]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19144319; DOI=10.1016/j.immuni.2008.11.006;
RA Trost M., English L., Lemieux S., Courcelles M., Desjardins M.,
RA Thibault P.;
RT "The phagosomal proteome in interferon-gamma-activated macrophages.";
RL Immunity 30:143-154(2009).
RN [6]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Kidney;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Prevents intracellular accumulation of high concentrations of
CC myo-inositol (an osmolyte) that result in impairment of cellular
CC function. {ECO:0000269|PubMed:12719585}.
CC -!- FUNCTION: Functions as a retroviral receptor for M813 murine leukemia
CC virus (MuLV) entry. {ECO:0000269|PubMed:12719585}.
CC -!- SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
CC -!- SIMILARITY: Belongs to the sodium:solute symporter (SSF) (TC 2.A.21)
CC family. {ECO:0000305}.
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DR EMBL; AF220915; AAF43668.1; -; Genomic_DNA.
DR EMBL; CH466602; EDL03793.1; -; Genomic_DNA.
DR EMBL; BC140982; AAI40983.1; -; mRNA.
DR EMBL; BC140983; AAI40984.1; -; mRNA.
DR CCDS; CCDS37403.1; -.
DR RefSeq; NP_059087.2; NM_017391.3.
DR AlphaFoldDB; Q9JKZ2; -.
DR SMR; Q9JKZ2; -.
DR STRING; 10090.ENSMUSP00000109608; -.
DR GlyGen; Q9JKZ2; 1 site.
DR iPTMnet; Q9JKZ2; -.
DR PhosphoSitePlus; Q9JKZ2; -.
DR SwissPalm; Q9JKZ2; -.
DR EPD; Q9JKZ2; -.
DR MaxQB; Q9JKZ2; -.
DR PaxDb; Q9JKZ2; -.
DR PeptideAtlas; Q9JKZ2; -.
DR PRIDE; Q9JKZ2; -.
DR ProteomicsDB; 256604; -.
DR Antibodypedia; 8029; 209 antibodies from 28 providers.
DR DNASU; 53881; -.
DR Ensembl; ENSMUST00000113975; ENSMUSP00000109608; ENSMUSG00000089774.
DR GeneID; 53881; -.
DR KEGG; mmu:53881; -.
DR UCSC; uc007zyt.2; mouse.
DR CTD; 6526; -.
DR MGI; MGI:1858226; Slc5a3.
DR VEuPathDB; HostDB:ENSMUSG00000089774; -.
DR eggNOG; KOG2349; Eukaryota.
DR GeneTree; ENSGT00940000161679; -.
DR HOGENOM; CLU_018808_9_2_1; -.
DR InParanoid; Q9JKZ2; -.
DR OMA; IHFMYVA; -.
DR OrthoDB; 243316at2759; -.
DR PhylomeDB; Q9JKZ2; -.
DR TreeFam; TF352855; -.
DR Reactome; R-MMU-429593; Inositol transporters.
DR BioGRID-ORCS; 53881; 2 hits in 70 CRISPR screens.
DR PRO; PR:Q9JKZ2; -.
DR Proteomes; UP000000589; Chromosome 16.
DR RNAct; Q9JKZ2; protein.
DR Bgee; ENSMUSG00000089774; Expressed in vestibular membrane of cochlear duct and 246 other tissues.
DR Genevisible; Q9JKZ2; MM.
DR GO; GO:0016021; C:integral component of membrane; IBA:GO_Central.
DR GO; GO:0048471; C:perinuclear region of cytoplasm; ISO:MGI.
DR GO; GO:0005886; C:plasma membrane; ISO:MGI.
DR GO; GO:0008076; C:voltage-gated potassium channel complex; IDA:MGI.
DR GO; GO:0005412; F:glucose:sodium symporter activity; IBA:GO_Central.
DR GO; GO:0015459; F:potassium channel regulator activity; ISO:MGI.
DR GO; GO:0044325; F:transmembrane transporter binding; ISO:MGI.
DR GO; GO:0006020; P:inositol metabolic process; IMP:MGI.
DR GO; GO:0015798; P:myo-inositol transport; IMP:MGI.
DR GO; GO:0007422; P:peripheral nervous system development; IMP:MGI.
DR GO; GO:0043085; P:positive regulation of catalytic activity; IMP:ARUK-UCL.
DR GO; GO:1905477; P:positive regulation of protein localization to membrane; IMP:ARUK-UCL.
DR GO; GO:1903428; P:positive regulation of reactive oxygen species biosynthetic process; IMP:ARUK-UCL.
DR GO; GO:0043576; P:regulation of respiratory gaseous exchange; IMP:MGI.
DR GO; GO:0032409; P:regulation of transporter activity; IDA:MGI.
DR CDD; cd11491; SLC5sbd_SMIT; 1.
DR Gene3D; 1.20.1730.10; -; 1.
DR InterPro; IPR038377; Na/Glc_symporter_sf.
DR InterPro; IPR001734; Na/solute_symporter.
DR InterPro; IPR018212; Na/solute_symporter_CS.
DR InterPro; IPR042731; SMIT.
DR Pfam; PF00474; SSF; 1.
DR TIGRFAMs; TIGR00813; sss; 1.
DR PROSITE; PS00456; NA_SOLUT_SYMP_1; 1.
DR PROSITE; PS00457; NA_SOLUT_SYMP_2; 1.
DR PROSITE; PS50283; NA_SOLUT_SYMP_3; 1.
PE 1: Evidence at protein level;
KW Glycoprotein; Ion transport; Membrane; Phosphoprotein; Reference proteome;
KW Sodium; Sodium transport; Symport; Transmembrane; Transmembrane helix;
KW Transport.
FT CHAIN 1..718
FT /note="Sodium/myo-inositol cotransporter"
FT /id="PRO_0000105382"
FT TOPO_DOM 1..9
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 10..29
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 30..38
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 39..57
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 58..86
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 87..110
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 111..123
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 124..144
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 145..157
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 158..183
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 184..186
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 187..205
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 206..303
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 304..324
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 325..353
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 354..376
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 377..406
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 407..430
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 431..443
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 444..462
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 463..510
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 511..532
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 533..695
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 696..716
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 717..718
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT SITE 24
FT /note="Implicated in sodium coupling"
FT /evidence="ECO:0000250"
FT SITE 285
FT /note="Implicated in sodium coupling"
FT /evidence="ECO:0000250"
FT MOD_RES 594
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:P53794"
FT MOD_RES 632
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:P53794"
FT CARBOHYD 232
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CONFLICT 653
FT /note="R -> Q (in Ref. 1; AAF43668)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 718 AA; 79583 MW; D0371D6ECF08503B CRC64;
MRAVLEAADI AVVALYFILV MCIGFFAMWK SNRSTVSGYF LAGRSMTWVA IGASLFVSNI
GSEHFIGLAG SGAASGFAVG AWEFNALLLL QLLGWVFIPI YIRSGVYTMP EYLSKRFGGH
RIQVYFAALS LLLYIFTKLS VDLYSGALFI QESLGWNLYV SVILLIGMTA LLTVTGGLVA
VIYTDTLQAL LMIIGALTLM VISMVKIGGF EEVKRRYMLA SPDVASILLK YNLSNTNACM
VHPKANALKM LRDPTDEDVP WPGFILGQTP ASVWYWCADQ VIVQRVLAAK NIAHAKGSTL
MAGFLKLLPM FIIVVPGMIS RIVFADEIAC INPEHCMQVC GSRAGCSNIA YPRLVMTLVP
VGLRGLMMAV MIAALMSDLD SIFNSASTIF TLDVYKLIRK SASSRELMIV GRIFVAFMVV
ISIAWVPIIV EMQGGQMYLY IQEVADYLTP PVAALFLLAI FWKRCNEQGA FYGGMAGFVL
GAVRLILAFT YRAPECDQPD NRPGFIKDIH YMYVATALFW ITGLITVIVS LLTPPPTKDQ
IRTTTFWSKK TLVTKESCSQ KDEPYKMQEK SILQCSENSE VISHTIPNGK SEDSIKGLQP
EDVNLLVTCR EEGNPVASMG HSEAETPVDA YSNGQAALMG EREREKETEN RSRYWKFIDW
FCGFKSKSLS KRSLRDLMDE EAVCLQMLEE TPQVKVILNI GLFAVCSLGI FMFVYFSL
