SC5A7_TORMA
ID SC5A7_TORMA Reviewed; 584 AA.
AC Q8UWF0;
DT 07-DEC-2004, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2002, sequence version 1.
DT 25-MAY-2022, entry version 54.
DE RecName: Full=High-affinity choline transporter 1;
GN Name=CHT1;
OS Torpedo marmorata (Marbled electric ray).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Chondrichthyes;
OC Elasmobranchii; Batoidea; Torpediniformes; Torpedinidae; Torpedo.
OX NCBI_TaxID=7788;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, AND TISSUE SPECIFICITY.
RC TISSUE=Electric lobe;
RX PubMed=12358793; DOI=10.1046/j.1471-4159.2002.01044.x;
RA Lane-Guermonprez L., O'Regan S., Meunier F.-M., Morot-Gaudry-Talarmain Y.;
RT "The neuronal choline transporter CHT1 is regulated by immunosuppressor-
RT sensitive pathways.";
RL J. Neurochem. 82:874-884(2002).
CC -!- FUNCTION: Imports choline from the extracellular space to the neuron
CC with high affinity. Rate-limiting step in acetylcholine synthesis.
CC Sodium ion and chloride ion dependent. {ECO:0000269|PubMed:12358793}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000250}; Multi-pass membrane
CC protein {ECO:0000250}.
CC -!- TISSUE SPECIFICITY: Specific for cholinergic neurons.
CC {ECO:0000269|PubMed:12358793}.
CC -!- PTM: Phosphorylated. {ECO:0000250}.
CC -!- MISCELLANEOUS: Specifically inhibited by nanomolar concentrations of
CC hemicholinium 3.
CC -!- SIMILARITY: Belongs to the sodium:solute symporter (SSF) (TC 2.A.21)
CC family. {ECO:0000305}.
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DR EMBL; AJ420808; CAD12727.1; -; mRNA.
DR AlphaFoldDB; Q8UWF0; -.
DR SMR; Q8UWF0; -.
DR GO; GO:0016021; C:integral component of membrane; NAS:UniProtKB.
DR GO; GO:0015220; F:choline transmembrane transporter activity; IMP:UniProtKB.
DR GO; GO:0015293; F:symporter activity; IEA:UniProtKB-KW.
DR GO; GO:0008292; P:acetylcholine biosynthetic process; IMP:UniProtKB.
DR GO; GO:0006814; P:sodium ion transport; IEA:UniProtKB-KW.
DR Gene3D; 1.20.1730.10; -; 1.
DR InterPro; IPR038377; Na/Glc_symporter_sf.
DR InterPro; IPR001734; Na/solute_symporter.
DR Pfam; PF00474; SSF; 1.
DR PROSITE; PS50283; NA_SOLUT_SYMP_3; 1.
PE 2: Evidence at transcript level;
KW Glycoprotein; Ion transport; Membrane; Neurotransmitter biosynthesis;
KW Phosphoprotein; Sodium; Sodium transport; Symport; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..584
FT /note="High-affinity choline transporter 1"
FT /id="PRO_0000105394"
FT TOPO_DOM 1..6
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 7..27
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 28..50
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 51..71
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 72..83
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 84..104
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 105..127
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 128..148
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 149..166
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 167..187
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 188..193
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 194..214
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 215..239
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 240..260
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 261..276
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 277..297
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 298..319
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 320..340
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 341..378
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 379..399
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 400..408
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 409..429
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 430..437
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 438..458
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 459..487
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 488..508
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 509..584
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT CARBOHYD 303
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 584 AA; 63660 MW; 995F937B01195A3D CRC64;
MTVHIDGIVA IVLFYLLILF VGLWAAWKSK NTSMEGAMDR SEAIMIGGRD IGLLVGGFTM
TATWVGGGYI NGTAEAVYVP GYGLAWAQAP FGYALSLVIG GLFFAKPMRS RGYVTMLDPF
QQMYGKRMGG LLFIPALLGE IFWSAAILSA LGATLSVIVD ININVSVVVS AVIAVLYTLV
GGLYSVAYTD VVQLFCIFLG LWISIPFALL NPAVTDIIVT ANQEVYQEPW VGNIQSKDSL
IWIDNFLLLM LGGIPWQVYF QRVLSASSAT YAQVLSFLAA FGCVLMAIPS VLIGAIGTST
DWNQTSYGLP GPIGKNETDM ILPIVLQHLC PPYISFFGLG AVSAAVMSSA DSSILSASSM
FARNIYHLAF RQEASDKEIV WVMRITIFLF GGAATSMALL AQSIYGLWYL SSDLVYVIIF
PQLISVLFVK GTNTYGSIAG YIIGFLLRIS GGEPYLHMQP FIYYPGCYLD HSFGDDPVYV
QRFPFKTMAM LFSFLGNTGV SYLVKYLFVS GILPPKLDFL DSVVSKHSKE IMDKTFLMNQ
DNITLSELVH VNPIHSASVS AALTNKEAFE DIEPNPELSK SGND
