SC5A5_MOUSE
ID SC5A5_MOUSE Reviewed; 618 AA.
AC Q99PN0; B2RPX6;
DT 15-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 25-MAY-2022, entry version 115.
DE RecName: Full=Sodium/iodide cotransporter;
DE Short=Na(+)/I(-) cotransporter;
DE AltName: Full=Sodium-iodide symporter;
DE Short=Na(+)/I(-) symporter;
DE AltName: Full=Solute carrier family 5 member 5;
GN Name=Slc5a5; Synonyms=Nis;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=C57BL/6 X DBA/2;
RA Perron B., Rodriguez A.-M., Leblanc G., Pourcher T.;
RT "Cloning of the mouse sodium iodide symporter (mNIS) and its expression in
RT the mammary gland.";
RL Submitted (FEB-2000) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=BALB/cJ;
RX PubMed=11716040; DOI=10.1089/105072501753210993;
RA Pinke L.A., Dean D.S., Bergert E.R., Spitzweg C., Dutton C.M., Morris J.C.;
RT "Cloning of the mouse sodium iodide symporter.";
RL Thyroid 11:935-939(2001).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: Mediates iodide uptake in the thyroid gland.
CC -!- SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
CC -!- SIMILARITY: Belongs to the sodium:solute symporter (SSF) (TC 2.A.21)
CC family. {ECO:0000305}.
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DR EMBL; AF235001; AAK00232.1; -; mRNA.
DR EMBL; AF380353; AAK59274.1; -; mRNA.
DR EMBL; BC137650; AAI37651.1; -; mRNA.
DR EMBL; BC137651; AAI37652.1; -; mRNA.
DR CCDS; CCDS22385.1; -.
DR AlphaFoldDB; Q99PN0; -.
DR SMR; Q99PN0; -.
DR STRING; 10090.ENSMUSP00000000809; -.
DR GlyGen; Q99PN0; 2 sites.
DR PhosphoSitePlus; Q99PN0; -.
DR PaxDb; Q99PN0; -.
DR PRIDE; Q99PN0; -.
DR ProteomicsDB; 256605; -.
DR MGI; MGI:2149330; Slc5a5.
DR eggNOG; KOG2349; Eukaryota.
DR InParanoid; Q99PN0; -.
DR PhylomeDB; Q99PN0; -.
DR Reactome; R-MMU-209968; Thyroxine biosynthesis.
DR Reactome; R-MMU-428643; Organic anion transporters.
DR PRO; PR:Q99PN0; -.
DR Proteomes; UP000000589; Unplaced.
DR RNAct; Q99PN0; protein.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005634; C:nucleus; ISO:MGI.
DR GO; GO:0005886; C:plasma membrane; ISO:MGI.
DR GO; GO:0015111; F:iodide transmembrane transporter activity; ISO:MGI.
DR GO; GO:0008507; F:sodium:iodide symporter activity; IDA:MGI.
DR GO; GO:0015293; F:symporter activity; IDA:MGI.
DR GO; GO:1904200; P:iodide transmembrane transport; ISO:MGI.
DR GO; GO:0015705; P:iodide transport; ISO:MGI.
DR GO; GO:0006814; P:sodium ion transport; ISO:MGI.
DR GO; GO:0006590; P:thyroid hormone generation; IEA:InterPro.
DR CDD; cd11503; SLC5sbd_NIS; 1.
DR Gene3D; 1.20.1730.10; -; 1.
DR InterPro; IPR038377; Na/Glc_symporter_sf.
DR InterPro; IPR001734; Na/solute_symporter.
DR InterPro; IPR018212; Na/solute_symporter_CS.
DR InterPro; IPR035689; SLC5A5.
DR Pfam; PF00474; SSF; 1.
DR TIGRFAMs; TIGR00813; sss; 1.
DR PROSITE; PS00456; NA_SOLUT_SYMP_1; 1.
DR PROSITE; PS50283; NA_SOLUT_SYMP_3; 1.
PE 2: Evidence at transcript level;
KW Glycoprotein; Ion transport; Membrane; Phosphoprotein; Reference proteome;
KW Sodium; Sodium transport; Symport; Transmembrane; Transmembrane helix;
KW Transport.
FT CHAIN 1..618
FT /note="Sodium/iodide cotransporter"
FT /id="PRO_0000105384"
FT TOPO_DOM 1..16
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 17..37
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 38..53
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 54..74
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 75..88
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 89..109
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 110..136
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 137..157
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 158..163
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 164..184
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 185..186
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 187..207
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 208..241
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 242..262
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 263..286
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 287..307
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 308..326
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 327..347
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 348..391
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 392..412
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 413..416
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 417..437
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 438..444
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 445..465
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 466..520
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 521..541
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 542..618
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT REGION 571..618
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 551
FT /note="Phosphoserine; by PKA"
FT /evidence="ECO:0000255"
FT CARBOHYD 485
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 497
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 618 AA; 65568 MW; 448AC5FE9AB5118E CRC64;
MEGAEAGARA TFGPWDYGVF ATMLLVSTGI GLWVGLARGG QRSADDFFTG GRQLAAVPVG
LSLAASFMSA VQVLGVPAEA ARYGLKFLWM CVGQLLNSLL TALLFLPIFY RLGLTSTYQY
LELRFSRAVR LCGTLQYLVA TMLYTGIVIY APALILNQVT GLDIWASLLS TGIICTLYTT
VGGMKAVVWT DVFQVVVMLV GFWVILARGV MLMGGPWNVL SLAQNHSRIN LMDFDPDPRS
RYTFWTFVVG GSLVWLSMYG VNQAQVQRYV ACHTERKAKL ALLVNQLGLF LIVASAACCG
IVMFVYYKDC DPLLTGRIAA PDQYMPLLVL DIFEDLPGVP GLFLACAYSG TLSTASTSIN
AMAAVTVEDL IKPRMPSLAP RKLVFISKGL SFIYGSTCLT VAALSSLLGG GVLQGSFTVM
GVISGPLLGA FTLGMLLPAC NTPGVLSGLT AGLAVSLWVA VGATLYPPGE QTMGVLPTSA
AGCTNASVLP SPPGAANTSR GIPSSGMDSG RPAFADTFYA VSYLYYGALG TLTTMLCGAL
ISYLTGPTKR SSLGPGLLWW DLARQTASVA PKEDTTTLED SLVKGPEDIP AATKKPPGFR
PEAETHPLYL GHDVETNL
