SC5A4_PIG
ID SC5A4_PIG Reviewed; 660 AA.
AC P31636;
DT 01-JUL-1993, integrated into UniProtKB/Swiss-Prot.
DT 01-JUL-1993, sequence version 1.
DT 25-MAY-2022, entry version 105.
DE RecName: Full=Solute carrier family 5 member 4 {ECO:0000305};
DE AltName: Full=Low affinity sodium-glucose cotransporter {ECO:0000303|PubMed:8077195};
GN Name=SLC5A4; Synonyms=SAAT1, SGLT3;
OS Sus scrofa (Pig).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Artiodactyla; Suina; Suidae; Sus.
OX NCBI_TaxID=9823;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND TISSUE SPECIFICITY.
RC TISSUE=Kidney;
RX PubMed=8420925; DOI=10.1016/s0021-9258(18)53880-1;
RA Kong C.-T., Yet S.-F., Lever J.E.;
RT "Cloning and expression of a mammalian Na+/amino acid cotransporter with
RT sequence similarity to Na+/glucose cotransporters.";
RL J. Biol. Chem. 268:1509-1512(1993).
RN [2]
RP FUNCTION, AND ACTIVITY REGULATION.
RX PubMed=8077195; DOI=10.1016/s0021-9258(17)31672-1;
RA McKenzie B., Panayotova-Heiermann M., Loo D.D.F., Lever J.E., Wright E.M.;
RT "SAAT1 is a low affinity Na+/glucose cotransporter and not an amino acid
RT transporter. A reinterpretation.";
RL J. Biol. Chem. 269:22488-22491(1994).
RN [3]
RP FUNCTION.
RC TISSUE=Small intestine;
RX PubMed=13130073; DOI=10.1073/pnas.1733027100;
RA Diez-Sampedro A., Hirayama B.A., Osswald C., Gorboulev V., Baumgarten K.,
RA Volk C., Wright E.M., Koepsell H.;
RT "A glucose sensor hiding in a family of transporters.";
RL Proc. Natl. Acad. Sci. U.S.A. 100:11753-11758(2003).
CC -!- FUNCTION: Has electrogenic activity in response to glucose, and may
CC function as a glucose sensor (PubMed:8077195). Also has low-affinity
CC sodium/glucose cotransporter activity; sugar transport activity is
CC tightly coupled to ion transport at neutral pH but is reduced under
CC more acidic conditions (PubMed:8077195, PubMed:13130073).
CC {ECO:0000269|PubMed:13130073, ECO:0000269|PubMed:8077195}.
CC -!- ACTIVITY REGULATION: Inhibited by phlorizin.
CC {ECO:0000269|PubMed:8077195}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305|PubMed:8077195,
CC ECO:0000305|PubMed:8420925}; Multi-pass membrane protein {ECO:0000255}.
CC -!- TISSUE SPECIFICITY: Kidney, intestine, liver, skeletal muscle and
CC spleen. {ECO:0000269|PubMed:8420925}.
CC -!- SIMILARITY: Belongs to the sodium:solute symporter (SSF) (TC 2.A.21)
CC family. {ECO:0000305}.
CC -!- CAUTION: Was originally thought to be a sodium/neutral amino acid
CC cotransporter (system a neutral amino acid transporter) responsible for
CC the sodium-dependent intake of neutral amino acids such as alanine,
CC glycine, serine, cysteine, and proline. {ECO:0000305|PubMed:8420925}.
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DR EMBL; L02900; AAC37325.1; -; mRNA.
DR PIR; A44432; A44432.
DR RefSeq; NP_999347.1; NM_214182.1.
DR AlphaFoldDB; P31636; -.
DR SMR; P31636; -.
DR STRING; 9823.ENSSSCP00000010710; -.
DR TCDB; 2.A.21.3.4; the solute:sodium symporter (sss) family.
DR PaxDb; P31636; -.
DR PeptideAtlas; P31636; -.
DR PRIDE; P31636; -.
DR Ensembl; ENSSSCT00005065693; ENSSSCP00005040644; ENSSSCG00005040678.
DR Ensembl; ENSSSCT00055022062; ENSSSCP00055017465; ENSSSCG00055011006.
DR GeneID; 397376; -.
DR KEGG; ssc:397376; -.
DR CTD; 6527; -.
DR eggNOG; KOG2349; Eukaryota.
DR InParanoid; P31636; -.
DR OrthoDB; 243316at2759; -.
DR PRO; PR:P31636; -.
DR Proteomes; UP000008227; Unplaced.
DR Proteomes; UP000314985; Unplaced.
DR GO; GO:0016021; C:integral component of membrane; IBA:GO_Central.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0005412; F:glucose:sodium symporter activity; IBA:GO_Central.
DR GO; GO:0006814; P:sodium ion transport; IBA:GO_Central.
DR Gene3D; 1.20.1730.10; -; 1.
DR InterPro; IPR038377; Na/Glc_symporter_sf.
DR InterPro; IPR001734; Na/solute_symporter.
DR InterPro; IPR018212; Na/solute_symporter_CS.
DR Pfam; PF00474; SSF; 1.
DR TIGRFAMs; TIGR00813; sss; 1.
DR PROSITE; PS00456; NA_SOLUT_SYMP_1; 1.
DR PROSITE; PS00457; NA_SOLUT_SYMP_2; 1.
DR PROSITE; PS50283; NA_SOLUT_SYMP_3; 1.
PE 2: Evidence at transcript level;
KW Cell membrane; Glycoprotein; Ion transport; Membrane; Reference proteome;
KW Sodium; Sodium transport; Sugar transport; Symport; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..660
FT /note="Solute carrier family 5 member 4"
FT /id="PRO_0000105378"
FT TOPO_DOM 1..28
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 29..47
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 48..64
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 65..85
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 86..105
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 106..126
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 127..171
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 172..191
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 192..208
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 209..229
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 230..270
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 271..291
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 292..314
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 315..334
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 335..423
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 424..443
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 444..455
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 456..476
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 477..526
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 527..547
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 548..638
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 639..659
FT /note="Helical"
FT /evidence="ECO:0000255"
FT CARBOHYD 248
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 660 AA; 72745 MW; 38616367F8F18F1A CRC64;
MASTLSPSTV TKTPGPPEIS ERIQNAADIS VIVIYFVVVM AVGLWAMLRT NRGTVGGFFL
AGRDVTWWPM GASLFASNIG SGHFVGLAGT GAASGIAIAA FEWNALLLLL VLGWFFVPIY
IKAGVMTMPE YLRKRFGGKR LQIYLSILSL FICVALRISS DIFSGAIFIK LALGLDLYLA
IFSLLAITAI YTITGGLASV IYTDTLQTII MLIGSFILMG FAFVEVGGYE SFTEKYMNAI
PTIVEGDNLT ISPKCYTPQG DSFHIFRDAV TGDIPWPGMI FGMTVVAAWY WCTDQVIVQR
CLSGKDMSHV KAACIMCGYL KLLPMFLMVM PGMISRILYT EKVACVVPSE CVKHCGTEVG
CSNYAYPLLV MELMPSGLRG LMLSVMLASL MSSLTSIFNS ASTLFTMDLY TKIRKQASEK
ELLIAGRLFI ILLIVISIVW VPLVQVAQNG QLFHYIESIS SYLGPPIAAV FLLAIFCKRV
NEQGAFWGLI IGFVMGLIRM IAEFVYGTGS CLAASNCPQI ICGVHYLYFA LILFFVSILV
VLAISLLTKP IPDVHLYRLC WALRNSTEER IDLDAEEKRH EEAHDGVDED NPEETRGCLR
KAYDLFCGLQ RKGPKLSKEE EEAQKRKLTD TSEKPLWKTI VNINAILLLA VAVFVHGYFA
